Identification of Signaling Pathways Mediating Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis in Human Trophoblasts

Inaba, Hiroaki; Kuboniwa, Masae; Sugita, Hideyuki; Lamont, Richard J.; Amano, Atsuo

doi:10.1128/iai.00258-12

Cited by 38 publications

(35 citation statements)

References 63 publications

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“…It is well accepted that factors from fetal or placental origin as well as maternal factors are likely to have a potential role in the regulation of inflammation, lesion, and apoptosis . Existing evidence supports the concept that P. gingivalis induces inflammation and leads to apoptosis in trophoblasts . Therefore, we put forward an assumption that P. gingivalis is a threat to the outcomes of pregnancy through influencing signaling molecules or mediators of the local fetoplacental unit.…”

Section: Discussionmentioning

confidence: 81%

“…Porphyromonas gingivalis ATCC 33277 (American Type Culture Collection, Manassas, VA) was used in our investigation and was maintained on Columbia blood plates under anaerobic conditions with 10% H 2 , 10% CO 2 and 80% N 2 at 37°C. Bacterial cells were also cultivated in a trypticase soy broth supplied with 1 mg/mL yeast extract, 5 mg/mL hemin, and 1 mg/mL menadione under the same conditions as previously described . For the periodontal inoculum, broth cultures were grown to achieve their log phase of growth, and the bacterial cells were then centrifuged and washed with phosphate‐buffered saline (PBS) solution three times and resuspended in PBS.…”

Section: Methodsmentioning

confidence: 99%

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Periodontal infection with Porphyromonas gingivalis induces preterm birth and lower birth weight in rats

Liang

Ren²,

Guo

et al. 2018

Molecular Oral Microbiology

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Preterm birth (PTB), accompanied by low birth weight (LBW) or not, is a syndrome with tremendous risk factors and long-term health consequences for children. In recent decades, overwhelming studies have shown that periodontitis contributes to prematurity and LBW. This study was conducted to determine the link between maternal periodontitis and the pathogenesis of PTB and/or LBW through a rat infection model induced by Porphyromonas gingivalis, an important periodontopathic bacterium. The murine model was established by surgically ligating the left mandibular first molars and inoculating with P. gingivalis, and then all female rats initiated mating 6 weeks post infection. The gestational day and birth weight were recorded, and blood, amniotic fluid, and placental specimens were collected. Rats with a PTB and LBW newborns were observed in the P. gingivalis-infected group. Additionally, P. gingivalis infection significantly increased the maternal serum levels of interferon-γ and interleukin-1β, whereas no significant difference in the cytokine response was observed in the amniotic fluid. Moreover, with the translocation of P. gingivalis to placentas, remarkable changes in gestational tissues were found, followed by significantly enhanced expression of Toll-like receptor 2 (TLR2) as well as Fas and Fas ligand (FasL). These results support the concept that severe cases of periodontitis caused by P. gingivalis infection may be indicative of rats being more susceptible to PTB/LBW, probably through the activation of the TLR2 and Fas/FasL pathways within the placental tissues. This study gave us new insight into how maternal periodontopathogens might be linked to placental damage and premature pathogenesis.

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Section: Discussionmentioning

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Periodontal infection with Porphyromonas gingivalis induces preterm birth and lower birth weight in rats

Liang

Ren²,

Guo

et al. 2018

Molecular Oral Microbiology

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“…Adult humans and chickens show a similar pattern where high expression of Ets1 is found mainly in lymphoid tissues [55–57]. Ets1 is expressed in B cells, T cells, NK cells, and NK T cells [21, 50, 56–62] and is inducible in other, non-lymphoid cell types in response to certain stimuli (for example, [63–65]).…”

Section: Expression Patterns Of Ets1mentioning

confidence: 99%

Review of Ets1 structure, function, and roles in immunity

Garrett‐Sinha

2013

Cell. Mol. Life Sci.

170

124

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The Ets1 transcription factor is a member of the Ets gene family and is highly conserved throughout evolution. Ets1 is known to regulate a number of important biological processes in normal cells and in tumors. In particular, Ets1 has been associated with regulation of immune cell function and with an aggressive behavior in tumors that express it at high levels. Here we review and summarize the general features of Ets1 and describe its roles in immunity and autoimmunity, with a focus on its roles in B lymphocytes. We also review evidence that suggests that Ets1 may play a role in malignant transformation of hematopoietic malignancies including B cell malignancies.

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“…P. gingivalis can invade HTR8/SvNeo cells and inhibit their proliferation through induction of G1 arrest and apoptosis [93,94]. Further characterization of this phenotype shows that invasion of HTR8/SvNeo by P. gingivalis induces IFN-γ expression [94] and activates the DNA damage response in these cells that is facilitated by gingipain degradation of the p53 antagonist, MDM2 [95]. While EVT death could explain decreased density of these cells in the placental bed, paracrinemediated inhibition of EVT invasion may also be a factor.…”

Section: P Gingivalis As a Vascular Pathogen Of The Placental Bedmentioning

confidence: 99%

Porphyromonas gingivalisand adverse pregnancy outcome

Reyes

Phillips

Wolfe

et al. 2017

Journal of Oral Microbiology

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Porphyromonas gingivalis is a Gram-negative, anaerobic bacterium considered to be an important pathogen of periodontal disease that is also implicated in adverse pregnancy outcome (APO). Until recently, our understanding of the role of P. gingivalis in APO has been limited and sometimes contradictory. The purpose of this review is to provide an overview of past and current research on P. gingivalis that addresses some of the controversies concerning the role of this organism in the pathogenesis of APO.ARTICLE HISTORY

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Identification of Signaling Pathways Mediating Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis in Human Trophoblasts

Cited by 38 publications

References 63 publications

Periodontal infection with Porphyromonas gingivalis induces preterm birth and lower birth weight in rats

Periodontal infection with Porphyromonas gingivalis induces preterm birth and lower birth weight in rats

Review of Ets1 structure, function, and roles in immunity

Porphyromonas gingivalisand adverse pregnancy outcome

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