Identification of ten corticosteroids in human hair by liquid chromatography–ionspray mass spectrometry

Cirimele, Vincent; Kintz, Pascal; Dumestre, V.; Goulle, J.; Ludes, Bertrand

doi:10.1016/s0379-0738(99)00180-2

Cited by 160 publications

(89 citation statements)

References 5 publications

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“…19 It has recently become feasible to measure cortisol levels in hair over time. 20 This new biomarker could be valuable in assessing prolonged stress. Because hair grows ∼1 cm per month, 21 and cortisol accumulates in hair, it is assumed that cortisol concentrations in hair reflect the longterm mean concentration, similar to hemoglobin A1c for diabetes.…”

Section: Discussionmentioning

confidence: 99%

Maternal Influence on Child HPA Axis: A Prospective Study of Cortisol Levels in Hair

Karlén¹,

Frostell²,

Theodorsson³

et al. 2013

Pediatrics

120

112

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WHAT'S KNOWN ON THIS SUBJECT: Stress affects health of children, potentially persisting as a trajectory into adulthood. Earlier biological markers assess only momentary stress, making it difficult to investigate stress over longer periods of time. Cortisol in hair is a new biomarker of prolonged stress. WHAT THIS STUDY ADDS:Mother and child hair cortisol association suggests a heritable part or maternal calibration. Cortisol output gradually stabilizes, has a stable trait, and is positively correlated to birth weight. Hair cortisol is a promising noninvasive biomarker of prolonged stress, especially applicable for children. abstract OBJECTIVE: To investigate cortisol concentrations in hair as biomarker of prolonged stress in young children and their mothers and the relation to perinatal and sociodemographic factors.METHODS: Prospective cohort study of 100 All Babies In Southeast Sweden study participants with repeated measures at 1, 3, 5, and 8 years and their mothers during pregnancy. Prolonged stress levels were assessed through cortisol in hair. A questionnaire covered perinatal and sociodemographic factors during the child' s first year of life. RESULTS:Maternal hair cortisol during the second and third trimester and child hair cortisol at year 1 and 3 correlated. Child cortisol in hair levels decreased over time and correlated to each succeeding age, between years 1 and 3 (r = 0.30, P = .002), 3 and 5 (r = 0.39, P , .001), and 5 and 8 (r = 0.44, P , .001). Repeated measures gave a significant linear association over time (P , .001). There was an association between high levels of hair cortisol and birth weight (b = .224, P = .020), nonappropriate size for gestational age (b = .231, P = .017), and living in an apartment compared with a house (b = .200, P = .049). In addition, we found high levels of cortisol in hair related to other factors associated with psychosocial stress exposure. CONCLUSIONS:Correlation between hair cortisol levels in mothers and their children suggests a heritable trait or maternal calibration of the child' s hypothalamic-pituitary-adrenocortical axis. Cortisol output gradually stabilizes and seems to have a stable trait. Cortisol concentration in hair has the potential to become a biomarker of prolonged stress, especially applicable as a noninvasive method when studying how stress influences children' s health. Pediatrics 2013;132:e1333-e1340

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Section: Discussionmentioning

confidence: 99%

Maternal Influence on Child HPA Axis: A Prospective Study of Cortisol Levels in Hair

Karlén¹,

Frostell²,

Theodorsson³

et al. 2013

Pediatrics

120

112

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“…Although traditionally cortisol concentrations have been determined in plasma or serum, their determination in other fluids or organic tissues (saliva, milk, muscle, urine, faeces and hair) can be of interest in animal welfare studies of different species (Cooper et al, 1989;Fritsche and Steinhart, 1998;Verkerk et al, 1998;Cirimele et al, 1999;Fritsche et al, 1999;Antignac et al, 2000;Morrow, 2000;Palme et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Validation of an EIA [enzyme immunoassay] technique for the determination of salivary cortisol in cattle

Pérez

Laita

Portal

et al. 2004

Span. j. agric. res.

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This work involves the development and validation of an enzyme immunoassay technique (EIA) for the measurement of the cortisol concentration in cattle saliva. Saliva samples present several advantages over plasma samples in animal welfare studies. Saliva collection avoids venipuncture as a stress factor. Also, saliva components do not affect EIA as plasma components do. At present, there is no validated commercial method for saliva cortisol determination in cattle. Commercially available radioimmunoassay kits for human plasma (detection range: 10-100 ng ml -1 ) are not sensitive enough for animals with low concentrations of salivary cortisol (< 4 ng ml -1 ). Thus, EIA is the method of choice in cattle. Sensitivity, specificity, precision and accuracy EIA tests showed this method to be suitable and reliable. The detection limit was found to be 0.024 ng ml -1 , representing an improvement on previously described techniques. Intra-assay and inter-assay variation coefficients were 1.47-7.30% and 2.40-9.78%, respectively. The recovery rates for cortisol added to saliva samples were 91.36-126.5%. Parallelism tests showed that saliva cortisol levels can be determined in cattle samples without extraction. The correlation between saliva and plasma cortisol was positive (r = 0.75) and the saliva/plasma cortisol ratio was around 10%. Therefore, saliva samples are a suitable alternative to plasma samples in bovine HPA (hypothalamic-pituitary-adrenal) axis evaluation.Key words: immunoassay, HPA axis, cow, stress. ResumenValidación de una técnica de EIA para la determinación de cortisol en saliva de ganado bovino En este trabajo se desarrolla y valida una técnica de enzimoinmunoensayo (EIA) para la determinación directa de cortisol en muestras de saliva de ganado vacuno. La saliva como muestra tiene gran importancia en los estudios de bienestar animal, ya que presenta ventajas respecto al plasma, como son el menor estrés al que se somete a los animales para su obtención y la menor interferencia que producen los componentes de la saliva en la determinación directa en el EIA. Hoy en día no existe en el mercado ningún método validado para la determinación de cortisol en saliva en la especie bovina. Las técnicas de radioinmunoensayo comercializadas para plasma de la especie humana (rango de detección de 10 a 100 ng ml -1 ) no son suficientemente sensibles para el ganado bovino, que presenta concentraciones de cortisol salival muy reducidas (< 4 ng ml -1 ), por ello no es posible su adaptación para esta especie, siendo los métodos de EIA los de elección. El límite de detección de la técnica EIA fue de 0,024 ng ml -1 , mejorando la sensibilidad de técnicas descritas previamente; los coeficientes de variación intra e interensayo fueron de 1,47-7,30% y 2,40-9,78%, respectivamente. La recuperación de cortisol añadido a muestras de saliva fue de 91,36-126,5%. Las pruebas de paralelismo demostraron que es posible la determinación directa de muestras de saliva sin necesidad de extracción previa. La correlación existente entre las...

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“…En regard des concentrations mesurées dans le cadre de cette étude clinique, le procédé de purification (solubilisation, double extraction sur cartouche et en phase liquide) et les moyens de détection semblent plus satisfaisants que ceux décrits lors de la précédente étude (9). Certes, le nombre d'échantillons testés reste faible (n=l 1), mais le fort taux de positivité dans les cheveux (>90 %) atteste de la qualité de la procédure mise en place.…”

Section: Resultsunclassified

“…Les premiers travaux concernant le dosage des corticostéroïdes dans les cheveux ont été récemment publiés (8)(9)(10)(11). Ces substances rejoignent donc la longue liste des molécules incorporées dans les cheveux, mais le faible nombre d'échantillons positifs recensé ne permet pas de préjuger de la sensibilité des techniques mises au point.…”

Section: Introductionunclassified

Identification de la prednisone et de la méthylprednisolone dans les cheveux par HPLC-ES-MS

et al. 2001

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Identification of ten corticosteroids in human hair by liquid chromatography–ionspray mass spectrometry

Cited by 160 publications

References 5 publications

Maternal Influence on Child HPA Axis: A Prospective Study of Cortisol Levels in Hair

Maternal Influence on Child HPA Axis: A Prospective Study of Cortisol Levels in Hair

Validation of an EIA [enzyme immunoassay] technique for the determination of salivary cortisol in cattle

Identification de la prednisone et de la méthylprednisolone dans les cheveux par HPLC-ES-MS

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