Identification of six transcripts encoding putative receptor-like protein kinase (RLK) and their expression profiles in Vitis flexuosa infected with pathogens

Islam, Md. Zaherul; Ahn, Soon Young; Yun, Hae Keun

doi:10.1016/j.scienta.2015.05.025

Cited by 8 publications

(6 citation statements)

References 41 publications

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“…NBS-LRRs function in defense responses, while RLKs have been implicated in a wide range of signaling pathways as transmembrane proteins with putative extracellular domains and intracellular protein kinase domains [42]. Recently, a study of grapevine defense responses to E. ampelina identified six genes encoding RLKs that were highly induced [44].We found both NBS-LRRs and RLKs to be abundant in both HR and HS grapes, consistent with a role in resistance to E. ampelina infection.…”

Section: Discussionsupporting

confidence: 75%

Comparative transcriptome analysis of leaves from two grapevine species reveals an early gene expression profile associated with Elsinoe ampelina resistance

Min

Wan

Zhu

et al. 2020

Preprint

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Background: Anthracnose ( Elsinoe ampelina ) causes extensive damage to grapevine ( Vitis vinifera ) production worldwide, but the defense mechanisms exhibited by grape are not well understood. Results: In present study, the transcriptome differences of two grape species that exhibit either strong resistance (HR) or sensitivity (HS) to E . ampelina were determined at different time points up to 72 hours post infection (hpi) using RNA-seq profiling. Approximately 172 million high quality reads were obtained from a total of 40 samples. As a result, 3414 differentially expressed genes (DEGs) were identified, with 2,246 in the HR grape V . q uinquangularis Shang-24 accession and 2,019 in the HS grape V . vinifera Red Globe. More up-regulated than down-regulated genes were identified both in the HR and HS samples at each time point except 48 hours post infection. Conclusions: Gene ontology (GO) function and pathway enrichment analysis suggested that the grape transcriptional response to E . ampelina infection involves genes encoding protein kinases, transcription factors, metabolite synthesis, and phytohormone signaling. Although most of the GO functional categories and enriched pathways in response to E . ampelina infection were the same in the two species, the response was apparent much earlier in the HR grape (6 hours post infection and 24 hours post infection) than in the HS grape (48 hours post infection and 72 hours post infection), which may be associated with the contrasting resistance phenotypes. This study provides new insights into the grape defense system involved in responses to E . ampelina infection, and has identified several candidate genes that may be exploited in future biotechnological approaches to increase disease resistance in grapevine.

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Section: Discussionsupporting

confidence: 75%

Comparative transcriptome analysis of leaves from two grapevine species reveals an early gene expression profile associated with Elsinoe ampelina resistance

Min

Wan

Zhu

et al. 2020

Preprint

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“…In our previous studies, 33 resistance and resistance related genes of five groups, RLK, RPS5-like, Mlo-like, CXE, and EDL, from V. flexuosa inoculated with E. ampelina were characterized and found to have different expression levels (Islam et al 2015a;Islam et al 2015b). These findings indicate that all of these genes may be involved in plant resistance responses against different pathogens in V. flexuosa native to Korea.…”

Section: Discussionmentioning

confidence: 89%

“…Among Vitis species native to Korea, the wild cultivar V. flexuosa has been reported to show resistant responses such as expression of defense related genes against pathogen attacks (Ahn et al 2012). We previously characterized 33 resistance and resistance related genes of five groups, receptor-like protein kinase (RLK), resistance to Pseudomonas syringae 5-like (RPS5-like), mildew resistance locus o-like (Mlo-like), carboxylesterse (CXE), and enhanced disease susceptibility1-like (EDL) from transcriptome analysis based on the next generation sequencing of V. flexuosa inoculated with Elsinoë ampelina (Islam et al 2015a;Islam et al 2015b). R and R-related genes showed responses with different expression patterns against different pathogens in many plants ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Expression of Genes Related with Defense Responses against Pathogen Infections in Vitis flexuosa

Islam¹,

Ahn²,

Kim³

et al. 2016

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Quantitative real-time PCR revealed that R and R-related genes in V. flexuosa induced defense responses through expression of genes in SA-pathway rather than in the JA-pathway, and R-related genes were more closely correlated with lignin and phytoalexin biosynthesis than anthocyanin biosynthesis against different pathogen infections in V. flexuosa. Specifically, genes such as VfRLK586, VfRLK2422, VfRLK5099, VfRLK29610, VfRLK55257, VfRPS5-like4135, VfRPS5-like4832, VfRPS5-like20585, VfRPS5-like55532, VfEDL2, VfEDL3, VfCXE5585, VfCXE12827, and VfCXE13132 showed close correlation with induction of genes in the SA-mediated pathway, lignin, phytoalexin, and anthocyanin biosynthesis. Genes including VfRLK2422, VfRLK5099, VfRLK29610, VfRPS5-like55532, VfEDL3, and VfCXE12827 were also correlated with the JA-mediated signaling pathway in induction of defense responses.

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“…It has also been reported that V. vinifera HSR1 transcripts were undetectable at 18 to 48 hpi and were upregulated at later time points after inoculation of Plasmopara viticola ( Chong et al, 2008 ), which is similar to the expression of VfCXE transcripts against R. vitis . Islam et al (2015a , 2015b) also reported that several receptor-like protein kinases and CC-NBS-LRR genes, which are considered potential plant resistance genes, showed up-and down-regulated expression against plant pathogens. Therefore, the change in expression level of all tested VfCXE genes after pathogen infection indicated that VfCXE might play a role in signal transduction pathways for disease resistance or a correlated response, similar to other characterized carboxylesterase genes.…”

Section: Resultsmentioning

confidence: 98%

Identification and Expression Profiles of Six Transcripts Encoding Carboxylesterase Protein in Vitis flexuosa Infected with Pathogens

Islam¹,

Yun²

2016

The Plant Pathology Journal

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Plants protect themselves from pathogen attacks via several mechanisms, including hypersensitive cell death. Recognition of pathogen attack by the plant resistance gene triggers expression of carboxylesterase genes associated with hypersensitive response. We identified six transcripts of carboxylesterase genes, Vitis flexuosa carboxylesterase 5585 (VfCXE5585), VfCXE12827, VfCXE13132, VfCXE17159, VfCXE18231, and VfCXE47674, which showed different expression patterns upon transcriptome analysis of V. flexuosa inoculated with Elsinoe ampelina. The lengths of genes ranged from 1,098 to 1,629 bp, and their encoded proteins consisted of 309 to 335 amino acids. The predicted amino acid sequences showed hydrolase like domains in all six transcripts and contained two conserved motifs, GXSXG of serine hydrolase characteristics and HGGGF related to the carboxylesterase family. The deduced amino acid sequence also contained a potential catalytic triad consisted of serine, aspartic acid and histidine. Of the six transcripts, VfCXE12827 showed upregulated expression against E. ampelina at all time points. Three genes (VfCXE5585, VfCXE12827, and VfCXE13132) showed upregulation, while others (VfCXE17159, VfCXE18231, and VfCXE47674) were down regulated in grapevines infected with Botrytis cinerea. All transcripts showed upregulated expression against Rhizobium vitis at early and later time points except VfCXE12827, and were downregulated for up to 48 hours post inoculation (hpi) after upregulation at 1 hpi in response to R. vitis infection. All tested genes showed high and differential expression in response to pathogens, indicating that they all may play a role in defense pathways during pathogen infection in grapevines.

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Identification of six transcripts encoding putative receptor-like protein kinase (RLK) and their expression profiles in Vitis flexuosa infected with pathogens

Cited by 8 publications

References 41 publications

Comparative transcriptome analysis of leaves from two grapevine species reveals an early gene expression profile associated with Elsinoe ampelina resistance

Comparative transcriptome analysis of leaves from two grapevine species reveals an early gene expression profile associated with Elsinoe ampelina resistance

Expression of Genes Related with Defense Responses against Pathogen Infections in Vitis flexuosa

Identification and Expression Profiles of Six Transcripts Encoding Carboxylesterase Protein in Vitis flexuosa Infected with Pathogens

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