Identification of staphylococci by the API staph, sceptor, rosco and simplified lyogroup systems

Pascoli, L; Chiaradia, V.; Mucignat, Giorgio; Santini, G

doi:10.1007/bf02013298

Cited by 5 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, various methods for species typing of CNS isolated from blood cultures were compared. Two commercial kits were used for species typing: APT Staph@ (4, 10) and Staphzyme@ (23,28). Identification of S. epidermidis and S. huemolyticus by the use of new developed PCR methods was evaluated, as well as the role of some simple phenotypic tests.…”

Section: Discussionmentioning

confidence: 99%

Comparison of genotypic and phenotypic methods for species identification of coagulase‐negative staphylococcal isolates from blood cultures

Hedin

1994

APMIS

View full text Add to dashboard Cite

Coagulase‐negative staphylococci are often isolated from blood cultures. Simple methods are needed for correct identification of those species most frequently found. In this study, PCR methods were developed for the identification of S. epidermidis and S. haemolyticus, based on DNA sequences in their 16S rDNA. The results obtained by these methods were compared with those obtained using a number of phenotypic methods, including two commercial kits API Staph® and Staphzyme®. Fifteen type collection strains and 133 blood culture isolates were tested. The sensitivity of the PCR for identification of S. epidermidis was 99% compared with API Staph®, but the specificity was lower, 94%, because of positive results also for S. capitis. The results by the PCR for identification of S. haemolyticus correlated closely with the Staphzyme® results, 13 isolates being identified by Staphzyme® and 16 by the PCR. API Staph®, however, identified only four clinical isolates as S. haemolyticus, probably too few. Among the individual phenotypic tests performed, a trehalose‐mannitol agar method and a desferrioxamine disc diffusion test for the identification of S. epidermidis were found to be very accurate. Anaerobic growth after overnight incubation could be used to distinguish S. epidermidis from S. hominis. The conclusion is that a majority of all Gram‐positive, catalase‐positive and coagulase‐negative blood culture isolates can be typed as regards species level using only a few genotypic and/or phenotypic tests.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of genotypic and phenotypic methods for species identification of coagulase‐negative staphylococcal isolates from blood cultures

Hedin

1994

APMIS

View full text Add to dashboard Cite

show abstract

“…API staph (Analytical Profile Index for identification of the Genus Staphylococcus) is a standardized system for the identification of the Genera: Staphylococcus, Micrococcus and Kocuria, which uses miniaturized biochemical tests and specially adapted database. It was used according to [13] to identify gram positive bacteria.…”

Section: Identification Of Staphylococcal Isolates Using Api Staph (B...mentioning

confidence: 99%

Investigation of S. Aureus in Butter and Ghee in Some Markets in Nyala Locality, South Dafour State, Sudan

Khalid¹,

Abubaker²

2022

Int. J. Res. Publ. Rev.

View full text Add to dashboard Cite

This study was aiming at investigating S. aureus in butter and ghee in Nyala city in South Darfour State during the year 2021. A total of 80 milk products' samples (40 butter and 40 ghee) were collected from 4 markets (Nyala, El Ban gadeed, Manawashy and El Laloba) in Nyala Locality. Samples were examined bacteriologically using conventional and rapid API methods. All butter samples (100%) collected from El Laloba and Manawashy markets were positive for S. aureus. Eighty percent of the samples collected from Nyala market were positive for S. aureus. Sixty percent of the samples collected from El Ban gadeed market were positive for S. aureus. All ghee samples (100%) collected from El Laloba and El Bangadeed markets were negative for S. aureus. Sixty percent of the samples collected from Nyala and Manawashy markets were positive for S. aureus. The prevalence of S. aureus in butter samples in Nyala Locality was 85%. The prevalence of S. aureus in butter samples in Nyala Locality was 30%. Butter in Nyala Locality is highly contaminated and ghee is less contaminated with S. aureus. Pasteurization and heat treatment are highly recommended before processing of milk.

show abstract

“…France) (3,9). The Malmo isolates were identified as S. epidrrnlidis using Staphzyme ' (Rosco Diagnostica, Taastrup, Denmark) (21,32).…”

Section: Bucteriul Struinsmentioning

confidence: 99%

Detecting methicillin‐resistant Staphylococcus epidermidis– disc diffusion, broth breakpoint or polymerase chain reaction?

Hedin¹,

Löfdahl²

1993

APMIS

View full text Add to dashboard Cite

Lofdahl. S. Detecting methicillin-resistant Staphylococcus epidermidis ~ disc diffusion, broth breakpoint or polymerase chain reaction? APMIS 101: 31 1-318, 1993.Growth conditions are important for the expression of resistance to methicillin among staphylococci. Consequently a phenotypic susceptibility test has to be chosen carefully to avoid false susceptible results. In this study we wanted to devise rapid and simple phenotypic tests whose results completely correlate with the presence of the methicillin resistance gene, mecA. A simplified polymerase chain reaction (PCR) method not needing separate DNA extraction from the tested bacteria was used to amplify a 449 bp region of the mecA gene. One hundred and ten strains of S. epidermidis were tested. The results were in complete agreement with those from a broth tube breakpoint test, known to identify more strains as resistant than does the method recommended by NCCLS. In disc diffusion tests it was possible to clearly distinguish resistant from susceptible strains by using discs containing oxacillin, cephalexin and cephradine. A 5 pg cephradine disc was further analysed by testing another 441 consecutive clinical isolates of staphylococci. All resistant coagulase-negative staphylococci grew out to the edge of this disc, whereas susceptible strains showed an inhibition zone at least 10 mm in diameter. The 5 pg cephradine disc is recommended for routine work. The PCR method and broth tube breakpoint test are both reliable reference methods.

show abstract

Identification of staphylococci by the API staph, sceptor, rosco and simplified lyogroup systems

Cited by 5 publications

References 12 publications

Comparison of genotypic and phenotypic methods for species identification of coagulase‐negative staphylococcal isolates from blood cultures

Comparison of genotypic and phenotypic methods for species identification of coagulase‐negative staphylococcal isolates from blood cultures

Investigation of S. Aureus in Butter and Ghee in Some Markets in Nyala Locality, South Dafour State, Sudan

Detecting methicillin‐resistant Staphylococcus epidermidis– disc diffusion, broth breakpoint or polymerase chain reaction?

Contact Info

Product

Resources

About