Identification of Schizosaccharomyces Pombe Ird Mutants Resistant to Glucose Suppression and Oxidative Stress

Abstract

BackgroundGlucose both is the favorite carbon and energy source and acts as a hormone that plays a regulated role in many biological processes. Calorie restriction extended lifespan in many organisms, including Schizosaccharomyces pombe, while uptake of high glucose led to undesired results, such as diabetes and aging.Methods and ResultsIn this study, sequence analysis of Schizosaccharomyces pombe ird5 and ird11 mutants was performed using next-generation sequencing techniques and a total of 20 different mutations were detected. ird11 is resistant to oxidative stress without calorie restriction, whereas ird5 displays an adaptive response against oxidative stress. Candidate 9 mutations, which are thought to be responsible for ird5 and ird11 mutant phenotypes, were investigated via forward and reverse mutations by using various cloning techniques.ConclusionThe results of this study contribute to the basic sciences by showing the relationship between glucose sensing/signaling and oxidative stress response components.

Oyster Mushroom (Pleurotus Ostreatus) Ethanolic Extract Protects Yeast Cells From Hyper- And Hypo-Glycemic Stress

“…Glucose is not able to freely diffuse through the cytoplasmic membrane, thou it is taken up by the cells and metabolized to drive various energy-consuming cellular processes required for cell viability. In addition, glucose regulates the expression of genes predominantly those that encode proteins of the glycolytic and respiratory pathways, stress response, utilization of glucose or alternative carbon sources, or those that influence cell growth (Yilmazer et al, 2021). Manipulating the glucose concentration in the diet or growth media has multiple implications (Tarhan & Çakır, 2021).…”