Identification of regulatory elements in the human adipose most abundant gene transcript-1 (apM-1) promoter: role of SP1/SP3 and TNF-? as regulatory pathways

Barth, N.; Langmann, Thomas; Schlmerich, J.; Schmitz, Gerd; Sch�ffler, A.

doi:10.1007/s00125-003-1278-2

Cited by 19 publications

(20 citation statements)

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“…Both binding sites are involved in adipocyte differentiation (27,28). It has been shown that the first intron of the human ADIPOQ gene contains a gene expression enhancer element, which responds to C/EBP a, but not to C/EBP b (29).…”

Section: Discussionmentioning

confidence: 99%

Association of genetic variants in the adiponectin gene with adiponectin level and hypertension in Hong Kong Chinese

Ong¹,

Li²,

Tso

et al. 2010

European Journal of Endocrinology

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Objective: Low plasma adiponectin level can predict the development of hypertension after 5 years in our population. We therefore investigated whether single-nucleotide polymorphisms (SNPs) in the adiponectin gene influenced plasma adiponectin level and whether they were associated with hypertension. Design and methods: We genotyped 14 tagging SNPs in 1616 subjects with persistent normotensive or hypertensive status during a 6.4-year follow-up period in the Hong Kong Cardiovascular Risk Factor Prevalence Study-2 (CRISPS-2). Plasma adiponectin level was measured in 1385 subjects using in-house sandwich ELISA. Results: The minor G allele of the SNP rs266729 was significantly associated with higher odds of hypertension (odds ratio (95% confidence interval)Z1.49 (1.13-1.95), PZ0.0044) after adjusting for covariates. In stepwise multiple logistic regression, this SNP (PZ0.006) was a significant independent factor of hypertension, together with age (P!0.001), body mass index (P!0.001), triglycerides (PZ0.021), and insulin resistance index (P!0.001). Among the 14 SNPs, rs266729 (bZK0.067, PZ0.0037), K10677COT (bZ0.069, PZ0.0027), rs182052 (bZK0.097, P!0.0001), and rs12495941 (bZ0.103, P!0.0001) were significantly associated with adiponectin level after adjusting for covariates. No significant sex interaction was found for the associations of SNPs with hypertension and adiponectin level. Similar results were obtained in haplotype analysis. Conclusion: In our population, genetic variants in the adiponectin gene influenced plasma adiponectin levels, and one of them was associated with hypertension. This study has provided further evidence for a role of adiponectin in the development of hypertension.

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Section: Discussionmentioning

confidence: 99%

Association of genetic variants in the adiponectin gene with adiponectin level and hypertension in Hong Kong Chinese

Ong¹,

Li²,

Tso

et al. 2010

European Journal of Endocrinology

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“…This would be in addition to demonstrated actions to drive adipocyte differentiation (41,42). However, there is conflicting data about the importance of PPAR-␥ in control of apM-1 gene transcription (43)(44)(45). The failure of metformin treatment to alter adiponectin expression further suggests that it is not changes in circulating glucose or insulin levels, but rather direct actions on the adipocyte that are driving the regulation of adiponectin, as we found that metformin treatment also did not effect glucose transport or insulin signaling in adipocytes (22).…”

Section: Discussionmentioning

confidence: 99%

Modulation of Circulating and Adipose Tissue Adiponectin Levels by Antidiabetic Therapy

et al. 2003

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The relationship between insulin action and control of the adipocyte-derived factor adiponectin was studied in age-and weight-matched obese individuals with type 2 diabetes failing sulfonylurea therapy. After initial metabolic characterization, subjects were randomized to troglitazone or metformin treatment groups; all subjects received glyburide (10 mg BID) as well. Treatment was continued for 3 months. The extent of glycemic control after treatment was similar in both groups. However, the increase in maximal insulin-stimulated glucose disposal rate was greater following troglitazone therapy (؉44%) compared with metformin treatment (؉20%). Troglitazone treatment increased serum adiponectin levels nearly threefold. There was no change in serum adiponectin with metformin treatment. A positive correlation was found between increases in wholebody glucose disposal rates and serum adiponectin levels after troglitazone; no such relationship was seen with metformin. The adiponectin protein content of subcutaneous abdominal adipocytes was increased following troglitazone treatment and unchanged after metformin. Adiponectin release from adipocytes was also augmented with troglitazone treatment. Adiponectin was present in adipocytes and plasma in several multimeric forms; a trimer was the major form secreted from adipocytes. These results indicate that increases in adiponectin content and secretion are associated with improved insulin action but are not directly related to glycemic control. Modulation of adipocyte function, including upregulation of adiponectin synthesis and secretion, may be an important mechanism by which thiazolidinediones influence insulin action. Diabetes

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“…The promoter activity of the adiponectin gene has been shown to be markedly enhanced by the TZDs (14), although the presence of a functional PPAR-␥ response element in the adiponectin gene remains controversial (46,47). The induction of adiponectin in fact might be caused by secondary effects involving other PPARinducible genes and not by specific activation of the PPAR response elements (48). In support of an important role for PPAR-␥ in regulation of adiponectin synthesis, circulating adiponectin levels were found by Combs et al (17) to be suppressed fivefold in patients with severe insulin resistance in association with dominant-negative PPAR-␥ mutations.…”

Section: Metabolic Roles Of Adiponectinmentioning

confidence: 99%

Adiponectin: More Than Just Another Fat Cell Hormone?

et al. 2003

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Identification of regulatory elements in the human adipose most abundant gene transcript-1 (apM-1) promoter: role of SP1/SP3 and TNF-? as regulatory pathways

Cited by 19 publications

References 0 publications

Association of genetic variants in the adiponectin gene with adiponectin level and hypertension in Hong Kong Chinese

Association of genetic variants in the adiponectin gene with adiponectin level and hypertension in Hong Kong Chinese

Modulation of Circulating and Adipose Tissue Adiponectin Levels by Antidiabetic Therapy

Adiponectin: More Than Just Another Fat Cell Hormone?

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