Identification of secreted and membrane-bound bat immunoglobulin using a Microchiropteran-specific mouse monoclonal antibody

Lee, William T.; Jones, Derek D.; Yates, Jennifer L.; Winslow, Gary M.; Davis, April; Rudd, Robert J.; Barron, Christopher T.; Cowan, Cailyn

doi:10.1016/j.dci.2016.06.024

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…Only a single variable region light-chain sequence was significantly elevated in the infected bats, which had most similarity to the IgLV7 variable gene family. Studies of big brown bats ( Eptisicus fuscus ) suggest they express predominantly, if not exclusively, λ light chains; thus our findings are similar ( 52 ). Considering that a single light chain was elevated in infected bats, it may be possible to clone this cDNA and coexpress it with each of the 5 heavy-chain sequences described herein to determine if the antibodies are reactive to TCRV antigens.…”

Section: Discussionsupporting

confidence: 79%

Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats

Gerrard

Hawkinson

Sherman

et al. 2017

mSphere

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As reservoir hosts of viruses associated with human disease, little is known about the interactions between bats and viruses. Using Jamaican fruit bats infected with Tacaribe virus (TCRV) as a model, we characterized the gene expression responses to infection in different tissues and identified pathways involved with the response to infection. This report is the most detailed gene discovery work in the species to date and the first to describe immune gene expression responses in bats during a pathogenic viral infection.

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Section: Discussionsupporting

confidence: 79%

Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats

Gerrard

Hawkinson

Sherman

et al. 2017

mSphere

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“…The number of immunoglobulin heavy-chain genes that encode IgG subclasses vary between species, with Seba's fruit bat (Carollia perspicillata) having only one, big brown bat (Eptesicus fuscus) having two, greater short-nosed bat (Cynopterus sphinx) having three and little brown bat having five (48). One monoclonal antibody to the immunoglobulin λ chain of the big brown bat has been generated and it has cross reactivity to little brown bat λ chains; thus, it likely will be useful for characterizing antibodies from these bats (49).…”

Section: Immunoglobulin Repertoires Of Batsmentioning

confidence: 99%

Immunological Control of Viral Infections in Bats and the Emergence of Viruses Highly Pathogenic to Humans

et al. 2017

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Bats are reservoir hosts of many important viruses that cause substantial disease in humans, including coronaviruses, filoviruses, lyssaviruses, and henipaviruses. Other than the lyssaviruses, they do not appear to cause disease in the reservoir bats, thus an explanation for the dichotomous outcomes of infections of humans and bat reservoirs remains to be determined. Bats appear to have a few unusual features that may account for these differences, including evidence of constitutive interferon (IFN) activation and greater combinatorial diversity in immunoglobulin genes that do not undergo substantial affinity maturation. We propose these features may, in part, account for why bats can host these viruses without disease and how they may contribute to the highly pathogenic nature of bat-borne viruses after spillover into humans. Because of the constitutive IFN activity, bat-borne viruses may be shed at low levels from bat cells. With large naive antibody repertoires, bats may control the limited virus replication without the need for rapid affinity maturation, and this may explain why bats typically have low antibody titers to viruses. However, because bat viruses have evolved in high IFN environments, they have enhanced countermeasures against the IFN response. Thus, upon infection of human cells, where the IFN response is not constitutive, the viruses overwhelm the IFN response, leading to abundant virus replication and pathology.

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“…This antibody was generated using www.nature.com/scientificreports www.nature.com/scientificreports/ sera from ERBs, as well as sera from nine other bat species comprising four chiropteran families (manufacturer product datasheet). While this antibody has been confirmed to react with sera from these 10 chiropteran species and has been used with success to detect IgG antibodies in sera from >27 bat species comprising seven chiropteran families [48][49][50][51][52][53][54][55][56][57] , it is possible that its reactivity with IgG antibodies from divergent bat species might diminish. If this is the case, the concentration of secondary antibody may need to be optimized prior to testing a new species of bat or an alternative secondary antibody may need to be used.…”

Section: Filovirus Antigen Lysate Cutoffmentioning

confidence: 99%

Comparative analysis of serologic cross-reactivity using convalescent sera from filovirus-experimentally infected fruit bats

Schuh

Amman

Sealy

et al. 2019

Sci Rep

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With the exception of Reston and Bombali viruses, the marburgviruses and ebolaviruses (family Filoviridae ) cause outbreaks of viral hemorrhagic fever in sub-Saharan Africa. The Egyptian rousette bat (ERB) is a natural reservoir host for the marburgviruses and evidence suggests that bats are also natural reservoirs for the ebolaviruses. Although the search for the natural reservoirs of the ebolaviruses has largely involved serosurveillance of the bat population, there are no validated serological assays to screen bat sera for ebolavirus-specific IgG antibodies. Here, we generate filovirus-specific antisera by prime-boost immunization of groups of captive ERBs with all seven known culturable filoviruses. After validating a system of filovirus-specific indirect ELISAs utilizing infectious-based virus antigens for detection of virus-specific IgG antibodies from bat sera, we assess the level of serological cross-reactivity between the virus-specific antisera and heterologous filovirus antigens. This data is then used to generate a filovirus antibody fingerprint that can predict which of the filovirus species in the system is most antigenically similar to the species responsible for past infection. Our filovirus IgG indirect ELISA system will be a critical tool for identifying bat species with high ebolavirus seroprevalence rates to target for longitudinal studies aimed at establishing natural reservoir host-ebolavirus relationships.

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Identification of secreted and membrane-bound bat immunoglobulin using a Microchiropteran-specific mouse monoclonal antibody

Cited by 7 publications

References 34 publications

Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats

Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats

Immunological Control of Viral Infections in Bats and the Emergence of Viruses Highly Pathogenic to Humans

Comparative analysis of serologic cross-reactivity using convalescent sera from filovirus-experimentally infected fruit bats

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