Identification of Repressor Element 1 in Secretin/PACAP/VIP Genes

Lee, Leo Tsz On; Lee, Vien H.Y.; Yuan, Paula Y.; Chow, Billy K. C.

doi:10.1196/annals.1317.050

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…The 5Ј-flanking region contains two neural-restrictive silencer-like elements 1 and 2, which might be involved in neuron-specific PACAP gene expression (Fig. 4) Lee et al, 2006).…”

Section: The Pituitary Adenylate Cyclase-activating Polypeptide Genementioning

confidence: 99%

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

Vaudry

Falluel‐Morel

Bourgault

et al. 2009

Pharmacological Reviews

1,172

1,610

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“…The 5Ј-flanking region contains two neural-restrictive silencer-like elements 1 and 2, which might be involved in neuron-specific PACAP gene expression (Fig. 4) Lee et al, 2006).…”

Section: The Pituitary Adenylate Cyclase-activating Polypeptide Genementioning

confidence: 99%

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

Vaudry

Falluel‐Morel

Bourgault

et al. 2009

Pharmacological Reviews

1,172

1,610

View full text Add to dashboard Cite

“…3B). 52 NRSE is known to be the binding site for neuron‐restrictive silencer factor (NRSF), and it functions as a transcriptional repressor that downregulates the expression of neuron‐specific genes in non‐neuronal tissues 53,54 . It was also reported that NRSF interacts with Sp1 through its C‐terminal repressor domain and inhibits Sp1‐dependent activation in nonneuronal and non–beta cells 55 .…”

Section: Structure and Regulation Of The Human Secretin Receptor (Hsrmentioning

confidence: 99%

Extragastrointestinal functions and transcriptional regulation of secretin and secretin receptors

Yuan

Lee

et al. 2011

Annals of the New York Academy of Sciences

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The functional role of secretin outside the gastrointestinal system has been a mystery to endocrinologists for many years. With the availability of secretin- and secretin receptor-knockout models and the advances of electrophysiological and immunohistochemical techniques, we are now in a good position to explore the functions of secretin outside the gastrointestinal system. There is growing evidence to support that secretin works pleiotropically at multiple levels in our body. In this paper, we review the recent findings regarding the physiological effects of secretin on extragastrointestinal sites including the cerebellum, the hippocampus, and the hypothalamus-pituitary-kidney axis. To understand the mechanisms underlying the site-specific expression of secretin and secretin receptors in the central nervous system, we also discuss the transcriptional regulation of secretin and secretin receptor genes in neuronal cell lines.

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“…Both Sin3 and CoREST interact with histone deacetylase containing complex to deacetylate core histone proteins, and as a consequence, they work together to silence target genes by forming condensed chromatin structures and mediates developmental stage-specific gene expression [14,15,16]. An in silico analysis indicates that all members of the secretin receptor family contain at least one putative NRSE-like motif in their 5' flanking regions [17].…”

Section: Introductionmentioning

confidence: 99%

Neuron-restrictive silencer factor functions to suppress Sp1-mediated transactivation of human secretin receptor gene

Yuan

Chow

Lee

et al. 2013

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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In the present study, a functional neuron restrictive silencer element (NRSE) was initially identified in the 5' flanking region (-83 to -67, relative to ATG) of human secretin receptor (hSCTR) gene by promoter assays coupled with scanning mutation analyses. The interaction of neuron restrictive silencer factor (NRSF) with this motif was later indicated via gel mobility shift and ChIP assays. The silencing activity of NRSF was confirmed by over-expression and also by shRNA knock-down of endogenous NRSF. These studies showed an inverse relationship between the expression levels of NRSF and hSCTR in the cells. As hSCTR gene was previously shown to be controlled by two GC-boxes which are regulated by the ratio of Sp1 to Sp3, in the present study, the functional interactions of NRSF and Sp proteins to regulate hSCTR gene was investigated. By co-immunoprecipitation assays, we found that NRSF could be co-precipitated with Sp1 as well as Sp3 in PANC-1 cells. Interestingly, co-expressions of these factors showed that NRSF could suppress Sp1-mediated, but not Sp3-mediated, transactivation of hSCTR. Taken together, we propose here that the down-regulatory effects of NRSF on hSCTR gene expression are mediated via its suppression on Sp1-mediated transactivation.

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Identification of Repressor Element 1 in Secretin/PACAP/VIP Genes

Cited by 5 publications

References 21 publications

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

Extragastrointestinal functions and transcriptional regulation of secretin and secretin receptors

Neuron-restrictive silencer factor functions to suppress Sp1-mediated transactivation of human secretin receptor gene

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