Identification of Silver Nanoparticles in the Small Intestinal Mucosa, Liver, and Spleen of Rats by Transmission Electron Microscopy

Platonova, T. A.; Придворова, С. М.; Zherdev, Anatoly V.; Vasilevskaya, L. S.; Arianova, E A; Gmoshinski, I. V.; Хотимченко, С А; Dzantiev, Boris B.; Popov, Vladimir O.; Tutelyan, V A

doi:10.1007/s10517-013-2122-4

Cited by 15 publications

(8 citation statements)

References 6 publications

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“…Interestingly, even with the significant accumulation in the tissue, minimal damage was observed. The lack of damage could be attributed to the considerable amount of coating agent, enhanced by its high clearance rate, as we have shown in previous works [18,21,[64][65][66][67]. These features definitely contribute to the wide therapeutic window found for the five formulations assessed in this work.…”

Section: Discussionsupporting

confidence: 68%

Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio

et al. 2021

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Silver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor cells (HCT-15), their cytotoxic effect on primary mouse cultures, and their lethal dose on BALB/c mice. The evaluated AgNP formulations have a composition within the ranges Ag: 1.14–1.32% w/w, PVP: 19.6–24.5% and H2O: 74.2–79.2% with predominant spherical shape within an average size range of 16–30 nm according to transmission electron microscopy (TEM). All formulations assessed increase mitochondrial ROS concentration and induce apoptosis as the leading death pathway on HCT-15 cells. Except for AgNP1, the growth inhibition potency of AgNP formulations of human colon tumor cancer cells (HCT-15) is 34.5 times higher than carboplatin, one of the first-line chemotherapy agents. Nevertheless, 5–10% of necrotic events, even at the lower concentration evaluated, were observed. The cytotoxic selectivity was confirmed by evaluating the cytotoxic effect on aorta, spleen, heart, liver, and kidney primary cultures from BALB/c mice. Despite the cytotoxic effects observed in vitro, the lethal dose and histopathological analysis showed the low toxicity of these formulations (all of them on Category 4 of the Globally Harmonized System of Classification and Labelling of Chemicals) and minor damage observed on analyzed organs. The results provide an additional example of the rational design of safety nanomaterials with antitumor potency and urge further experiments to complete the preclinical studies for these AgNP formulations.

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Section: Discussionsupporting

confidence: 68%

Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio

et al. 2021

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“…Platonova et al (2013) noted accumulation of AgNP in the liver and spleen of rats after injection of AgNP solution. In adult zebrafish exposed to AgNP, accumulation was also noted in the liver (Choi et al 2010).…”

Section: Discussionmentioning

confidence: 96%

Analysis of hematologic alterations, immune responses and metallothionein gene expression in Nile tilapia (Oreochromis niloticus) exposed to silver nanoparticles

Thummabancha

Onparn

Srisapoome

2016

Journal of Immunotoxicology

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“…The effective concentrations employed attributed to the high stability provided by the high amount of PVP used to obtain this AgNP formulation. Besides, biodistribution studies on rats show that 98% of this formulation was excreted in the feces [ 24 , 25 , 26 ], a result that confirms its low toxicity. To better understand the toxicological profile, an evaluation of the hemolytic effect of this formulation on human erythrocytes is indispensable.…”

Section: Introductionmentioning

confidence: 93%

Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study

et al. 2021

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The use of nanomaterials is becoming increasingly widespread, leading to substantial research focused on nanomedicine. Nevertheless, the lack of complete toxicity profiles limits nanomaterials’ uses, despite their remarkable diagnostic and therapeutic results on in vitro and in vivo models. Silver nanoparticles (AgNPs), particularly Argovit™, have shown microbicidal, virucidal, and antitumoral effects. Among the first-line toxicity tests is the hemolysis assay. Here, the hemolytic effect of Argovit™ AgNPs on erythrocytes from one healthy donor (HDE) and one diabetic donor (DDE) is evaluated by the hemolysis assay against AgNO3. The results showed that Argovit™, in concentrations ≤24 µg/mL of metallic silver, did not show a hemolytic effect on the HDE or DDE. On the contrary, AgNO3 at the same concentration of silver ions produces more than 10% hemolysis in both the erythrocyte types. In all the experimental conditions assessed, the DDE was shown to be more prone to hemolysis than the HDE elicited by Ag+ ions or AgNPs, but much more evident with Ag+ ions. The results show that Argovit™ is the least hemolytic compared with the other twenty-two AgNP formulations previously reported, probably due to the polymer mass used to stabilize the Argovit™ formulation. The results obtained provide relevant information that contributes to obtaining a comprehensive toxicological profile to design safe and effective AgNP formulations.

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Identification of Silver Nanoparticles in the Small Intestinal Mucosa, Liver, and Spleen of Rats by Transmission Electron Microscopy

Cited by 15 publications

References 6 publications

Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio

Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio

Analysis of hematologic alterations, immune responses and metallothionein gene expression in Nile tilapia (Oreochromis niloticus) exposed to silver nanoparticles

Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study

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