Identification of splice variant of OsGBF1 in Oryza sativa ssp. indica genotypes under salinity stress

Abstract

G-box-binding factors are plant transcription factors, involved in a wide range of biological processes including abiotic stress responses. In this study, we analyzed the expression of during salt stress in two contrasting spp. genotypes, and . Two-day-old seedlings were exposed to NaCl stress under two different conditions. One set was exposed to 100 mM NaCl before transferring to 250 mM (induction stress), while another set was transferred directly to 250 mM (shock stress). During early induction stress, was up-regulated in when compared to. We cloned full-length from these two genotypes, and analyzed the sequences. Our analysis indicated the presence of transcript variants, which are designated as and variant retained introns, which lead to the generation of premature termination codon. transcript levels were not significantly different at 12-h of induction stress in and. At 24-h of shock stress, was up-regulated in both genotypes and the transcript was more in. Since, and are predicted to be splice variants, we examined expression pattern of , a splicing factor and component of the spliceosome. In induction stress, was up-regulated at 12- and 24-h in when compared to. On the contrary, at 24-h shock stress, was down-regulated in when compared to . It is possible that expression was increased in during induction stress for accurate splicing that could aid in tolerance. This is the first report on splice variant and the variant could have specific functions linked to stress tolerance in rice.

RNA-Seq Analysis Demonstrates Different Strategies Employed by Tiger Nuts (Cyperus esculentus L.) in Response to Drought Stress

“…In this study, the POD, SOD, CAT and APX activities increased more within 12 h of exposure. In line with previous data, these data revealed that 12 h was a suitable sampling time to study the mechanisms involved in responding to drought stress in tiger nuts [ 50 , 57 , 58 ].…”

Comparative Transcriptomics of Rice Genotypes with Contrasting Responses to Nitrogen Stress Reveals Genes Influencing Nitrogen Uptake through the Regulation of Root Architecture

“…The 17 Pokkali-specific DEGs having AS events included transcription factors, stress-related genes, chloroplast precursor genes, and several key enzymes such as BIM2, zinc finger C3H4 type domain containing protein, pentatricopeptide (PPR) repeat containing protein, eukaryotic aspartyl protease domain containing protein, indole-3-glycerol phosphate synthase, isochorismate synthase 1, hydrolases, transferases, dehydrogenases (Table S6). All these results indicate the possible role of AS in regulating gene expressions for stress adaptation [53,54].…”

Analysis of the transcriptome and metabolome reveals phenylpropanoid mechanism in common bean (Phaseolus vulgaris) responding to salt stress at sprout stage

“…Plants encounter salt stress from beginning of germination and salt stress first affects the radicles of plants and the radicles are used to study tissues in several plants under salt stress at sprout stage (Ren et al, 2020), including cucumber (Cucumis sativus L.) (Chang et al, 2010), pakchoi (Brassica chinensis L.) (Ren et al, 2020), and soybean (Glycine max L.) (Xu et al, 2011). 12 h and 24 h are appropriate times for sample tissues under salt stress, which had previously been proved in wheat (Li et al, 2013), soybean (Glycine max L.) (Dinler et al, 2014), and rice (Oryza sativa L.) (Ashwini et al, 2018).…”