Identification of standard type cultivars in Chrysanthemum (Dendranthema grandiflorum) using SSR markers

Mekapogu, Manjulatha; Kwon, Oh Keun; Hyun, Do Yoon; Lee, Kyung Jun; Ahn, Myung Suk; Park, Jong Taek; Jung, Jae A

doi:10.1007/s13580-019-00201-0

Cited by 11 publications

(5 citation statements)

References 30 publications

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“…These six SSR markers generated 2 to 6 alleles with an average of 3.5 alleles per locus. The allele number per SSR locus is comparable with other results reporting an average of 2.7 alleles and 5.6 alleles per locus in 11 and 147 varieties of chrysanthemum tested with 7 and 14 SSR markers, respectively [29,30]. The degree of polymorphism (PIC) for the 57 white chrysanthemum cultivars with respect to six SSR markers was an average of 0.53, which is similar to that related in previous reports for chrysanthemum cultivars.…”

Section: Discussionsupporting

confidence: 90%

“…SSR markers have been previously used for the discrimination of cultivated chrysanthemum cultivars [26,27]; Shim et al [28] used 14 SSR markers to assess the genetic characterization of 147 chrysanthemum cultivars. About 11 standard-type chrysanthemum cultivars were discriminated using two SSR markers [29]. Han et al [30] developed the SSR database for chrysanthemum used to discriminate chrysanthemum cultivars.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Genetic Diversity in the White-Colored Variants of Spray-Type Chrysanthemum Cultivars Using SSR Markers

et al. 2023

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Chrysanthemums represent the second most important cut flower after rose on the global commercial market. The phenomenal importance and global popularity of chrysanthemums have attracted breeders’ attention, resulting in the release of vast numbers of cultivars. Identifying these cultivars is crucial to protecting breeders’ intellectual property rights and improving the efficiency of breeding. Distinguishing chrysanthemum genotypes based on their morphological characteristics is challenging as they vary highly within this group, hence requiring the use of efficient molecular markers. In this study, we evaluated the genetic diversity of 57 spray-type chrysanthemum cultivars bearing white, ivory, and cream-colored flowers. A total of six loci were evaluated regarding their polymorphism efficiency across the tested cultivars. Allele numbers ranged from 2 to 6, with a mean of 3.5 alleles per locus. The average polymorphism information content (PIC) was 0.53 for six SSR markers. Cluster analysis of genetic relationships using the UPGMA method showed a genetic distance of 0.31 to 1.00, and the 57 white variants of chrysanthemum cultivars were characterized using the tested SSR markers. However, two sets of cultivars, namely, Pure Angel–Neba and Ladost–White wing, exhibited total genetic similarity and hence could not be discriminated. These results provide efficient SSR markers that can be used to identify chrysanthemum cultivars (and assess their genetic relationships) that cannot be discriminated based on phenotype.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity in the White-Colored Variants of Spray-Type Chrysanthemum Cultivars Using SSR Markers

et al. 2023

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“…Several methods and molecular techniques have been developed to detect genetic diversity within and among cultivars [48][49][50]. The discovery of molecular marker such as SSRs has improved the productivity and accuracy in classical plant breeding, playing a vital role in molecular diversity studies [51][52][53][54][55]. Systematic mango breeding is laborious, time-consuming and is a long-term endeavor (up to 25 years) due to a highly heterozygous genome as well as long juvenility.…”

Section: Discussionmentioning

confidence: 99%

Simple Sequence Repeats-Based DNA Fingerprinting and Varietal Identification of Mango Cultivars

Iqbal,

Ashraf,

Cai

et al. 2023

Preprint

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Assessment of the genetic distinctiveness of a cultivar through morphological descriptors is an important tool for both the registration and the protection. New mango genotypes have been improved using valuable diverse germplasm resources to ensure food security. DNA fingerprinting based simple sequence repeats (SSR)-markers have been the most broadly used, effective and accurate in evaluation of genetic characterization of a cultivar. Molecular breeding is an effective source of genetic gain after improvement of fruit trees using marker assisted genomic selection. Mango (Mangifera indica L.) is an allotetraploid (2n = 4X= 40) drupe fruit and has high nutritional value belongs to genus Mangifera and family Anacardiaceae. Mango cultivars are used with worldwide acceptance to pharmacological, ethnomedical, and phytochemical industries. This study investigated the molecular evaluation of a new mango cultivar ‘Azeem Chaunsa’ using a set of the most effective 50 hyper-variable polymorphic SSR markers. Highly specific DNA fingerprints were identified in the genome of this mango cultivar, ‘Azeem Chaunsa’ compared with three standard cultivars such as Sindhri, Samar Bahisht (S.B) Chaunsa and Sufaid Chaunsa. Our results showed that SSR markers could efficiently assess genetic diversity in mango. An agglomerative hierarchical clustering method was used to construct dendrogram based on the ‘Unweighted Pair- Group Method with Arithmetic Mean’ (UPGMA). The genetic similarity coefficients were recorded between the mangos cultivars ranged from 0.49 to 0.67. Cultivar identification (CID) evaluates association among standard cultivars and Azeem Chaunsa and further concludes significant variations. CID results concluded that cultivar ‘Azeem Chaunsa’ varied significantly from the check cultivar, Sindhri (46.2%), S.B Chaunsa (45%) and Sufaid Chaunsa (46.7%). The results obtained in this study will orient cultivar identification strategies for a successful future mango breeding programmes in the context of climate change.

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“…In chrysanthemum, cultivar propagation is primarily achieved by vegetative cuttings and suckers. Considerable inbuilt genetic variation confers the advantage of easy manipulation by vegetative propagation methods, resulting in a diverse array of morphological variations [ 16 ]. Since chrysanthemum is an important cut flower, breeding is mainly focused on aesthetic characteristics, including floral color and shape.…”

Section: Importance Of Floral Attributesmentioning

confidence: 99%

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Mekapogu

Kwon

Song

et al. 2022

IJMS

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Incessant development and introduction of novel cultivars with improved floral attributes are vital in the dynamic ornamental industry. Chrysanthemum (Chrysanthemum morifolium) is a highly favored ornamental plant, ranking second globally in the cut flower trade, after rose. Development of new chrysanthemum cultivars with improved and innovative modifications in ornamental attributes, including floral color, shape, plant architecture, flowering time, enhanced shelf life, and biotic and abiotic stress tolerance, is a major goal in chrysanthemum breeding. Despite being an economically important ornamental plant, the application of conventional and molecular breeding approaches to various key traits of chrysanthemum is hindered owing to its genomic complexity, heterozygosity, and limited gene pool availability. Although classical breeding of chrysanthemum has resulted in the development of several hundreds of cultivars with various morphological variations, the genetic and transcriptional control of various important ornamental traits remains unclear. The coveted blue colored flowers of chrysanthemums cannot be achieved through conventional breeding and mutation breeding due to technical limitations. However, blue-hued flower has been developed by genetic engineering, and transgenic molecular breeding has been successfully employed, leading to substantial progress in improving various traits. The recent availability of whole-genome sequences of chrysanthemum offers a platform to extensively employ MAS to identify a large number of markers for QTL mapping, and GWAS to dissect the genetic control of complex traits. The combination of NGS, multi-omic platforms, and genome editing technologies has provided a tremendous scope to decipher the molecular and regulatory mechanisms. However, the application and integration of these technologies remain inadequate for chrysanthemum. This review, therefore, details the significance of floral attributes, describes the efforts of recent advancements, and highlights the possibilities for future application towards the improvement of crucial ornamental traits in the globally popular chrysanthemum plant.

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Identification of standard type cultivars in Chrysanthemum (Dendranthema grandiflorum) using SSR markers

Cited by 11 publications

References 30 publications

Assessment of Genetic Diversity in the White-Colored Variants of Spray-Type Chrysanthemum Cultivars Using SSR Markers

Assessment of Genetic Diversity in the White-Colored Variants of Spray-Type Chrysanthemum Cultivars Using SSR Markers

Simple Sequence Repeats-Based DNA Fingerprinting and Varietal Identification of Mango Cultivars

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

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