Identification of Single Nucleotide Polymorphism on Growth Hormone Gene in Aceh Cattle

Sari, Eka Puspita; Noor, Ronny Rachman; Sumantri, Cece; Yunus, Md.; L, Han J; Muladno,

doi:10.5398/medpet.2013.36.1.21

Cited by 9 publications

(7 citation statements)

References 6 publications

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“…The first mutation is 2230 C>T SNP which appeared in the Nyalawi subtype only. A similar result was mentioned by another group in Indonesia [18] who reported the same C>T SNP as a new mutation in The second mutation (2258 bp C>T) appeared in the two strains of Baggara cattle. The 2258 C>T SNP was previously associated with average daily weight gain (ADG) and carcass weight (CWT) [19] .…”

Section: Discussionsupporting

confidence: 84%

Yerli Sudan Baggara Sığırında Büyüme Hormonu ve İnsülin Benzeri Büyüme Faktörü 1 Genlerinin Seçili Genetik Polimorfizmlerinin Moleküler Tespiti

Omer¹,

Masri²,

Jawasreh³

et al. 2018

Kafkas Univ Vet Fak Derg

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The study involved 127 bulls of two geographically separate strains of Sudanese Baggara zebu cattle (Nyalawi and Mesairi). The target was to investigate two polymorphisms in growth hormone gene (M57764) and insulin like growth factor1 gene (000162.1). PCR-RFLP was used to genotype DNA samples and DNA sequencing was used to check the accuracy of genotyping results using selected samples. Available sequences were analyzed using BioEdit and MEGA6 softwares. Some population genetic measures in the two strains of Baggara zebu cattle were investigated. PCR-RFLP revealed that the two Baggara cattle strains have high genetic similarity at position 2141C>G of the GH1 gene (monomorphic showing the ancestral allele C/C). There were no differences between the two strains at position-472C>T of the IGF-1 gene promoter. The mutant homozygote (TT) was detected in the Mesairi strain only with a frequency of 0.016. The heterozygote (CT) genotype existed in the two strains with low allele frequencies (0. 068 and 0.079 for Nyalawi and Mesairi respectively). Moreover, three mutations were identified in exon5 of the GH1 sequence including two silent mutations at positions 2230 (C>T) and 2291(A>C) and a third transition mutation at position 2258 (C/T) detected in the sequences of the two strains. In conclusion, the two strains were found to be genetically similar at target positions. The detected mutation at exon 5 of GH1 (2258 (C/T) should be validated.

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Section: Discussionsupporting

confidence: 84%

Yerli Sudan Baggara Sığırında Büyüme Hormonu ve İnsülin Benzeri Büyüme Faktörü 1 Genlerinin Seçili Genetik Polimorfizmlerinin Moleküler Tespiti

Omer¹,

Masri²,

Jawasreh³

et al. 2018

Kafkas Univ Vet Fak Derg

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“…The variation of Single Nucleotide Polymorphism (SNP) which was identified in the third intron region from GH gene on several cattle are shown in Table 2. Sari et al (2011) reported one new mutation on fifth exon on 2230 bp, in which C nucleotide turned into T nucleotide, and this was called silent mutation (CTC/Leu > CTT/Leu). Based on the sequencing result, it was concluded that the sequence of Aceh cattle on third intron was similar to Bos indicus group (Vechur, Butana, Kenana).…”

Section: Sequencing For Gh Mspi Genementioning

confidence: 99%

GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

Putra

Hartatik

Sumadi

2013

J. Indonesian Trop. Anim. Agric.

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ABSTRAKPenelitian ini bertujuan untuk mengidentifikasi genotip gen growth hormone (GH) pada sapi Aceh yang diseleksi di Balai Pembibitan Ternak Unggul -Hijauan Pakan Ternak (BPTU-HPT) Sapi Aceh Indrapuri. Empat puluh satu ekor sapi Aceh terdiri dari 21 jantan dan 20 betina digunakan dalam penelitian ini. Ekstraksi DNA genom menggunakan darah sesuai metode Sambrook et al. (1989). Analisis Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) dan sekuensing digunakan untuk melihat situs restriksi MspI pada gen GH. Berdasarkan hasil sekuensing diketahui bahwa semua sampel sapi Aceh bersifat monomorfisme. Frekuensi genotip TT dan alel T masing-masing sebesar 1,00. Mutasi transisi C (cytosine) menjadi T (thymine) pada posisi 1548 bp menyebabkan situs restriksi MspI tidak ditemukan. Kata kunci: Sapi Aceh, gen GH, PCR-RFLP, sekuensing, enzim restriksi MspI ABSTRACTThe objective of this research was to identify growth hormone (GH) genes genotype in selected Aceh cattle at Indrapuri's Breeding and Forage Centre (IBFC) of Aceh Cattle. Fourty one cattle consisting of 21 male and 20 female cattle were used in this study. The genomic DNA was extracted from blood using Sambrook et al. (1989) methods. Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) and mehod of sequencing was used to detect MspI site on GH gene. Based on sequencing results, it can be concluded that all cattle were monomorphism. The frequency of TT genotype were 1.00 and same as T allele frequency. The transition of C (cytosine) into T (thymine) on 1548 bp caused the lost of restriction site.

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“…The result shows the introduction of valine allele in crossbred beef cattle, such as two genotypes LL and LV were observed in Ongole grade cattle (96.15%LL, 3.85%LV), Limousin-Madura (81.46%LL, 18.52%LV), Limousin-Ongole grade cattle (78.57%LL, 21.43%LV), and Limousin (66.67%LL, 33.33%LV). Single nucleotide polymorphism of growth hormone gene in beef cattle in several countries also has been published by several researchers (Akcay et al 2015;Akis Akad et al 2012;Dario et al 2005;Deepika and Salar 2013;Korkmazagaoglu and Akyuz 2013;Moravčíková et al 2012;Reis et al 2001;Sari et al 2013, Sutarno 2010.…”

Section: Introductionmentioning

confidence: 81%

Short communication: The genotype of growth hormone gene that affects the birth weight and average daily gain in crossbred beef cattle

et al. 2020

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Abstract. Hartatik T., Fathoni A, Bintara S., Ismaya, Panjono, Widyobroto B.P, Agus A, Budisatria I.G.S, Leroy P. 2020. Short communication: The genotype of growth hormone gene that affects the birth weight and average daily gain in crossbred beef cattle. Biodiversitas 21: 941-945. Growth Hormone gene has been proposed to direct search for quantitative trait loci, and polymorphisms at these loci have been associated with several production traits in bovine. This research aimed to investigate the association of the genotype of partial growth hormone gene with birth weight and average daily gain in crossbred beef cattle. The materials of this research consist of 47 samples from four different breeds. Birth weight was measured just after calving delivery from the mother. The average daily gain was calculated from the period since birth weight to the second weight (90 days). Genotyping of the growth hormone gene was determined by the sequencing and PCR-RFLP method with the AluI restriction enzyme. The association between genotype and growth traits data was analyzed using analysis of variance (ANOVA) by RStudio version 1.1.383. The results showed the significantly different (P<0.001) in birth weight and average daily gain according to the different breed. Friesian Holstein breed showed the highest birth weight, followed by Belgian Blue Bull cross and others. The genotype valine/valine only occurs in Belgian blue bull cross and Wagyu bull cross cattle and showed moderate birth weight. Genotype affects birth weight significantly (P<0.001). The birth weight for genotype leucine/leucine, leucine/valine, and valine/valine was 34.82±18.13, 25.31±13.10, and 29.35±13.65, respectively. There was no significant difference in average daily genes according to the different genotypes. In conclusion, the growth hormone gene was probably one of the molecular genetics markers for excellent growth traits in different crossbred beef cattle.

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Identification of Single Nucleotide Polymorphism on Growth Hormone Gene in Aceh Cattle

Cited by 9 publications

References 6 publications

Yerli Sudan Baggara Sığırında Büyüme Hormonu ve İnsülin Benzeri Büyüme Faktörü 1 Genlerinin Seçili Genetik Polimorfizmlerinin Moleküler Tespiti

Yerli Sudan Baggara Sığırında Büyüme Hormonu ve İnsülin Benzeri Büyüme Faktörü 1 Genlerinin Seçili Genetik Polimorfizmlerinin Moleküler Tespiti

GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

Short communication: The genotype of growth hormone gene that affects the birth weight and average daily gain in crossbred beef cattle

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