1989
DOI: 10.1016/s0741-5214(89)70037-9
|View full text |Cite
|
Sign up to set email alerts
|

Identification of Staphylococcus epidermidis vascular graft infections: A comparison of culture techniques

Abstract: Culture of prosthetic material is routinely used to exclude or implicate infection in the pathogenesis of late-appearing graft complications. In a canine model of aortic graft infection caused by a bacterial biofilm, the influence of ~lture media (blood agar and tryptic soy broth) and mechanical surface biofilm disruption (tissue grinding and ultrasonic oscillation) on microorganism recovery was determined. Dacron prostheses colonized in vitro with Staphylococcus epidermidis were implanted in the infrarenal ao… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
23
0
1

Year Published

1990
1990
2016
2016

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 29 publications
(24 citation statements)
references
References 13 publications
0
23
0
1
Order By: Relevance
“…schleifeni was isolated in a series of nine blood cultures taken over a period of 15 days (146). To minimize the risk of false-negative cultures in a CNS infection of a prosthetic device, ultrasonic oscillation may be used to shake off from prosthetic surfaces adherent organisms embedded in a biofilm matrix (25,301). Methods that used ultrasonic oscillation of explanted vascular graft material demonstrated a significant increase in the incidence of cultures positive for S. epidermidis compared with standard blood agar plate and broth culture techniques.…”
Section: Specimen Collection and Processingmentioning
confidence: 99%
“…schleifeni was isolated in a series of nine blood cultures taken over a period of 15 days (146). To minimize the risk of false-negative cultures in a CNS infection of a prosthetic device, ultrasonic oscillation may be used to shake off from prosthetic surfaces adherent organisms embedded in a biofilm matrix (25,301). Methods that used ultrasonic oscillation of explanted vascular graft material demonstrated a significant increase in the incidence of cultures positive for S. epidermidis compared with standard blood agar plate and broth culture techniques.…”
Section: Specimen Collection and Processingmentioning
confidence: 99%
“…However, this bias is the result of the clinical reality, since the omission of antibiotic therapy could adversely affect patients and this issue is related to each study predicting AGI. Moreover, routine microbiologic techniques can sometimes fail to isolate the microorganism from perigraft material [35,36]. Sonication techniques have been described to identify indolent gram-positive microorganisms by using ultrasound energy to agitate particles from the graft sample for microbiology [35,36].…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, routine microbiologic techniques can sometimes fail to isolate the microorganism from perigraft material [35,36]. Sonication techniques have been described to identify indolent gram-positive microorganisms by using ultrasound energy to agitate particles from the graft sample for microbiology [35,36]. However, we did not apply these sonication techniques in this retrospective study because they were not available in our hospital.…”
Section: Discussionmentioning
confidence: 99%
“…Many authors report that Coagulase-negative Staphylococcus is one of the microorganisms most often responsible for hospital infections 14 and especially so with relation to surgical site infections after vascular surgery. 5,6,9,[31][32][33][34][35][36] It is also associated with high rates of antimicrobial resistance. 32,34 Our data confirmed a high prevalence of Coagulase-negative Staphylococcus (28.6% overall), but this pathogen was significantly more prevalent among the non-resistant group (50% vs. 17.4%).…”
Section: Discussionmentioning
confidence: 99%