Identification of several proteins involved in regulation of sperm motility by proteomic analysis

Zhao, Chun; Huo, Ran; Wang, Fuqiang; Lin, Min; Zhou, Zuomin; Sha, Jiahao

doi:10.1016/j.fertnstert.2006.06.057

Cited by 147 publications

(116 citation statements)

References 15 publications

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“…We identified isocitrate dehydrogenase subunit a as two protein spots that exhibited lower expression in asthenozoospermic patients, whereas three other enzymes (phosphoglycerate mutase 2, triosephosphate isomerase and glutamate oxaloacetate transaminase-1) were highly expressed in most of the patients. 49 Among these findings, those relating to triosephosphate isomerase were recently validated by Siva et al. 50 Their study used the same strategy to analyse differentially expressed proteins in asthenozoospermic patients, and eight proteins with higher triosephosphate isomerase were identified in patients.…”

Section: Functional Proteomics Of a Human Model Of Abnormal Spermatogmentioning

confidence: 89%

“…Second, with western blotting, antibodies specific for PTMs can identify spots in a gel containing proteins with these modifications. 60 In our studies, 21,22,32,37,39,49,57 most of the proteome profiles related to spermatogenesis were established by 2DE-MS, and many proteins were represented by two or more spots in the gel, which indicated PTMs such as phosphorylation.…”

Section: Hormone-induced Suppression Model Of Spermatogenesismentioning

confidence: 89%

“…49 Two of these spots represented 14 kDa fragments and the other two were 17 kDa fragments. All of these spots were highly expressed in asthenozoospermic patients.…”

Section: Functional Proteomics Of a Human Model Of Abnormal Spermatogmentioning

confidence: 97%

“…In the other studies, 50,51 asthenozoospermic samples were defined as those with 25% progressive motility (grade A) or 50% motile sperm (grades A1B). The samples in our study 49 were from patients with severe asthenozoospermia, and we think that the proteins identified represent clinically relevant molecular targets for sperm motility that will provide a better understanding of male infertility.…”

Section: Functional Proteomics Of a Human Model Of Abnormal Spermatogmentioning

confidence: 99%

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Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Huang¹,

Sha²

2010

Asian J Androl

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Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function. The present article reviews the available scientific literature related to spermatogenesis. In addition, this study uses two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC)-MS to construct a series of proteome profiles describing spermatogenesis. This large-scale identification of proteins provides a rich resource for elucidating the mechanisms underlying male fertility and infertility.

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Section: Functional Proteomics Of a Human Model Of Abnormal Spermatogmentioning

confidence: 89%

Section: Hormone-induced Suppression Model Of Spermatogenesismentioning

confidence: 89%

“…49 Two of these spots represented 14 kDa fragments and the other two were 17 kDa fragments. All of these spots were highly expressed in asthenozoospermic patients.…”

Section: Functional Proteomics Of a Human Model Of Abnormal Spermatogmentioning

confidence: 97%

Section: Functional Proteomics Of a Human Model Of Abnormal Spermatogmentioning

confidence: 99%

See 2 more Smart Citations

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Huang¹,

Sha²

2010

Asian J Androl

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“…To date, 34 proteins responsible for sperm motility have been detected in asthenozoospermic patients, as differentially expressed proteins compared with normozosprmic ones, including Tektin 1, glycerol kinase testis specific, isocitrate dehydrogenase subunit α, heat shock 70 kDa related protein 2, cytochrome c oxidase subunit 6B, prolactin-induced protein, outer dense fiber protein 2, semenogelin, and others [9,15,16], and the number of proteins involved in sperm motility identified by proteomic methods is increasing [9,15].…”

Section: Introductionmentioning

confidence: 99%

A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia

Hashemitabar

Sabbagh

Orazizadeh

et al. 2015

J Assist Reprod Genet

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Purpose Asthenozoospermia is a common cause of human male infertility characterized by reduced sperm motility. The molecular mechanism that impairs sperm motility is not fully understood. This study proposed to identify novel biomarkers by focusing on sperm tail proteomic analysis of asthenozoospermic patients. Methods Sperm were isolated from normozoospermic and asthenozoospermic semen samples. Tail fractions were obtained by sonication followed by Percoll gradient. The proteins were extracted by solubilization and subjected to two-dimensional gel electrophoresis (2-DE); then, the spots were analyzed using Image Master 2D Platinum software. The significantly increased/ decreased amounts of proteins in the two groups were exploited by matrix-assisted laser desorption-ionization time-of-flight/ time-of-flight (MALDI-TOF-TOF) mass spectrometry. Results Three hundred ninety protein spots were detected in both groups. Twenty-one protein spots that had significantly altered amounts (p<0.05) were excised and exploited using MALDI-TOF-TOF mass spectrometry. They led to the identification of the following 14 unique proteins: Tubulin beta 2B; glutathione S-transferase Mu 3; keratin, type II cytoskeletal 1; outer dense fiber protein 2; voltage-dependent anionselective channel protein 2; A-kinase anchor protein 4; cytochrome c oxidase subunit 6B; sperm protein associated with the nucleus on the X chromosome B; phospholipid hydroperoxide glutathione peroxidase-mitochondrial; isoaspartyl peptidase/L-asparaginase; heat shock-related 70 kDa protein 2; stress-70 protein, mitochondrial; glyceraldehyde-3-phosphate dehydrogenase, testis-specific and clusterin. Conclusion Fourteen proteins present in different amounts in asthenozoospermic sperm tail samples were identified, four of which are reported here for the first time. These proteins might be used as markers for the better diagnosis of sperm dysfunctions, targets for male contraceptive development, and to predict embryo quality.

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Detection of porcine sperm microRNAs using a heterologous microRNA microarray and reverse transcriptase polymerase chain reaction

Curry

Ellis

Pratt

2008

Molecular Reproduction Devel

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Identification of several proteins involved in regulation of sperm motility by proteomic analysis

Cited by 147 publications

References 15 publications

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia

Detection of porcine sperm microRNAs using a heterologous microRNA microarray and reverse transcriptase polymerase chain reaction

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