Identification of spinal neurons in the embryonic and larval zebrafish

Bernhardt, Robert R.; Chitnis, Ajay B.; Lindamer, Laurie A.; Kuwada, John Y.

doi:10.1002/cne.903020315

Cited by 305 publications

(360 citation statements)

References 27 publications

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“…Previous studies of zebrafish RB neurons have described peripheral processes in the skin (Metcalfe et al, 1990;Bernhardt et al, 1990) but not in the dorsal caudal fin fold. To learn whether this was a characteristic location of RB innervation, we examined fixed embryos labeled with zn-12 antibody (Metcalfe et al, 1990).…”

Section: Rbs Undergo Degenerative Morphologic Changes Associated Withmentioning

confidence: 90%

“…Because anti-islet1/2 antibody is methanol/acetone sensitive, acetone incubation was omitted. RBs in 3d and older embryos were distinguished from other neurons by size, shape, and position in the dorsal spinal cord (Bernhardt et al, 1990). For double labeling with zn-12 or anti-islet1/2 and TUNEL, embryos were first antibody labeled.…”

Section: Antibody and Tunel Labelingmentioning

confidence: 99%

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Slow degeneration of zebrafish Rohon‐Beard neurons during programmed cell death

Reyes

Haendel

Grant

et al. 2003

Developmental Dynamics

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Rohon-Beard cells are large, mechanosensory neurons located in the dorsal spinal cord of anamniote vertebrates. In most species studied to date, these cells die during development. We followed labeled Rohon-Beard cells in living zebrafish embryos and found that they degenerate slowly, over many days. During degeneration, the soma shrinks and finally disappears, and the processes become beady in appearance and finally break apart, but they do not retract. Zebrafish Rohon-Beard cells apparently fragment their DNA, as revealed by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) labeling, before undergoing degenerative morphologic changes. We also followed the development of labeled dorsal root ganglion neurons, as they are developing at the same stages that Rohon-Beard cells are degenerating. We found that, although axons of both cell types extend into similar regions, Rohon-Beard cells degenerate normally in mutants lacking dorsal root ganglia, providing evidence that interactions between the two cell types are not responsible for Rohon-Beard cell degeneration. Developmental Dynamics 229:30 -41, 2004.

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Section: Rbs Undergo Degenerative Morphologic Changes Associated Withmentioning

confidence: 90%

Section: Antibody and Tunel Labelingmentioning

confidence: 99%

Slow degeneration of zebrafish Rohon‐Beard neurons during programmed cell death

Reyes

Haendel

Grant

et al. 2003

Developmental Dynamics

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“…In the zebrafish spinal cord, commissural axons cross the ventral midline and then follow a contralateral diagonal trajectory that is directed anteriorly and dorsally. Upon reaching the dorsal spinal cord, these axons turn to extend along a longitudinal fiber tract (see Bernhardt et al, 1990;Bernhardt, 1994). We have recently used DiI labeling to re-examine the pathfinding of commissural axons on the contralateral side of the midline in the developing mouse and chick spinal cord.…”

Section: Commissural Axon Pathfinding On the Contralateral Side Of Thmentioning

confidence: 99%

Axon guidance at the midline choice point

Kaprielian

Runko

Imondi

2001

Developmental Dynamics

146

108

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The central nervous system (CNS) of higher organisms is bilaterally-symmetric. The transfer of information between the two sides of the nervous system occurs through commissures formed by neurons that project axons across the midline to the contralateral side of the CNS. Interestingly, these axons cross the midline only once. Other neurons extend axons that never cross the midline; they project exclusively on their own (ipsilateral) side of the CNS. Thus, the midline is an important choice point for several classes of pathfinding axons. Recent studies demonstrate that specialized midline cells play critical roles in regulating the guidance of both crossing and non-crossing axons at the ventral midline of the developing vertebrate spinal cord and the Drosophila ventral nerve cord. For example, these cells secrete attractive cues that guide commissural axons over long distances to the midline of the CNS. Furthermore, shortrange interactions between guidance cues present on the surfaces of midline cells, and their receptors expressed on the surfaces of pathfinding axons, allow commissural axons to cross the midline only once and prevent ipsilaterally-projecting axons from entering the midline. Remarkably, the molecular composition of commissural axon surfaces is dynamically-altered as they cross the midline. Consequently, commissural axons become responsive to repulsive midline guidance cues that they had previously ignored on the ipsilateral side of the midline. Concomitantly, commissural axons lose responsiveness to attractive guidance cues that had initially attracted them to the midline. Thus, these exquisitely regulated guidance systems prevent commissural axons from lingering within the confines of the midline and allow them to pioneer an appropriate pathway on the contralateral side of the CNS. Many aspects of midline guidance are controlled by mechanistically and evolutionarily-conserved ligand-receptor systems. Strikingly, recent studies demonstrate that these receptors are modular; the ectodomains determine ligand recognition and the cytoplasmic domains specify the response of an axon to a given guidance cue. Despite rapid and dramatic progress in elucidating the molecular mechanisms that control midline guidance, many questions remain.

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“…Axons of primary motor neurons that emerge prior to 18 h pf and leave the spinal cord ventrally (Bernhardt et al, 1990;Myers et al, 1986;Pike and Eisen, 1990) are also stained.…”

Section: Expression Of E587 Ag In the Developing Hindbrain And Spinalmentioning

confidence: 99%

Expression of an L1-Related Cell Adhesion Molecule on Developing CNS Fiber Tracts in Zebrafish and Its Functional Contribution to Axon Fasciculation

Weiland

Ott

Bastmeyer

et al. 1997

Molecular and Cellular Neuroscience

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E587 antigen, an L1-related cell adhesion molecule, is expressed by growing axons and has previously been shown to enhance axon growth and to mediate fasciculation of axons from newborn retinal ganglion cells in goldfish. In zebrafish, the monoclonal antibody E17 against E587 antigen stains all axons in the primary tracts and commissures from 17 h postfertilization (pf) onward and axons which are added subsequently to this scaffold. Moreover, Fab fragments of an E587 antiserum (E587 Fabs) injected into the ventricle of 30-h pf zebrafish embryos caused a marked defasciculation of distinct axon bundles in the posterior commissure, in hindbrain commissures, and in longitudinal tracts of the hindbrain, where they also caused increased crossings between fascicles. The regulated expression of E587 antigen by all developing axons and the effects caused by E587 Fabs show that E587 antigen contributes to the formation of tight and orderly fascicles in the developing CNS.

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Identification of spinal neurons in the embryonic and larval zebrafish

Cited by 305 publications

References 27 publications

Slow degeneration of zebrafish Rohon‐Beard neurons during programmed cell death

Slow degeneration of zebrafish Rohon‐Beard neurons during programmed cell death

Axon guidance at the midline choice point

Expression of an L1-Related Cell Adhesion Molecule on Developing CNS Fiber Tracts in Zebrafish and Its Functional Contribution to Axon Fasciculation

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