Identification of target proteins of N‐acetylglucosaminyl‐transferase V and fucosyltransferase 8 in human gastric tissues by glycomic approach

Abstract

To understand better the mechanism underlying gastric cancer and search for potential markers for gastric cancer prognosis, the proteomic analysis of gastric cancer tissues was conducted using two-dimensional gel electrophoresis and lectin blot, followed by electrospray ionization-mass spectrometry. These approaches permitted identification of glyco- or putative glycosylated proteins which may be associated with tumorigenesis. The proteins identified include molecules involved in sugar metabolism, signal transduction, proteolysis, and stress, as well as several unknown proteins, which were aberrantly glycosylated as evidenced by the L-phytohemagglutinin blot.

Functional Proteomics Study Reveals That N-Acetylglucosaminyltransferase V Reinforces the Invasive/Metastatic Potential of Colon Cancer through Aberrant Glycosylation on Tissue Inhibitor of Metalloproteinase-

“…From the media protein samples were prepared and subjected to twodimensional electrophoretic analysis as described previously (12). For comparison of the expression level, 50 g of proteins was minimally labeled with cy3-or cy5-fluorescent dyes following two-dimensional differential in-gel electrophoresis, according to the manufacturer's instructions.…”

“…For this, we adopted proteomics and glycomics techniques in which two-dimensional electrophoresis, lectin blot analysis, and MS-based protein identification were linked. We have previously reported that these approaches allowed us to identify several candidate proteins that are assumed to be involved in progressions of gastric cancer (12) and colon cancer (13) and have validated these approaches for discovery of biomarker by showing the role of aberrant glycosylation of protein tyrosine phosphatase as an example in cancer cell migration (13). In this article, we report that additional candidate proteins were identified including tissue inhibitor of metalloproteinase-1 (TIMP-1) as the target for GnT-V and, more importantly, that the aberrant glycosylation of TIMP-1 is closely correlated with invasive/metastatic potential of colon cancer cell WiDr.…”

Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers

“…2-DE, dating back to the 1970s [10,11] , in conjunction with MS, has allowed the identification of differentially expressed proteins in various diseases, including GC [12][13][14][15][16][17] . However, the process of translating basic proteomic discoveries to the clinic is very time consuming and expensive, and despite all the efforts put into biomarker research, very few diagnostic protein biomarkers have been approved by the United States Food and Drug Administration [8] .…”

Glycoproteomic analysis of plasma from patients with atopic dermatitis: CD5L and ApoE as potential biomarkers

“…Glycoproteome is also a major subproteome: many clinical biomarkers and therapeutic targets are glycoproteins. In particular, the glycosylation patterns are influenced considerably by the physiological status (Kim et al, 2004;Zhao et al, 2006). Therefore, in this study, a differential glycoprotein profiling of plasma trials of healthy donors and AD patients was performed.…”