Identification of T cell lymphoma tumor antigens on human T cell lines

Kaplan, Joseph; Tilton, Jerel; Peterson, Ward D.

doi:10.1002/ajh.2830010206

Cited by 70 publications

(29 citation statements)

References 7 publications

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“…TCGF was prepared from a high-producer subclone (J6.8.9. 15) of the human leukemia T-cell line, JURKAT (15). The cells (4 X 106 cells per ml) were stimulated in serum-free Dulbecco's modified Eagle's medium with 1.5 Ag of purified phytohemagglutinin (PHA; HA-16, Wellcome Reagents) and 50 ng of phorbol 12-myristate 13-acetate (PMA; Consolidated Midland, Brewster, NY) per ml.…”

Section: Methodsmentioning

confidence: 99%

Purification and partial sequence analysis of human T-cell growth factor.

Robb¹,

Kutny²,

Chowdhry³

1983

Proc. Natl. Acad. Sci. U.S.A.

117

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A murine monoclonal antibody directed against human T-cell growth factor (TCGF) from the JURKAT cell line was used for affinity column.purification of the factor. Bound TCGF was eluted nearly quantitatively at low pH, and the recovered factor appeared homogeneous by two-dimensional gel electrophoresis. The molecule is markedly hydrophobic, with a high content of leucine. A single NH2-terminal sequence of 36 residues was obtained by automated Edman degradation, further supporting the homogeneity of the material. Thus, significant quantities of purified TCGF have been prepared in a single step, making possible detailed analysis of its molecular structure and biological role.

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Section: Methodsmentioning

confidence: 99%

Purification and partial sequence analysis of human T-cell growth factor.

Robb¹,

Kutny²,

Chowdhry³

1983

Proc. Natl. Acad. Sci. U.S.A.

117

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“…A modification of the RACE (rapid amplification of cDNA ends) technique (14) was used to clone the 5' end of SIL. Total cellular RNA (10 ,ug) from the T-cell line Jurkat (16) Nucleotide sequence accession number. The nucleotide sequence of the composite SIL cDNA (see Fig.…”

Section: Methodsmentioning

confidence: 99%

Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia.

1991

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The SIL (SCL interrupting locus) gene was initially discovered at the site of a genomic rearrangement in a T-cell acute lymphoblastic leukemia cell line. This rearrangement, which occurs in a remarkably site-specific fashion, is present in the leukemic cells of 16 to 26% of patients with T-cell acute lymphoblastic leukemia. We have now cloned a normal SIL cDNA from a cell line which does not carry the rearrangement. The SIL cDNA has a long open reading frame of 1,287 amino acids, with a predicted molecular size of 143 kDa. The predicted protein is not homologous with any previously described protein; however, a potential eukaryotic topoisomerase I active site was identified. Cross-species hybridization using a SIL cDNA probe indicated that the SIL gene was conserved in mammals. A survey of human and murine cell lines and tissues demonstrated SIL mRNA to be ubiquitously expressed, at low levels, in hematopoietic cell lines and tissues. With the exception of 11.5-day-old mouse embryos, SIL mRNA was not detected in nonhematopoietic tissues. The genomic structure of SIL was also analyzed. The gene consists of 18 exons distributed over 70 kb, with the 5' portion of the gene demonstrating alternate exon utilization.Nonrandom chromosomal translocations or deletions present in specific malignancies have been recognized for the past 30 years, since the association of the Ph chromosome with chronic myelogenous leukemia (20). The analysis of these recurring chromosomal abnormalities at a molecular level has identified numerous proto-oncogenes and growthaffecting genes (for a review, see reference 23). These rearrangements often occur at the sites of transcriptionally active DNA, which may be in a more open chromatin configuration and thus more susceptible to chromosomal breakage and rejoining (17). Chromosomal breakpoints often involve genes that are important for the growth or development of the cell that undergoes the translocation; the classic example is seen in Burkitt's lymphoma, in which the c-myc and immunoglobulin genes are disrupted and brought into chromosomal contiguity (18).Traditionally, these chromosomal abnormalities have been identified cytogenetically on preparations of metaphase chromosomes. Recently, while investigating the genomic structure of the newly described SCL (TCL5, tal-J) gene (6, 7, 9, 13), a member of the basic helix-loop-helix family of transcription factors, we identified a frequent, site-specific chromosomal deletion that involved SCL and a previously unidentified locus that we called SIL (for SCL interrupting locus) (4). This interstitial deletion is the first known instance whereby two genes, neither one of which is an antigen receptor gene, are joined through the action of the V(D)J recombinase system. The deletion occurs below the level of conventional cytogenetic detection and leads to a fusion mRNA between SIL and SCL. A 5.5-kb SIL transcript, distinct from SCL, was identified in normal tissues by Northern (RNA) blot hybridization (4). To better understand how disruption of the SIL g...

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“…MT-2 is a HTLV-infected T-cell line of Japanese origin (3). Uninfected T-cell lines used as controls included 8402 (18), , and Jurkat (20). NC 37 is a B lymphoid line (21), and HL 60 is of myeloid origin (22).…”

Section: Methodsmentioning

confidence: 99%

“…NC 37 is a B lymphoid line (21), and HL 60 is of myeloid origin (22). All were cultured according to conditions described (1,3,7,(17)(18)(19)(20)(21)(22).…”

Section: Methodsmentioning

confidence: 99%

Human T-cell leukemia virus-associated membrane antigens: identity of the major antigens recognized after virus infection.

Lee¹,

Coligan²,

Homma³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

103

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Specific antibodies to cell membrane antigens found on human T-cell leukemia virus (HTLV)-infected cells have been detected in Japanese patients with adult T-cell leukemia/lymphoma and in asymptomatic carriers, using a live cell-membrane immunofluorescence assay. Reactivity of the positive antisera was analyzed using radioimmunoprecipitation and NaDodSO4/PAGE with the HTLV-infected tumor cell line Hut 102 (clone B2). The major cell-associated antigens identified include two glycoproteins of -61 and 45 kDa, which appear to be the most immunogenic species in exposed people, a nonglycosylated species of 42 kDa, and four additional species that contain gag gene-encoded antigens with sizes ranging from 19 to 55 kDa. The two glycoproteins (gp6l and gp45) are encoded, at least in part, by the env gene of HTLV as evidenced by amino acid sequence analysis.

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Identification of T cell lymphoma tumor antigens on human T cell lines

Cited by 70 publications

References 7 publications

Purification and partial sequence analysis of human T-cell growth factor.

Purification and partial sequence analysis of human T-cell growth factor.

Structural characterization of SIL, a gene frequently disrupted in T-cell acute lymphoblastic leukemia.

Human T-cell leukemia virus-associated membrane antigens: identity of the major antigens recognized after virus infection.

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