Identification of respiratory pathogen Bordetella Pertussis using integrated microfluidic chip technology

Prakash, A. Ranjit; Rosa, Carlos de; Fox, Julie D.; Kaler, K.V.I.S.

doi:10.1007/s10404-007-0195-y

Cited by 19 publications

(19 citation statements)

References 34 publications

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“…Capillary electrophoresis is a powerful technique for separating and analyzing samples in a short time (Lin et al 2008;Prakash et al 2008). However, conventional CE devices rely on fabricating real microchannels in glass, PMMA or PDMS for delivering and separating samples (Cai and Neyer 2010;Hong et al 2010;Hug et al 2006;Hupert et al 2007;Zhu and Petkovic-Duran 2010).…”

Section: Introductionmentioning

confidence: 99%

Electrophoresis separation and electrochemical detection on a novel thread-based microfluidic device

Wei

Fu²,

Lin

2012

Microfluid Nanofluid

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This paper describes a thread-based microfluidic system for rapid and low-cost electrophoresis separation and electrochemical (EC) detection of ion samples. Instead of using liquid channel for sample separation, thin polyester threads of various diameters are used as the routes for separating the samples with electrophoresis. Hot-pressed PMMA chip with protruding sleeper structures are adopted to set up the polyester threads and for electrochemical detection of the ion samples on the thread. Plasma treatment greatly improves the wetability of thin threads and surface quality of the threads. The measured electrical currents on plasma treated threads are 10 times greater than the threads without treatment. Results indicate that nice redox signals can be obtained by measuring ferric cyanide salt on the polyester thread. The estimated detection limit for EC sensing of potassium ferricyanide (K 3 Fe(CN) 6 ) is around 6.25 lM using the developed thread-based microfluidic device. Mixed ion samples (Cl -, Br -and I -) and bio-sample are successfully separated and detected using the developed thread-based microfluidic device.

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Section: Introductionmentioning

confidence: 99%

Electrophoresis separation and electrochemical detection on a novel thread-based microfluidic device

Wei

Fu²,

Lin

2012

Microfluid Nanofluid

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“…The polymerase chain reaction (PCR) technology for DNA amplification was first reported by Kary Mullis (Saiki et al 1985), which has been applied to a diverse range of basic research and application fields. Recently, attention has focused on developing microfluidic-based PCR devices (Nakano et al 1994;Northrup et al 1993), since they offer lower thermal capacitance for rapid thermal cycling , reduced analysis-times, low consumption of sample/reagent, portability, and the potential for high automation and integration of various analytical procedures (Obeid and Christopoulos 2004;Prakash et al 2008b). Generally, microfluidic PCR devices can be divided into two formats (Zhang et al 2006;: microchamber stationary PCR (Beer et al 2007;Belgrader et al 2003;Ottesen et al 2006;Prakash and Kaler 2007), which can achieve PCR functions by adjusting temperature cycling in a stationary microchamber, and continuous-flow PCR (Dorfman et al 2005;Kiss et al 2008;Kopp et al 1998;Park et al 2003;Wang et al 2009), in which the reaction mixture moves through two or three different temperature regions, thus the thermal cycling is obtained.…”

Section: Introductionmentioning

confidence: 99%

Fast identification of foodborne pathogenic viruses using continuous-flow reverse transcription-PCR with fluorescence detection

Zhang

Xing

2010

Microfluid Nanofluid

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Being beneficial from dramatic process in biochemical and micro-electro-mechanical technologies, this study presents an integrated microfluidic system capable of performing a continuous-flow reverse transcription-polymerase chain reaction (RT-PCR) combined with fluorescence microscopy for rapid diagnosis of RNA-based viruses. The device consists of two heated cylinders for heating the different reaction zones of the reverse transcription and the amplification. In the amplification cylinder, bath refrigerating is used for thermal protection of the annealing region, which is heated possibly due to the thermal effects of radiation, conduction, and/or convection from denaturation and extension regions, thus resulting in a space-saving design of the whole device. Detection of the amplified products is performed on-line by a fluorescence microscopy with SybrGreen I, a widely used intercalating dye. In this article, the proposed miniature RT-PCR system is used to amplify and detect two RNA-based viruses (Noroviruses (NVs) and Rotaviruses (RVs)), which are now recognized as the most common etiological agents of acute viral gastroenteritis causing numerous outbreaks worldwide. The experimental data have demonstrated the ability of the presented system to perform a two-step or one-step RT-PCR process. On this device, the NVs and RVs RNA samples were successfully reverse transcribed and amplified within 1 h, and the limit of detection of the RNA concentration was 6.4 9 10 4 copies ll -1 using onestep RT-PCR process. Consequently, the developed microfluidic system can provide a promising platform for fast diagnosis of RNA-based viruses.

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“…Prakash et al . integrated nine independent PCR chambers with one CE separation channel on a single chip for simultaneous amplifi cation and sequential detection © Woodhead Publishing Limited, 2013 of respiratory pathogen Bordetella pertussis (Prakash et al , 2008). Pal et al have developed a more complicated microfl uidic device, integrating two nLscale reactors for sequential PCR and restriction fragment length polymorphism (RFLP) reactions, valves and mixers for microfl uidic control, and a separation channel for miniaturised gel electrophoresis (Pal et al ., 2005).…”

Section: Pathogen/infectious Disease Detectionmentioning

confidence: 99%

Integrated microfluidic systems for genetic analysis

Zhuang

Gan

Liu

2013

Microfluidic Devices for Biomedical Applications

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Identification of respiratory pathogen Bordetella Pertussis using integrated microfluidic chip technology

Cited by 19 publications

References 34 publications

Electrophoresis separation and electrochemical detection on a novel thread-based microfluidic device

Electrophoresis separation and electrochemical detection on a novel thread-based microfluidic device

Fast identification of foodborne pathogenic viruses using continuous-flow reverse transcription-PCR with fluorescence detection

Integrated microfluidic systems for genetic analysis

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