Identification of regulators of the early stage of viral hemorrhagic septicemia virus infection during curcumin treatment

Jeong, Eun-Hye; Vaidya, Bipin; Cho, Se-Young; Park, Myoung-Ae; Kaewintajuk, Kusuma; Kim, Seok Ryel; Oh, Myung‐Joo; Choi, Jong‐Soon; Kwon, Joseph; Kim, Duwoon

doi:10.1016/j.fsi.2015.03.042

Cited by 42 publications

(32 citation statements)

References 59 publications

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“…Proteins were extracted as previously described [51]. The protein was quantified using a Detergent Compatible Protein Assay Kit (Bio-Rad).…”

Section: Protein Extraction and Tube-gel Protein Digestionmentioning

confidence: 99%

“…The protein was quantified using a Detergent Compatible Protein Assay Kit (Bio-Rad). A tube-gel digestion protocol was adopted as described previously [51]. MCF-7 cells were cultured for 96 h in estrogen-free medium and treated with 20 µg/mL of SJW extract, 10 µM HF, or 10 µM 17β-E2 in the presence and absence of ICI 182,780.…”

Section: Protein Extraction and Tube-gel Protein Digestionmentioning

confidence: 99%

“…Tryptic peptide mixtures were separated using nano-ACQUITY ultra-performance liquid chromatograph (UPLC) equipped with a Synapt G2-Si HDMS System (Waters Corp.), a previously described method with optimization of the mobile phase system [51]. A gradient elution program was conducted for chromatographic separation with mobile phase A (0.1% formic acid in water) and mobile phase B (0.1 % formic acid in acetonitrile) as follows: 97% mobile phase A initially, 90% mobile phase A for 3 min, 65 % mobile phase A for 150 min, gradual decrease to 20 % mobile phase A over 160 min, and a sharp increase to 97 % mobile phase A for the last 10 min.…”

Section: Nano-uplc-hdms Ementioning

confidence: 99%

“…For identification of proteins, MS spectra of peptides were aligned using Progenesis QI for Proteomics (QIP) version 2.0 (Nonlinear Dynamics), a previously adopted method with modifications in criteria for protein identification [51], and the spectra were matched to human proteins using the International Protein Index (IPI) human database (v.3.87). The criteria for protein identification were set as follows: ≥ 3 fragment per peptide, ≥ 7 fragments per protein, and ≥ 2 peptides per protein.…”

Section: Nano-uplc-hdms Ementioning

confidence: 99%

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Estrogenic Activity of Hyperforin in MCF-7 Human Breast Cancer Cells Transfected with Estrogen Receptor

Kwon

Cho

et al. 2016

Planta Med

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Hyperforin, a major active compound of St.?John?s wort extract, affects estrogenic activity. In this study, the compound evoked estrogen response element-dependent luciferase activity and cell proliferation in MCF-7 cells. Hyperforin-induced cell proliferation was significantly inhibited by the estrogen receptor antagonist ICI 182,780. These results suggested that hyperforin had estrogenic and cell proliferation activities, which were stimulated via the estrogen receptor. Compared to 17?-estradiol, hyperforin showed significantly lower estrogenic activity and cell proliferation. The mechanism underlying the estrogenic activity of hyperforin was unknown, therefore, in this study, for the first time, the expression and post-translational modification of proteins were determined and compared among control, 17?-estradiol-treated, and hyperforin-treated cells using proteomic techniques. A total of 453 proteins were identified, of which 282 proteins were significantly modulated in hyperforin-treated cells compared to 17?-estradiol-treated cells. Ingenuity pathway analysis also demonstrated that hyperforin treatment induced less cell proliferation than 17?-estradiol by downregulating estrogen receptor 1. Protein network analysis showed that cell proliferation was regulated mainly by cyclin D1 and extracellular signal-regulated kinases. In conclusion, although, hyperforin exhibited lower estrogenic activity than 17?-estradiol, the compound induced lower levels of cancer cell proliferation in vitro.

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“…Proteins were extracted as previously described [51]. The protein was quantified using a Detergent Compatible Protein Assay Kit (Bio-Rad).…”

Section: Protein Extraction and Tube-gel Protein Digestionmentioning

confidence: 99%

Section: Protein Extraction and Tube-gel Protein Digestionmentioning

confidence: 99%

Section: Nano-uplc-hdms Ementioning

confidence: 99%

Section: Nano-uplc-hdms Ementioning

confidence: 99%

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Estrogenic Activity of Hyperforin in MCF-7 Human Breast Cancer Cells Transfected with Estrogen Receptor

Kwon

Cho

et al. 2016

Planta Med

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“…curcumin, a polyphenol, is the major active component of turmeric. Various studies have documented the therapeutic benefits of curcumin in managing various disorders due to its anti-inflammatory, anti-oxidant and anti-viral properties (5)(6)(7). curcumin was previously reported to effectively control the occurrence and development of asthma by reducing inflammation, inhibiting airway remodeling and maintaining structural integrity (8).…”

Section: Introductionmentioning

confidence: 99%

Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway

et al. 2018

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Excessive mucin production in the airway may contribute to airway inflammatory diseases. Curcumin has been reported to prevent mucin 5AC (MUC5AC) production in human airway epithelial cells; however, the molecular targets of curcumin involved in regulating MUC5AC expression have remained elusive. The present study aimed to elucidate the molecular mechanisms by which curcumin regulates MUC5AC production, utilizing the NCI‑H292 human airway epithelial cell line featuring MUC5AC hypersecretion. Curcumin was able to counteract the endothelial growth factor (EGF)‑stimulated mRNA and protein expression of MUC5AC. In addition, curcumin treatment prevented EGF‑induced phosphorylation of EGF receptor (EGFR) as well as the downstream AKT and signal transducer and activator of transcription 3 (STAT3), while inhibition of PI3K and STAT3 signaling significantly attenuated the expression of MUC5AC that was induced by EGF. Furthermore, EGF‑induced increases in the levels of phosphorylated STAT3 in the nuclear fraction were inhibited by curcumin and PI3K inhibitors. In addition, treatment with curcumin significantly decreased MUC5AC and EGFR expression in a time‑dependent manner under basal conditions. These results demonstrated that curcumin inhibited MUC5AC protein expression in NCI‑H292 cells under basal conditions as well under EGF stimulation. This inhibition was accompanied by decreased activation of the EGFR/AKT/STAT3 pathway and reduced EGFR expression, which indicated that curcumin may have a dual role in interfering with the EGFR signaling pathway and inhibiting mucin expression in human airway epithelial cells.

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Medicinal Plants in Controlling and Treatment of Diseases Caused by Viral Hemorrhagic Septicemia Virus (VHSV)

Gurjar

2023

Reference Series in Phytochemistry

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Identification of regulators of the early stage of viral hemorrhagic septicemia virus infection during curcumin treatment

Cited by 42 publications

References 59 publications

Estrogenic Activity of Hyperforin in MCF-7 Human Breast Cancer Cells Transfected with Estrogen Receptor

Estrogenic Activity of Hyperforin in MCF-7 Human Breast Cancer Cells Transfected with Estrogen Receptor

Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway

Medicinal Plants in Controlling and Treatment of Diseases Caused by Viral Hemorrhagic Septicemia Virus (VHSV)

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