BackgroundGlucose both is the favorite carbon and energy source and acts as a hormone that plays a regulated role in many biological processes. Calorie restriction extended lifespan in many organisms, including Schizosaccharomyces pombe, while uptake of high glucose led to undesired results, such as diabetes and aging.Methods and ResultsIn this study, sequence analysis of Schizosaccharomyces pombe ird5 and ird11 mutants was performed using next-generation sequencing techniques and a total of 20 different mutations were detected. ird11 is resistant to oxidative stress without calorie restriction, whereas ird5 displays an adaptive response against oxidative stress. Candidate 9 mutations, which are thought to be responsible for ird5 and ird11 mutant phenotypes, were investigated via forward and reverse mutations by using various cloning techniques.ConclusionThe results of this study contribute to the basic sciences by showing the relationship between glucose sensing/signaling and oxidative stress response components.

Glucose is both the favourite carbon and energy source and acts as a hormone that plays a regulating role in many biological processes. Calorie restriction extends the lifespan in many organisms, including Schizosaccharomyces pombe, while uptake of high glucose leads to undesired results, such as diabetes and aging. In this study, sequence analysis of Schizosaccharomyces pombe ird5 and ird11 mutants was performed using next-generation sequencing techniques and a total of 20 different mutations were detected. ird11 is resistant to oxidative stress without calorie restriction, whereas ird5 displays an adaptive response against oxidative stress. We selected nine candidate mutations located in the non-coding (6) and coding (3) region among a total of 20 different mutations. The nine candidate mutations, which are thought to be responsible for ird5 and ird11 mutant phenotypes, were investigated via forward and backward mutations by using various cloning techniques. The results of this study provide report-like information that will contribute to understanding the relationship between glucose sensing/signalling and oxidative stress response components.