Identification of salt tolerance-related genes of Lactobacillus plantarum D31 and T9 strains by genomic analysis

Yao, Wenting; Yang, Lianzhi; Shao, Zhigang; Xie, Lu; Chen, Lanming

doi:10.1186/s13213-020-01551-2

Cited by 26 publications

(27 citation statements)

References 62 publications

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“…On the contrary, other studies [ 39 , 40 , 41 ] reported the ability of L. plantarum strains to tolerate high (> 8%) NaCl concentrations. The tolerance to high salt concentrations and the ability to control osmotic stress have been recently confirmed [ 28 ], showing excellent growth performance of L. plantarum strains at 5% and 8% of NaCl. The authors observed that L. plantarum strains are equipped, at genomic level, with a complex molecular regulatory network involving genes associated with salt resistance through the recovery of the intracellular ion balance [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

“…The tolerance to high salt concentrations and the ability to control osmotic stress have been recently confirmed [ 28 ], showing excellent growth performance of L. plantarum strains at 5% and 8% of NaCl. The authors observed that L. plantarum strains are equipped, at genomic level, with a complex molecular regulatory network involving genes associated with salt resistance through the recovery of the intracellular ion balance [ 28 ]. Our study clearly highlights that the growth behavior under stress conditions is strain-specific, especially at 16 °C ( Figure 2 and Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

“…Up to now, different strains were tested for the ability to degrade the oleuropein under optimal growth conditions [ 19 , 26 , 27 ] or using MRS medium added with different salt concentrations [ 16 , 17 , 20 ]. In this context, Yao et al [ 28 ] demonstrated that the tolerance of L. plantarum D31 and T9 strains, to 5% and 8% of salt, is related to the presence of specific salt tolerance-related genes.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives

et al. 2020

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The use of β-glucosidase positive strains, as tailored-starter cultures for table olives fermentation, is a useful biotechnological tool applied to accelerate the debittering process. Nowadays, strains belonging to Lactiplantibacillus plantarum species are selected for their high versatility and tolerance to stress conditions. The present study investigated the effect of different stress factors (pH, temperature and NaCl) on growth and on oleuropein-degrading abilities of selected L. plantarum strains. In addition, the presence of the beta-glucosidase gene was investigated by applying a PCR based approach. Results revealed that, overall, the performances of the tested strains appeared to be robust toward the different stressors. However, the temperature of 16 °C significantly affected the growth performance of the strains both singularly and in combination with other stressing factors since it prolongs the latency phase and reduces the maximum growth rate of strains. Similarly, the oleuropein degradation was mainly affected by the low temperature, especially in presence of low salt content. Despite all strains displayed the ability to reduce the oleuropein content, the beta-glucosidase gene was detected in five out of the nine selected strains, demonstrating that the ability to hydrolyze the oleuropein is not closely related to the presence of beta-glucosidase. Data of the present study suggest that is extremely important to test the technological performances of strains at process conditions in order to achieve a good selection of tailored starter cultures for table olives.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives

et al. 2020

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“…Other genes enriched on the plasmids from NL mainly include chloride channel protein, calcium-transporting ATPase, magnesium transporter, and Na + /H + antiporter (Table S5). These proteins are involved in functions such as acid resistance, salt tolerance, metal ion homeostasis and oxidative stress tolerance [46][47][48], probably suggesting their contribution to adaptation of NL to dynamic environments.…”

Section: Inorganic Ion Transport and Metabolismmentioning

confidence: 99%

Plasmids encode niche-specific traits in Lactobacillaceae

2021

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Species belonging to the family Lactobacillaceae are found in highly diverse environments and play an important role in fermented foods and probiotic products. Many of these species have been individually reported to harbour plasmids that encode important genes. In this study, we performed comparative genomic analysis of publicly available data for 512 plasmids from 282 strains represented by 51 species of this family and correlated the genomic features of plasmids with the ecological niches in which these species are found. Two-thirds of the species had at least one plasmid-harbouring strain. Plasmid abundance and GC content were significantly lower in vertebrate-adapted species as compared to nomadic and free-living species. Hierarchical clustering highlighted the distinct nature of plasmids from the nomadic and free-living species than those from the vertebrate-adapted species. EggNOG-assisted functional annotation revealed that genes associated with transposition, conjugation, DNA repair and recombination, exopolysaccharide production, metal ion transport, toxin–antitoxin system, and stress tolerance were significantly enriched on the plasmids of the nomadic and in some cases nomadic and free-living species. On the other hand, genes related to anaerobic metabolism, ABC transporters and the major facilitator superfamily were overrepresented on the plasmids of the vertebrate-adapted species. These genomic signatures correlate with the comparatively nutrient-depleted, stressful and dynamic environments of nomadic and free-living species and nutrient-rich and anaerobic environments of vertebrate-adapted species. Thus, these results indicate the contribution of the plasmids in the adaptation of lactobacilli to their respective habitats. This study also underlines the potential application of these plasmids in improving the technological and probiotic properties of lactic acid bacteria.

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“…Other genes enriched on the plasmids from NL mainly include chloride channel protein, calcium-transporting ATPase, magnesium transporter, and Na+/H+ antiporter (Table S5). These proteins are involved in functions such as acid resistance, salt tolerance, metal ion homeostasis, and oxidative stress tolerance (46), (47), (48), probably suggesting their contribution to adaptation of NL to the dynamic environments.…”

Section: Resultsmentioning

confidence: 99%

Plasmids encode niche-specific traits inLactobacillaceae

Davray

Deo

Kulkarni

2020

Preprint

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The species of family Lactobacillaceae are found in highly diverse environments and play an important role in fermented foods and probiotic products. Many of these species have been individually reported to harbor plasmids that encode important genes. In this study, we performed comparative genomic analysis of the publically available data of 512 plasmids from 282 strains represented by 51 species of this family and correlated the genomic features of plasmids with the ecological niches in which these species are found. Two-third of the species had at least one plasmid-harboring strain. Plasmid abundance and GC content were significantly lower in the host-adapted species as compared to the nomadic and free-living species. Hierarchical clustering (HCL) highlighted the distinct nature of plasmids from the nomadic and free-living species than those from the host-adapted species. EggNOG assisted functional annotation revealed that genes associated with transposition, conjugation, DNA repair and recombination, exopolysaccharide production, metal ion transport, toxin-antitoxin system, and stress tolerance were significantly enriched on the plasmids of the nomadic and in some cases nomadic and free-living species. On the other hand, genes related to anaerobic metabolism, ABC transporters, and major facilitator superfamily were found to be overrepresented on the plasmids of the host-adapted species. These genomic signatures are correlated to the comparatively nutrient-depleted, stressful and dynamic environments of nomadic and free-living species and nutrient-rich and anaerobic environments of the host-adapted species. Thus, these results indicate the contribution of the plasmids in the adaptation of lactobacilli to the respective habitats. This study also underlines the potential application of these plasmids in improving the technological and probiotic properties of lactic acid bacteria.

show abstract

Identification of salt tolerance-related genes of Lactobacillus plantarum D31 and T9 strains by genomic analysis

Cited by 26 publications

References 62 publications

Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives

Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives

Plasmids encode niche-specific traits in Lactobacillaceae

Plasmids encode niche-specific traits inLactobacillaceae

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