Identification of Sjögren’s syndrome oral fluid biomarker candidates following high-abundance protein depletion

Deutsch, Omer; Krief, Guy; Konttinen, Yrjö T.; Zaks, Batia; Wong, David T.; Aframian, Doron J.; Palmon, Aaron

doi:10.1093/rheumatology/keu405

Cited by 55 publications

(36 citation statements)

References 20 publications

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“…[43][44][45][46][47] Imaging would not provide biological proof of mechanism however, or mechanistic understanding in the context of a failed study.…”

Section: Discussionmentioning

confidence: 99%

Standardisation of labial salivary gland histopathology in clinical trials in primary Sjögren's syndrome

et al. 2016

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Labial salivary gland (LSG) biopsy is used in the classification of primary Sjögren's syndrome (PSS) and in patient stratification in clinical trials. It may also function as a biomarker. The acquisition of tissue and histological interpretation is variable and needs to be standardised for use in clinical trials. A modified European League Against Rheumatism consensus guideline development strategy was used. The steering committee of the ad hoc working group identified key outstanding points of variability in LSG acquisition and analysis. A 2-day workshop was held to develop consensus where possible and identify points where further discussion/data was needed. These points were reviewed by a subgroup of experts on PSS histopathology and then circulated via an online survey to 50 stakeholder experts consisting of rheumatologists, histopathologists and oral medicine specialists, to assess level of agreement (0–10 scale) and comments. Criteria for agreement were a mean score ≥6/10 and 75% of respondents scoring ≥6/10. Thirty-nine (78%) experts responded and 16 points met criteria for agreement. These points are focused on tissue requirements, identification of the characteristic focal lymphocytic sialadenitis, calculation of the focus score, identification of germinal centres, assessment of the area of leucocyte infiltration, reporting standards and use of prestudy samples for clinical trials. We provide standardised consensus guidance for the use of labial salivary gland histopathology in the classification of PSS and in clinical trials and identify areas where further research is required to achieve evidence-based consensus.

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“…[43][44][45][46][47] Imaging would not provide biological proof of mechanism however, or mechanistic understanding in the context of a failed study.…”

Section: Discussionmentioning

confidence: 99%

Standardisation of labial salivary gland histopathology in clinical trials in primary Sjögren's syndrome

et al. 2016

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“…Transgenic mice over-expressing CA I showed aggravated joint inflammation and destruction 6 . CA I has been identified as an oral biomarker in oral fluid of Sjogren's syndrome 7 , and antibodies to CA III and IV have been identified in rheumatoid arthritis 8 . In consideration of the paucity of data on CAs in rheumatic diseases, we have studied the presence of CA IX and XII, two emerging CAs which are pivotal in many human pathologies 4 , in the synovial fluid and synovial cells of JIA patients.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the transmembrane carbonic anhydrase isoforms IX and XII in the inflamed synovium

Margheri

Ceruso

Carta

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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Juvenile idiopathic arthritis (JIA) is the most common form of chronic rheumatic disease affecting children worldwide, with some features similar to adult rheumatoid arthritis (RA). In the present study, we aim at investigating novel markers that will allow in the future for tailored, more personalized treatment strategies. Hence, taking notice of several reports proving the role of local acidosis as a causal link between inflammatory diseases and related pain, and the involvement of several carbonic anhydrases (CA, EC 4.2.1.1) isoforms in articular diseases, we evaluated in JIA patients the expression of these metalloenzymes. We identified that JIA patients show high levels of active CA IX and XII isoforms. Our results represent the first evidence of the identification of these enzymes as potential therapeutic targets and development of novel innovative therapies for arthritis, also considering that the two isoforms are validated antitumor targets.

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“…In another study, the depletion of HAPs such as alpha amylase, albumin, and IgG from oral fluids (OFs) has been reported using alpha amylase affinity device, the ProteoPrep immunoaffinity albumin and IgG depletion kit, respectively . The pooled OF sample (from 18 females patients) and pooled healthy controls (from 18 healthy females) were first subjected to the amylase removing device.…”

Section: Methodologies Used To Reduce the Complexity Of Proteomic Sammentioning

confidence: 99%

Liquid‐phase based separation systems for depletion, prefractionation, and enrichment of proteins in biological fluids and matrices for in‐depth proteomics analysis—An update covering the period 2014–2016

Rassi

Puangpila

2016

Electrophoresis

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This review article is an update of our previous one by C. Puangpila, E. Mayadunne, and Z. El Rassi, Electrophoresis 2015, 36, 238-252. Similarly to the previous article, this review has two main topics, including (i) proteomic sample preparation (e.g., depletion of high-abundance proteins, reduction of the protein dynamic concentration range, and enrichment of a particular subproteome), and (ii) the subsequent chromatographic and/or electrophoretic prefractionation prior to protein separation and identification by LC-MS/MS. More than 70 papers published in the period extending from mid-2014 to the present have been reviewed. Although an effort was made to yield a comprehensive review article, one may safely state that this review article is by no means thorough, and the aim was to rather provide a concise description of the latest developments in the field.

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Identification of Sjögren’s syndrome oral fluid biomarker candidates following high-abundance protein depletion

Abstract: The significance of OF novel depletion methodologies is clearly demonstrated for increased visibility of biomarker candidates as well as for unveiling possible mechanisms involved in this syndrome. This represents a major contribution to our ability to use OF as a future diagnostic fluid.

Cited by 55 publications

References 20 publications

Standardisation of labial salivary gland histopathology in clinical trials in primary Sjögren's syndrome

Standardisation of labial salivary gland histopathology in clinical trials in primary Sjögren's syndrome

Overexpression of the transmembrane carbonic anhydrase isoforms IX and XII in the inflamed synovium

Liquid‐phase based separation systems for depletion, prefractionation, and enrichment of proteins in biological fluids and matrices for in‐depth proteomics analysis—An update covering the period 2014–2016

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