Identification of quantitative trait loci governing subgynoecy in cucumber

Win, Khin Thanda; Zhang, Chunying; Silva, Renato Rodrigues; Lee, Jeong‐Hwan; Kim, Young-Cheon; Lee, Sanghyeob

doi:10.1007/s00122-019-03295-3

Cited by 26 publications

(14 citation statements)

References 64 publications

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“…BSA-seq has been successfully applied for the mapping of important agronomical traits in many crops such as rice (Abe et al, 2012; Takagi et al, 2013; Yang et al, 2013; Sun et al, 2018), lettuce (Huo et al, 2016), potato (Kaminski et al, 2016), soybean (Song et al, 2017), broccoli (Shu et al, 2018; Branham and Farnham, 2019) or sorghum (Han et al, 2015). Moreover, in cucurbits BSA-seq has enabled the identification of candidate genes for dwarfism (Dong et al, 2018), yellow skin (Dou et al, 2018) and light rind color (Oren et al, 2019) in watermelon; mapping flavor traits in melon (Zhang et al, 2016); or the identification of candidate genes for flesh thickness (Xu et al, 2015), aphid resistance (Liang et al, 2016), early flowering QTL (Lu et al, 2014), two major QTLs for downy mildew resistance (Win et al, 2017), and three major QTLs conferring subgynoecy (Win et al, 2019) in cucumber. In the present study, through the use of a BSA-seq strategy, the CsCvy-1 locus has been successfully mapped to a region of 2.9 Mb in chromosome 5, whereas no other regions of the genome exhibited significant association with the resistance.…”

Section: Discussionmentioning

confidence: 99%

Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Cucumis sativus L.) by Using BSA-seq Analysis

Pujol

Alexiou

Fontaine³

et al. 2019

Front. Plant Sci.

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Cucumber vein yellowing virus (CVYV) causes severe yield losses in cucurbit crops across Mediterranean countries. The control of this virus is based on cultural practices to prevent the presence of its vector (Bemisia tabaci) and breeding for natural resistance, which requires the identification of the loci involved and the development of molecular markers for linkage analysis. In this work, we mapped a monogenic locus for resistance to CVYV in cucumber by using a Bulked Segregant Analysis (BSA) strategy coupled with whole-genome resequencing. We phenotyped 135 F3 families from a segregating population between a susceptible pickling cucumber and a resistant Long Dutch type cucumber for CVYV resistance. Phenotypic analysis determined the monogenic and incomplete dominance inheritance of the resistance. We named the locus CsCvy-1. For mapping this locus, 15 resistant and 15 susceptible homozygous F2 individuals were selected for whole genome resequencing. By using a customized bioinformatics pipeline, we identified a unique region in chromosome 5 associated to resistance to CVYV, explaining more than 80% of the variability. The resequencing data provided us with additional SNP markers to decrease the interval of CsCvy-1 to 625 kb, containing 24 annotated genes. Markers flanking CsCvy-1 in a 5.3 cM interval were developed for marker-assisted selection (MAS) in breeding programs and will be useful for the identification of the target gene in future studies.

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Section: Discussionmentioning

confidence: 99%

Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Cucumis sativus L.) by Using BSA-seq Analysis

Pujol

Alexiou

Fontaine³

et al. 2019

Front. Plant Sci.

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“…In a segregating population derived from the cross between S-2-98 (SubG) and M95 (M), 4 QTL, Sgy3.1 , Sgy4.1 , Sgy6.1 , and Sgy6.1 , were found to regulate PFF with Sgy3.1 having the strongest effect (PVE = 54.6%) 39 . In another study, Win et al confirmed the major-effect QTL Sgy3.1 , and identified two additional QTL, Sgy1.1 and Sgy1.2 , which are able to increase, and decrease PFF, respectively 40 (Supplementary File 3). A gene for the GA20-oxidase was proposed to be the candidate gene for the dominantly inherited Sgy3.1 locus 40 .…”

Section: Genes and Qtl For Reproductive Developmentmentioning

confidence: 92%

“…For example, mutations in CsACO2 ( a-1 ) for the 1-aminocyclopropane-1-carboxylate oxidase result in androecy 38 . A major-effect QTL, Sgy3.1 , controls F locus-independent high percentage of female flowers on monoecious plants 39,40 (also see below). These diverse sex-determination genes provide opportunities to fine tune sex expression for cucumber production.…”

Section: Genes and Qtl For Reproductive Developmentmentioning

confidence: 99%

Molecularly tagged genes and quantitative trait loci in cucumber with recommendations for QTL nomenclature

Wang

et al. 2020

Hortic Res

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Cucumber, Cucumis sativus L. (2n = 2x = 14), is an important vegetable crop worldwide. It was the first specialty crop with a publicly available draft genome. Its relatively small, diploid genome, short life cycle, and self-compatible mating system offers advantages for genetic studies. In recent years, significant progress has been made in molecular mapping, and identification of genes and QTL responsible for key phenotypic traits, but a systematic review of the work is lacking. Here, we conducted an extensive literature review on mutants, genes and QTL that have been molecularly mapped or characterized in cucumber. We documented 81 simply inherited trait genes or major-effect QTL that have been cloned or fine mapped. For each gene, detailed information was compiled including chromosome locations, allelic variants and associated polymorphisms, predicted functions, and diagnostic markers that could be used for marker-assisted selection in cucumber breeding. We also documented 322 QTL for 42 quantitative traits, including 109 for disease resistances against seven pathogens. By alignment of these QTL on the latest version of cucumber draft genomes, consensus QTL across multiple studies were inferred, which provided insights into heritable correlations among different traits. Through collaborative efforts among public and private cucumber researchers, we identified 130 quantitative traits and developed a set of recommendations for QTL nomenclature in cucumber. This is the first attempt to systematically summarize, analyze and inventory cucumber mutants, cloned or mapped genes and QTL, which should be a useful resource for the cucurbit research community.

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“…Kubicki (1969b) also described an ac celerato r gene ( acr 1 ), conferring continuous nodes with female flowers in monoecious lines. In subgynoecious cucumber lines, a consistent major quantitative trait locus, which mainly increased the degree of femaleness, was identified on chromosome 3 ( sg3.1 ) from two independent studies (Ji et al, 2016; Win et al, 2019). The relationship between acr 1 or In-F and the genes should be clarified in future studies.…”

Section: Other Sex-type Related Loci Identified Via Genetic Analysismentioning

confidence: 99%

Gene Interactions Regulating Sex Determination in Cucurbits

Sheng

Niu

et al. 2019

Front. Plant Sci.

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The family Cucurbitaceae includes many economically important crops, such as cucumber (Cucumis sativus), melon (Cucumis melo), watermelon (Citrullus lanatus), and zucchini (Cucurbita pepo), which share homologous gene pathways that control similar phenotypes. Sex determination is a research hotspot associated with yield and quality, and the genes involved are highly orthologous and conserved in cucurbits. In the field, six normal sex types have been categorized according to the distribution of female, male, or bisexual flowers in a given plant. To date, five orthologous genes involved in sex determination have been cloned, and their various combinations and expression patterns can explain all the identified sex types. In addition to genetic mechanisms, ethylene controls sex expression in this family. Two ethylene signaling components have been identified recently, which will help us to explore the ethylene signaling-mediated interactions among sex-related genes. This review discusses recent advances relating to the mechanism of sex determination in cucurbits and the prospects for research in this area.

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Identification of quantitative trait loci governing subgynoecy in cucumber

Cited by 26 publications

References 64 publications

Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Cucumis sativus L.) by Using BSA-seq Analysis

Mapping Cucumber Vein Yellowing Virus Resistance in Cucumber (Cucumis sativus L.) by Using BSA-seq Analysis

Molecularly tagged genes and quantitative trait loci in cucumber with recommendations for QTL nomenclature

Gene Interactions Regulating Sex Determination in Cucurbits

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