Lipids in sewage sludge are considered to be high-class raw materials for biodiesel or other valuable products. We profiled the characteristics and assessed the reutilization potential of lipids from different sludge sources, including sludge from the primary sedimentation tank (PST sludge) and sludge from the secondary sedimentation tank in a conventional activated sludge system (CAS sludge), as well as sludge from ultrashort-sludge retention time (SRT) activated sludge systems with different SRTs (USAS sludge, with SRTs of 0.5, 1.0, 2.0, 3.0 and 4.0 d). The results showed that the lipids in the sludges were mainly composed of cellular lipids, free fatty acids (FFAs), wax and gum. The highest lipid content was found in the PST sludge (156.8 ± 11.9 mg/g, dry basis), followed by the USAS sludges (67.9 ± 11.0-132.2 ± 11.8 mg/g) and the CAS sludge (46.0 ± 16.5 mg/g). Lipid species such as Cer, So, PE, PC, and TG were abundant, comprising over 80% of the cellular lipids in the sludges. With higher lipid contents, the PST sludge and USAS sludge (0.5 d SRT) were suggested to have a higher reutilization potential for use in producing biodiesel. In addition, the CAS sludge was promising for resource reutilization and energy recovery due to the large amount of excess sludge. By the end of June 2017, over 3,700 wastewater treatment plants (WWTPs) 1 had been built and operated in China, and a large amount of sludge was produced each year. Based on a report from the China Statistical Yearbook on Environment (2018), the yield of municipal sludge with 80% moisture content increased from 33.18 billion tons in 2008 to 49.24 billion tons in 2017 1. Thus, sludge treatment has become an urgent issue in China, and the main treatment method is landfilling. However, sludges from the primary and secondary sedimentation tanks in WWTPs contain a large amount of organic matter 2 , which has the potential for resource and energy recovery. In particular, the concentration of lipids in sludge has been reported to be high, accounting for approximately 20% of the organic matter 3 , and thus, lipids are considered to be a potential feedstock for use in diesel production 4-8. The lipids in municipal sewage sludge include cellular lipids, free fatty acids, wax and gum, the contents and diesel production potential of which are affected by the sludge source. It has been reported that the biodiesel yield of sludge from the primary sedimentation tank (PST) is much higher than that of sludge from the secondary sedimentation tank in the conventional activated sludge (CAS) process due to substrate utilization by the metabolism of microorganisms in the bioreactor 9. One possible approach to achieve resource recovery from sludge in WWTPs is to prevent the substrates from being utilized by microorganisms by reducing the sludge retention time (SRT) in the CAS process. Recently, an ultrashort-SRT activated sludge (USAS) process was developed for organic recovery; this process originates from the A stage of the adsorption-biodegradation (AB) process, where th...