Identification of quantitative trait loci for body temperature, body weight, breast yield, and digestibility in an advanced intercross line of chickens under heat stress

Goor, Angelica Van; Bolek, Kevin J.; Ashwell, C. M.; Persia, Michael E.; Rothschild, Max F.; Schmidt, Carl J.; Lamont, Susan J.

doi:10.1186/s12711-015-0176-7

Cited by 71 publications

(62 citation statements)

References 64 publications

(71 reference statements)

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“…The 3 distinct genetic lines maintained at Iowa State University that were used in this study were a heat-susceptible broiler line, the heat-resistant Fayoumi, and the F 19 generation of a highly advanced intercross line (AIL) between the broiler and Fayoumi [8, 18]. The Fayoumi line originated from a hot climate (desert region of Egypt) and has not undergone selection for production traits.…”

Section: Methodsmentioning

confidence: 99%

“…The Fayoumi line originated from a hot climate (desert region of Egypt) and has not undergone selection for production traits. This line provides a unique genetic resource for exploring the genes related to heat stress resistance [8]. The AIL of 19 generations of intercrossing between two highly divergent chicken lines (broiler x Fayoumi) were created to help identify and map the genetic loci associated with various traits, including heat tolerance [8].…”

Section: Methodsmentioning

confidence: 99%

“…This line provides a unique genetic resource for exploring the genes related to heat stress resistance [8]. The AIL of 19 generations of intercrossing between two highly divergent chicken lines (broiler x Fayoumi) were created to help identify and map the genetic loci associated with various traits, including heat tolerance [8]. At 17 days of age, male birds were transferred to temperature-controlled chambers and acclimated to the new environment for five days.…”

Section: Methodsmentioning

confidence: 99%

“…At 17 days of age, male birds were transferred to temperature-controlled chambers and acclimated to the new environment for five days. Experimental design details were previously published in a report of a study [8] that characterized immune tissue samples collected from this experiment. From 22 to 28 days of age, the heat stress group ( n = 24) were exposed to high ambient temperature (35 °C) for 7 h per day and remained at 25 °C at all other times.…”

Section: Methodsmentioning

confidence: 99%

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Liver transcriptome response to hyperthermic stress in three distinct chicken lines

et al. 2016

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BackgroundHigh ambient temperatures cause stress in poultry, especially for broiler lines, which are genetically selected for rapid muscle growth. RNA-seq technology provides powerful insights into environmental response from a highly metabolic tissue, the liver. We investigated the effects of acute (3 h, 35 °C) and chronic (7d of 35 °C for 7 h/d) heat stress on the liver transcriptome of 3-week-old chicks of a heat-susceptible broiler line, a heat-resistant Fayoumi line, and their advanced intercross line (AIL).ResultsTranscriptome sequencing of 48 male chickens using Illumina HiSeq 2500 technology yielded an average of 33.9 million, 100 base-pair, single-end reads per sample. There were 8 times more differentially expressed genes (DEGs) (FDR < 0.05) in broilers (n = 627) than Fayoumis (n = 78) when comparing the acute-heat samples to the control (25 °C) samples. Contrasting genetic lines under similar heat treatments, the highest number of DEGs appeared between Fayoumi and broiler lines. Principal component analysis of gene expression and analysis of the number of DEGs suggested that the AIL had a transcriptomic response more similar to the Fayoumi than the broiler line during acute heat stress. The number of DEGs also suggested that acute heat stress had greater impact on the broiler liver transcriptome than chronic heat stress. The angiopoietin-like 4 (ANGPTL4) gene was identified as differentially expressed among all 6 contrasts. Ingenuity Pathway Analysis (IPA) created a novel network that combines the heat shock protein family with immune response genes.ConclusionsThis study extends our understanding of the liver transcriptome response to different heat exposure treatments in distinct genetic chicken lines and provides information necessary for breeding birds to be more resilient to the negative impacts of heat. The data strongly suggest ANGPTL4 as a candidate gene for improvement of heat tolerance in chickens.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3291-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Liver transcriptome response to hyperthermic stress in three distinct chicken lines

et al. 2016

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“…Through genome resequencing, more than 7,000,000 single nucleotide polymorphisms (SNP) were identified across domestic and wild chickens6. To date, high density SNP panels (60 K and 600 K SNP-Chip) have been successfully used to study distinct natural and experimental chicken populations, resulting in identification of many quantitative trait loci (QTL) and candidate genes for body weight789. In our previous study, using a 60 K SNP-Chip and an intercross of indigenous chickens and commercial broilers, a 3 Mb region on chromosome 1 was identified with a strong association with body weight10.…”

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A short insertion mutation disrupts genesis of miR-16 and causes increased body weight in domesticated chicken

Jia

Lin

Nie

et al. 2016

Sci Rep

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Body weight is one of the most important quantitative traits with high heritability in chicken. We previously mapped a quantitative trait locus (QTL) for body weight by genome-wide association study (GWAS) in an F2 chicken resource population. To identify the causal mutations linked to this QTL, expression profiles were determined on livers of high-weight and low-weight chicken lines by microarray. Combining the expression pattern with SNP effects by GWAS, miR-16 was identified as the most likely potential candidate with a 3.8-fold decrease in high-weight lines. Re-sequencing revealed that a 54-bp insertion mutation in the upstream region of miR-15a-16 displayed high allele frequencies in high-weight commercial broiler line. This mutation resulted in lower miR-16 expression by introducing three novel splicing sites instead of the missing 5′ terminal splicing of mature miR-16. Elevating miR-16 significantly inhibited DF-1 chicken embryo cell proliferation, consistent with a role in suppression of cellular growth. The 54-bp insertion was significantly associated with increased body weight, bone size and muscle mass. Also, the insertion mutation tended towards fixation in commercial broilers (Fst > 0.4). Our findings revealed a novel causative mutation for body weight regulation that aids our basic understanding of growth regulation in birds.

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Water in Livestock – Biological Role and Global Perspective on Water Demand and Supply Chains

Siwek

Sławińska

Dunisławska

2021

Biologically-Inspired Systems

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Identification of quantitative trait loci for body temperature, body weight, breast yield, and digestibility in an advanced intercross line of chickens under heat stress

Cited by 71 publications

References 64 publications

Liver transcriptome response to hyperthermic stress in three distinct chicken lines

Liver transcriptome response to hyperthermic stress in three distinct chicken lines

A short insertion mutation disrupts genesis of miR-16 and causes increased body weight in domesticated chicken

Water in Livestock – Biological Role and Global Perspective on Water Demand and Supply Chains

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