Identification of S-RNase and peroxidase in petunia nectar

Protein and alkaloid patterns of the floral nectar in some solanaceous species

“…Other enzymes including ribonucleases, desoxyribonucleases, a peroxidase, an endochitinase, the presumed fructokinase which can prevent microbial colonization in the nectar of Petunia hybrida; as well as ribonucleases, a peroxidase and a chitinase were identified in the nectar of Nicotiana langsdorffii × Nicotiana sanderae var. LxS8 [40][41].…”

Effect of honey on oxidation, chlorination and nitration by purified equine myeloperoxidase

“…However, according to Kwakman and Zaat [12], catalase has never been identified in honey. Interestingly, peroxidases are among the most abundant proteins in petunia nectar and the level of H 2 O 2 accumulation in tobacco and petunia nectar is inversely related to the level of peroxidase activity in these nectars [13]. Possibly nectar-derived peroxidases rather than catalase might be a cause of variation in H 2 O 2 -neutralizing capacity of different honeys.…”

Analysis of the role of RNS2 and autophagy in the turnover of ribosomal RNA in Arabidopsis thaliana

“…8A). Acid phosphatase is a vacuolar resident enzyme in plant cells 54,55 and has similar activity in WT, rns2 -2, atg9-4, atg5-1, rns2-2atg9-4, and rns2-2atg5-1 vacuoles (Fig. 8B).…”

“…It is very likely that NnSR1 recycles Pi from endogenous rRNA, the main reserve source of cellular Pi. The localization and function of NnSR1 resemble those of S-like RNases, suggesting that after duplication of the S-locus, NnSR1 regained ancestral functions of class I or class II RNases, to adapt to environmental challenges [53,54]. Further research is required to determine whether this RNase is specifically expressed under Pi deficit or, like class I S-like RNases, is also induced under other environmental stresses.…”

“…93 qPCR results were normalized to the vacuole marker enzyme acid phosphatase activity, whish was measured as previously described. 54,94 Three to five biological replicates per genotype were carried out. (A) Twenty-six-day-old Arabidopsis rosettes from WT, Vacuoles were purified from these protoplasts, and checked for quality under the microscope.…”