Identification of surface-associated proteins in the probiotic bacterium Lactobacillus rhamnosus GG

Sánchez, Borja; Bressollier, Philippe; Chaignepain, Sthéphane; Schmitter, Jean‐Marie; Urdaci, María C.

doi:10.1016/j.idairyj.2008.09.005

Cited by 31 publications

(35 citation statements)

References 31 publications

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“…For this purpose, two species were selected. The first one was l. rhamnosus GG, which is a widely studied bacterium because of its claimed probiotic effect on the human health (Servin 2004;Saxelin et al 2005;Sánchez et al 2009). The second one was l. paracasei subsp.…”

Section: Effect Of Lab On the Surface Growth Of G Candidummentioning

confidence: 99%

Effect of temperature and lactic acid bacteria on the surface growth of Geotrichum candidum

Hudecová¹,

Valı́k²,

Liptáková³

et al. 2011

Czech J. Food Sci.

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Hudecová A., Valík Ľ., Liptáková D., Pelikánová J., Čižniar M. (2011): Effect of temperature and lactic acid bacteria on the surface growth of Geotrichum candidum, Czech J. Food Sci., 29 (Special Issue): S61-S68.The surface growth of Geotrichum candidum isolated from ewes' lump cheese was studied on pure agar medium and that inoculated with lactobacillus rhamnosus GG and l. paracasei subsp. paracasei CCM 1753. The colony growth rates of fungus calculated from the growth curves were modelled in relation to temperature by the cardinal temperature model with inflection (CTMI). The following cardinal values resulted from the secondary model: t min = -3°C, t opt = 27.6°C, and t max = 35.4°C and optimal colony growth rate µ opt = 5.34 mm/day. A quantitative study also showed that the simultaneous growth of l. rhamnosus GG and l. paracasei subsp. paracasei CCM 1753 had either no or only a slight effect on the fungal growth rates, respectively. These results pointed out that other intrinsic or extrinsic factors should be applied for the protection of fresh cheeses against the undesirable growth of G. candidum.

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Section: Effect Of Lab On the Surface Growth Of G Candidummentioning

confidence: 99%

Effect of temperature and lactic acid bacteria on the surface growth of Geotrichum candidum

Hudecová¹,

Valı́k²,

Liptáková³

et al. 2011

Czech J. Food Sci.

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“…It has been proposed that cell surface proteins are involved in cell protection and surface recognition and that they could be potential mediators in the initial steps involved in autoaggregation and adhesion [8][9][10]. In cell envelope proteome studies of potentially probiotic bacteria, the cell wall protein fraction has typically been extracted by lysozyme-containing buffer [11][12][13][14][15][16], lithium chloride [16,17], or some other cell surface molecule or proteinsolubilizing agent [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Cell surface proteins play an important role in probiotic activities of Lactobacillus reuteri

Singh

Malik

Kaur

2016

Nutrire

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Background: Eight Lactobacillus reuteri strains, previously isolated from breast-fed human infant feces, were selected to assess the potential contribution of their surface proteins in probiotic activity. These strains were treated with 5 M LiCl to remove their surface proteins, and their tolerance to simulated stomach-duodenum passage, cell surface characteristics, autoaggregation, adhesion, and inhibition of pathogen adhesion to Caco-2 cells were compared with untreated strains.

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“…Although the main functions of these proteins reside in the cytosol, their surface localization was already reported earlier [Deepika et al, 2012;Saad et al, 2009]. This 'aberrant' localization might point towards a moonlighting role for these proteins [Deepika et al, 2012;Izquierdo et al, 2009;Jeffery, 2003;Sanchez et al, 2009a], which has already been documented in other species for the glycolytic enzymes Eno, GapA, and Pgk [Castaldo et al, 2009;Granato et al, 1999;Henderson, 2014;Kinoshita et al, 2008a, b;Sanchez et al, 2009a]. In total, we identified 15 putative cytosolic glycoproteins, 28 glycoproteins in the CWCM fraction, 17 CWA proteins, and 5 SP ( table 1 ).…”

Section: Proteome Fractionationmentioning

confidence: 80%

“…Next to their glycolytic activity, these enzymes also play a role in binding to the extracellular environment [Castaldo et al, 2009;Granato et al, 1999;Henderson, 2014;Kinoshita et al, 2008a, b;Sanchez et al, 2009a]. These proteins have potentially also a moonlighting role in L. rhamnosus GG as many observations were made in closely related lactobacilli [Castaldo et al, 2009;Granato et al, 1999;Kinoshita et al, 2008a, b].…”

Section: General Trends In Protein Glycosylationmentioning

confidence: 99%

Systematic Exploration of the Glycoproteome of the Beneficial Gut Isolate <b><i>Lactobacillus rhamnosus </i></b>GG

Tytgat

Schoofs²,

Vanderleyden³

et al. 2016

Microb Physiol

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Glycoproteins form an interesting class of macromolecules involved in bacterial-host interactions, but they are not yet widely explored in Gram-positive and beneficial species. Here, an integrated and widely applicable approach was followed to identify putative bacterial glycoproteins, combining proteome fractionation with 2D protein and glycostained gels and lectin blots. This approach was validated for the microbiota isolate Lactobacillus rhamnosus GG. The approach resulted in a list of putative glycosylated proteins receiving a ‘glycosylation score'. Ultimately, we could identify 41 unique glycosylated proteins in L. rhamnosus GG (6 top-confidence, 10 high-confidence and 25 putative hits; classification based on glycosylation score). Most glycoproteins are associated with the cell wall and membrane. Identified glycoproteins include proteins involved in transport, translation, and sugar metabolism processes. A robust screening resulted in a comprehensive mapping of glycoproteins in L. rhamnosus GG. Our results reflect the glycosylation of sugar metabolism enzymes, transporters, and other proteins crucial for cell physiology. We hypothesize that protein glycosylation can confer an extra level of regulation, for example by affecting enzyme functions. This is the first systematic study of the glycoproteome of a probiotic and beneficial gut isolate.

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Identification of surface-associated proteins in the probiotic bacterium Lactobacillus rhamnosus GG

Cited by 31 publications

References 31 publications

Effect of temperature and lactic acid bacteria on the surface growth of Geotrichum candidum

Effect of temperature and lactic acid bacteria on the surface growth of Geotrichum candidum

Cell surface proteins play an important role in probiotic activities of Lactobacillus reuteri

Systematic Exploration of the Glycoproteome of the Beneficial Gut Isolate <b><i>Lactobacillus rhamnosus </i></b>GG

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