Identification of Staphylococcus aureus enterotoxins A and B genes by PCR-ELISA

Enterotoxigenic potential of Staphylococcus aureus isolated from Artisan Minas cheese from the Serra da Canastra - MG, Brazil

“…According to Gilligan et al (2000), techniques that use commercial diagnostic kits based on enzymatic immunoassay, as well as OSP and radioimmunoassays are widely used for the staphylococcal enterotoxin detection. However, sensitivity and specificity may vary depending on the purity of the reagents and the level of toxin expression.…”

Detection of the enterotoxigenic genes (sei,sej) in Staphylococcus aureus isolates from bovine mastitis milk in the West Azerbaijan of Iran

“…In addition, the detection limit and specificity of these methods always depend on having detectable amounts of toxins and may vary significantly according to reagent purity. Since DNA sequence information is available for all described SEs, DNA–DNA hybridization and PCR now offer alternative methods for detection of these genes (Gilligan et al 2000; Johnson et al 1991; McLauchlin et al 2000; Omoe et al 2002). Because of the importance of the enterotoxins on public health and great geographical variation in the distribution of enterotoxigenic strains, in this study, the distribution of the genes that encode SEJ and SEI was analyzed by PCR in S. aureus isolated from bovine mastitis.…”

Real-time Fluorogenic PCR Assays for the Detection of entA, the Gene Encoding Staphylococcal Enterotoxin A

“…DRn, change in fluorescence agarose gel electrophoresis to analyze the results of PCR. An innovative ELISA assay was used by Gilligan et al (2000) to detect amplified fragments of entA and entB. Digoxigenin-dUTP was used during amplification to label the amplified DNA.…”