Identification of RAPD markers linked to the rust (Uromyces fabae) resistance gene in pea (Pisum sativum)

Abstract

Two RAPD markers linked to gene for resistance (assayed as pustule number cm −2 leaf area) to rust [Uromyces fabae (Pers.) de Bary] in pea (Pisum sativum L.) were identified using a mapping population of 31 BC 1 F 1 [HUVP 1 (HUVP 1 × FC 1] plants, FC 1 being the resistant parent. The analysis of genetics of rust resistance was based on the parents, generations. Rust resistance in pea is of non-hypersensitive type; it appeared to be governed by a single partially dominant gene for which symbol Ruf is proposed. Further, this trait seems to be affected by some polygenes in addition to the proposed oligogene Ruf. A total of 614 decamer primers were used to survey the parental polymorphism with regard to DNA amplification by polymerase chain reaction. The primers that amplified polymorphic bands present in the resistant parent (FC 1) were used for bulked segregant analysis. Those markers that amplified consistently and differentially in the resistant and susceptible bulks were separately tested with the 31 BC 1 F 1 individuals. Two RAPD makers, viz., SC10-82 360 (primer, GCCGTGAAGT), and SCRI-71 1000 (primer, GTGGCGTAGT), flanking the rust resistance gene (Ruf) with a distance of 10.8 cM (0.097 rF and LOD of 5.05) and 24.5 cM (0.194 rF and a LOD of 2.72), respectively, were identified. These RAPD markers were not close enough to Ruf to allow a dependable maker-assisted selection for rust resistance. However, if the two makers flanking Ruf were used together, the effectiveness of MAS would be improved considerably.

Resistance to rusts (Uromyces pisi and U. viciae-fabae) in pea

“…Many efforts have been made to identify sources of resistance in pea against U. viciae-fabae (Pal et al 1980;Xue & Warkentin 2001;Singh et al 2004;Vijayalakshmi et al 2005;Kushwaha et al 2006). These studies revealed that the majority of the studied pea genotypes were susceptible, though genotypic differences in the rust intensity described as of slow rusting type have been reported (Kumar et al 1994;Chand et al 2006) (Table 1).…”

“…The dominant nature of partial resistance against faba bean rust U. viciae-fabae in pea, recorded as reduced infection frequency, has been justified as the expression of a single major gene, for which the symbol Ruf was proposed (Vijayalakshmi et al 2005), but the authors also presented some evidence suggesting involvement of some polygenes as well. Further, none of the pea genotypes has been reported to be free from U. viciae-fabae infection (Singh & Sivastava 1985;Chand et al 2006) suggesting a polygenic type of resistance or based on incomplete gene expression.…”

“…More recently, Rai et al (2011) suggested that the Ruf gene proposed by Vijayalakshmi et al (2005) be now redesigned as Qruf to signify the quantitative nature of its action and detected another minor quantitative trait loci (QTL) (named Qruf1). Both QTLs were located on LGVII.…”

“…U. fabae (Pers.) de Bary] (Pal et al 1980;Xue & Warkentin 2001;Singh et al 2004;Vijayalakshmi et al 2005;Kushwaha et al 2006) or by U. pisi [(Pers. ) Wint.]…”

Disease resistance in pea (Pisum sativum L.) types for autumn sowings in Mediterranean environments - a review

“…Several sources of incomplete resistance against U. viciae-fabae have been reported (Pal et al 1980;Xue & Warketin 2001;Vijayalakshmi et al 2005;Chand et al 2006;Kushwaha et al 2006). A single major gene (Ruf) has been reported as responsible for this partial resistance.…”