Identification of sequences expressed during compatible black pepper—Fusarium solani f. sp. piperis interaction

Abstract

Black pepper (Piper nigrum L.) is one of the most widely used spices in the world. Root rot disease is induced by Fusarium solani f. sp. piperis and causes severe yield losses of this crop in the Amazon region. In this work we used the suppression subtractive hybridization to identify differentially expressed sequences in roots of black pepper infected by F. solani f. sp. piperis. Sequences coding for putative proteins related to oxidative burst and defense response, such as superoxide dismutase, cytochrome p450, and alpha-amylase inhibitors/lipid transfer protein, comprised 28.4% of SSH clones according to computational analyses. Furthermore, semi-quantitative RT-PCR assays showed accumulation of putative cysteine proteinase inhibitor and pathogenesis-related protein 4 transcripts at late stage of infection that can help to explain the success of this pathogen in causing root rot disease in black pepper. The results obtained here contribute to improve our understanding about this plant-pathogen interaction.

Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak

“…Most of the studies reported that Fusarium wilt disease is caused by Fusarium. solani (de Souza et al, 2011;Shahnazi et al, 2012). However, some studies claimed that Fusarium.…”

Antifungal Activity and Computational Study of Constituents from Piper divaricatum Essential Oil against Fusarium Infection in Black Pepper

“…Many advances have been made in the knowledge of pathogenic plant fungi, at the cellular and molecular level. The β-glucosidase proteins from fungi are attractive targets for the design of new antifungal agents to control the Fusarium disease in plants [12,13]. Thus, the aim of this work was to provide information on possible roles of the main compounds of the P. divaricatum oil in the mechanisms of action against F. solani f. sp.…”

A comprehensive review on genomic resources in medicinally and industrially important major spices for future breeding programs: Status, utility and challenges

“…For black pepper, 87 ESTs, were reveled through Suppression subtractive hybridization (SSH) sequences from roots infected with F. solani f. sp. Piperis, uncovering relationships among host pathogen nexuses ( de Souza et al, 2011 ). Trait- and tissue-specific (such as clove, scion, root, stem, leaf, flower, rhizome, mixed bulb, seed, tuber, fruit, salt, etc.).…”

“…Among these, saffron, an economically important spice, harbors 6767 publicly available ESTs, of which approximately 6224 are from scar tissue and 151 from tuber tissue ( D'Agostino et al, 2007 ). Spice improvement programmes can optimize breeding strategies to, accelerate the development of new and improved spice varieties, by understanding gene expression patterns, identifying genes for specific traits, alternative splicing and transcript diversity, marker development and genomic mapping, and functional genomics by leveraging discussed EST resources ( D'Agostino et al, 2007 ; de Souza et al, 2011 ; Chand et al, 2015 ).…”