Identification of stably expressed QTL for resistance to black shank disease in tobacco (Nicotiana tabacum L.) line Beinhart 1000-1

Zhang, Yusheng; Guo, Xuan; Yan, Xingxing; Ren, Min; Jiang, Caihong; Cheng, Yazeng; Wen, Liuying; Liu, Dan; Yu, Zhang; Sun, Mingming; Feng, Qi Sheng; Yang, Aiguo; Cheng, Lei

doi:10.1016/j.cj.2017.12.002

Cited by 13 publications

(13 citation statements)

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“…The current study investigated the utility of numerous genetic mechanisms for resistance to P. nicotianae in tobacco, including Wz-mediated resistance, which had not previously been examined in China. The results were largely consistent with those reported for these tobacco varieties and varieties with similar resistance mechanisms reported elsewhere, including the USA (Drake et al, 2015), South Africa (van Jaarsveld et al, 2002), and other regions of China (Zhang et al, 2018), indicating that these mechanisms perform reliably regardless of environmental factors, pathogen population structure and regional agricultural practices. Additionally, this research confirms that black shank resistance mechanisms developed in a single tobacco-growing region can have broader applicability to all tobacco-growing regions globally.…”

Section: Discussionsupporting

confidence: 90%

Evaluating diverse systems of tobacco genetic resistance to Phytophthora nicotianae in Yunnan, China

et al. 2019

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Black shank, caused by the soilborne pathogen Phytophthora nicotianae, is one of the most devastating diseases affecting tobacco production in China. The most effective strategy for reducing economic loss from this pathogen is development and use of resistant tobacco varieties. Multiple sources and systems of resistance have been developed in the Western Hemisphere; however, populations of P. nicotianae are variable around the world, including the predominance of different races. Different P. nicotianae isolates may react differently on tobacco plants with different systems of resistance, a possibility that could complicate the breeding of cultivars with resistance that is effective in different tobacco production regions worldwide. The objective of this research was to evaluate an array of tobacco germplasm possessing different systems of genetic resistance to black shank disease in tobacco‐growing regions of Yunnan, China. Resistance types included simply inherited resistance mechanisms introgressed from wild Nicotiana relatives and polygenic partial resistance systems of N. tabacum origin. The loci of Wz exhibited high level resistance to black shank in the five diverse disease environments in Yunnan, China. K326 Php/−Wz/− genotype and Beinhart 1000 exhibited the greatest levels of resistance in both 2015 and 2016. Field observed results for 13 tobacco genotypes were highly correlated with those tested in growth chamber evaluation. These findings suggest that both Wz− and Beinhart 1000‐mediated resistance have important commercial value in flue‐cured tobacco breeding programmes in China. Cultivars developed for black shank resistance in China may also have utility in other tobacco‐growing areas.

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Section: Discussionsupporting

confidence: 90%

Evaluating diverse systems of tobacco genetic resistance to Phytophthora nicotianae in Yunnan, China

et al. 2019

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“…To date, only a few studies involving biparental permanent populations, such as RIL and DH populations, have been reported for QTL mapping. Third, many QTL studies are dependent on traditional molecular markers, such as SSRs, amplified fragment length polymorphisms (AFLPs), and restriction fragment length polymorphisms (RFLPs), which have a lower density than SNPs, resulting in insufficient resolution during genetic mapping (Julio et al, 2006 ; Tan et al, 2012 ; Cheng et al, 2015 ; Sun et al, 2018 ; Zhang et al, 2018 ). In this study, we constructed a high-density SNP linkage map of tobacco based on a BH/XHJ RIL for genome-wide QTL mapping.…”

Section: Discussionmentioning

confidence: 99%

“…For a long time, QTL mapping and the development of markers in tobacco were mainly performed on the basis of simple sequence repeat (SSR) markers (Gholizadeh et al, 2012 ; Tong et al, 2016 ). Several QTL mapping studies of tobacco have been reported, including studies on resistance to diseases (e.g., black shank, brown spot, and powdery mildew) (Stavely et al, 1984 ; Shah et al, 2018 ; Sun et al, 2018 ; Zhang et al, 2018 ) as well as to the yield and quality of tobacco (e.g., agronomic traits and chemical components) (Julio et al, 2006 ; Vijay et al, 2010 ; Tan et al, 2012 ; Cheng et al, 2015 ). However, to date, only a few studies involving QTL mapping of important agronomic traits of tobacco have been reported.…”

Section: Introductionmentioning

confidence: 99%

Identification of QTLs Associated With Agronomic Traits in Tobacco via a Biparental Population and an Eight-Way MAGIC Population

Liu

Yuan²,

Si³

et al. 2022

Front. Plant Sci.

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Agronomic traits such as plant height (PH), leaf number (LN), leaf length (LL), and leaf width (LW), which are closely related to yield and quality, are important in tobacco (Nicotiana tabacum L.). To identify quantitative trait loci (QTLs) associated with agronomic traits in tobacco, 209 recombinant inbred lines (RILs) and 537 multiparent advanced generation intercross (MAGIC) lines were developed. The biparental RIL and MAGIC lines were genotyped using a 430 K single-nucleotide polymorphism (SNP) chip assay, and their agronomic traits were repeatedly evaluated under different conditions. A total of 43 QTLs associated with agronomic traits were identified through a combination of linkage mapping (LM) and association mapping (AM) methods. Among these 43 QTLs, three major QTLs, namely qPH13-3, qPH17-1, and qLW20-1, were repeatedly identified by the use of various genetically diverse populations across different environments. The candidate genes for these major QTLs were subsequently predicted. Validation and utilization of the major QTL qLW20-1 for the improvement of LW in tobacco were investigated. These results could be applied to molecular marker-assisted selection (MAS) for breeding important agronomic traits in tobacco.

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“…Cultivation and selection of disease-resistance cultivars play an important role to control tobacco black shank disease [ 11 ]. Many studies have proven that the incidence of black shank disease was higher in susceptible cultivars than resistance cultivars [ 17 ]. Upon pathogen attack, the plant defense system activates, which helps the plant to limit the invasion.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the expression of differentially expressed proteins and enzymatic activity in response to Phytophthora nicotianae across different flue-cured tobacco cultivars

et al. 2022

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Background Black shank disease caused by Phytophthora nicotianae is a serious threat to flue-cured tobacco production. Whole-plant resistance is characterized by the expression of a number of pathogenesis-related proteins, genes, and the activity of different defense-related enzymes. In this study, we investigated the activity of defense-related enzymes and expression of differentially expressed proteins through the iTRAQ technique across two flue-cured tobacco cultivars, i.e., K326 and Hongda, in response to the black shank pathogen. Results Results showed that the highest disease incidence was recorded in flue-cured tobacco cultivar Hongda compared with K326, which shows that Hongda is more susceptible to P. nicotianae than K326. A total of 4274 differentially expressed proteins were detected at 0 h and after 24 h, 72 h of post-inoculation with P. nicotianae. We found that 17 proteins induced after inoculation with P. nicotianae, including pathogenesis (5), photosynthesis (3), oxidative phosphorylation (6), tricarboxylic acid cycle (1), heat shock (1), and 14–3-3 (1) and were involved in the resistance of flue-cured tobacco against black shank disease. The expression of 5 pathogenesis-related proteins and the activities of defense-related enzymes (PPO, POD, SOD, and MDA) were significantly higher in the leaves of K326 than Hongda after inoculation with P. nicotianae. Conclusion These results provide new molecular insights into flue-cured tobacco responses to P. nicotianae. It is concluded that differences in protein expressions and defense-related enzymes play an important role in developing resistance in flue-cured tobacco cultivars against black shank disease.

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Identification of stably expressed QTL for resistance to black shank disease in tobacco (Nicotiana tabacum L.) line Beinhart 1000-1

Cited by 13 publications

References 34 publications

Evaluating diverse systems of tobacco genetic resistance to Phytophthora nicotianae in Yunnan, China

Evaluating diverse systems of tobacco genetic resistance to Phytophthora nicotianae in Yunnan, China

Identification of QTLs Associated With Agronomic Traits in Tobacco via a Biparental Population and an Eight-Way MAGIC Population

Unraveling the expression of differentially expressed proteins and enzymatic activity in response to Phytophthora nicotianae across different flue-cured tobacco cultivars

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