Identification of quantitative trait loci associated with salt tolerance during seedling growth in soybean (Glycine max L.)

Abstract

Salt stress is an important factor affecting the growth and development of soybean. The inheritance and expression of traits associated with salt tolerance during the seedling stage are complex. The present study was conducted to identify quantitative trait loci (QTLs) associated with salt tolerance during seedling growth in soybean. Field and greenhouse experiments were conducted to evaluate 184 recombinant inbred lines (RILs) derived from a cross between Kefeng No. 1 and Nannong1138-2 for salt tolerance and QTLs that are associated with salt tolerance. The molecular map of this RIL population, covering 2625.9 cM of the genome, converged into 24 linkage groups and consisted of 221 SSR markers and 1 disease-resistant gene (Rsc-7). QTL mapping was conducted using WinQTLCart. Eight putative QTLs significantly associated with salt tolerance were identified. One QTL was identified both in field and greenhouse experiments. In the field, salt tolerance was assessed (tolerance rating, TR) visually on a 0 (death) to 5 (unaffected by salt stress) scale. Three QTLs were detected on two linkage groups explaining 7.1–19.7% of the total phenotypic variance for salt tolerance. In the greenhouse, plant survival days (PSD) and percentage of plant survival (PPS) under salt stress were measured. Six QTLs were detected on six linkage groups, and explained 7.8–19.2% of total phenotypic variation for salt tolerance. A major QTL was identified between markers Sat_164 and Sat_358 on linkage group G in both the field and greenhouse. This QTL qppsN.1 was identified in the same location as a salt tolerance QTL previously reported in soybean. The detection of new QTLs will provide important information for marker-assisted selection (MAS) and further genetic studies on salt tolerance in soybean.

Identification and validation of a major QTL for salt tolerance in soybean

“…SSR marker Satt255 is null in the S-100 and Lee soybean cultivars investigate the performance of the QTL effect of interest in different genetic backgrounds. Comparison of salt tolerance performance of the three sets of NILs showed that the sensitive line of NILs25 had a higher salt tolerance than that of the NILs18 and NILs72, suggesting that there might be another gene that affects or interacts with the salt tolerance gene in linkage group N. Chen et al (2008) detected salttolerant QTLs in several linkage groups, such as linkage groups G, M, and K. In addition, Lee et al (2009) reported a new salt tolerant gene, which is different from the gene in S-100. However, they did not identify the genomic position of the new salt tolerant gene.…”

“…In addition, they also indicated that the salt tolerance confers a large dominant effect over salt sensitivity. Chen et al (2008) evaluated the salt tolerance of 184 F 7:11 RILs derived from a cross between a salttolerant soybean cultivar Kefeng No. 1 and a saltsensitive soybean cultivar Nannong1138-2.…”

Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

“…Several independent studies consistently verified a major quantitative trait locus (QTL) on Chromosome 3 for soybean tolerance to NaCl stress (Chen et al, 2008; Hamwieh and Xu, 2008; Hamwieh et al, 2011; Ha et al, 2013). A cation/H + exchanger family gene, GmCHX1/GmSALT3/Ncl/ ( Glyma03g32900 ), was identified in this genomic region, which regulated Na + , K + , and Cl - homeostasis in the shoot of soybean under NaCl stress, and the functional allele of this gene could improve soybean salt tolerance and yield under salinity stress (Guan et al, 2014; Qi et al, 2014; Tuyen et al, 2016).…”

Salinity Tolerance of Super-Nodulating Soybean Genotype En-b0-1

“…QTLs conditioning salt tolerance in soybean have been identified using recombinant inbred lines (RIL) derived from crosses between salt-tolerant and -susceptible genotypes, (Lee et al, 2004;Chen et al, 2008;Hamwieh and Xu, 2008;Hamwieh et al, 2011;Xu and Tuyen, 2012). A gene conditioning supernodulation has been identified (Arai et al, 2005;Kim et al, 2005).…”