Identification of tetracycline antibiotics by electrospray ionization in a quadrupole ion trap

Vartanian, Victor; Goolsby, Brian; Brodbelt, Jennifer S.

doi:10.1016/s1044-0305(98)00078-6

Cited by 77 publications

(66 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As was noted previously, the protonated tetracyclines that fragment by loss of water have structures with a tertiary OH at C-6 [42,43]. Figure 3 shows the effects of collision energy on the lowest energy CID products from [M ϩ H] ϩ ions of oxytetracycline.…”

Section: Positive Ionsmentioning

confidence: 78%

See 1 more Smart Citation

Mass spectral characterization of tetracyclines by electrospray ionization, H/D exchange, and multiple stage mass spectrometry

Kamel

Fouda

Brown

et al. 2002

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Electrospray ionization (ESI) and collisionally induced dissociation (CID) mass spectra were obtained for five tetracyclines and the corresponding compounds in which the labile hydrogens were replaced by deuterium by either gas phase or liquid phase exchange. The number of labile hydrogens, x, could easily be determined from a comparison of ESI spectra obtained with N 2 and with ND 3 as the nebulizer gas. CID mass spectra were obtained forϪ , and produced by ESI using a Sciex API-III plus and a Finnigan LCQ ion trap mass spectrometer. Compositions of product ions and mechanisms of decomposition were determined by comparison of the MS N spectra of the un-deuterated and deuterated species. Protonated tetracyclines dissociate initially by loss of H 2 O (D 2 O) and NH 3 (ND 3 ) if there is a tertiary OH at C-6. The loss of H 2 O (D 2 O) is the lower energy process. Tetracyclines without the tertiary OH at C-6 lose only NH 3 (ND 3 ) initially. MS N experiments showed easily understandable losses of HDO, HN(CH 3 ) 2 , CH 3 Ϫ N¢CH 2 , and CO from fragment ions. The major fragment ions do not come from cleavage reactions of the species protonated at the most basic site. Deprotonated tetracyclines had similar CID spectra, with less fragmentation than those observed for the protonated tetracyclines. The lowest energy decomposition paths for the deprotonated tetracyclines are the competitive loss of NH 3 (ND 3 ) or HNCO (DNCO). Product ions appear to be formed by charge remote decompositions of species de-protonated at the C-10 phenol. (J Am Soc Mass Spectrom 2002, 13, 543-557)

show abstract

Section: Positive Ionsmentioning

confidence: 78%

“…Mass spectrometric techniques for the analysis of tetracycline antibiotics in foods have been reviewed [41]. Some CID spectra of tetracyclines have been reported previously [42,43]. The present study was undertaken to determine structures and mechanisms of formation of principal fragment ions in the ESI mass spectra of five tetracyclines.…”

mentioning

confidence: 99%

Mass spectral characterization of tetracyclines by electrospray ionization, H/D exchange, and multiple stage mass spectrometry

Kamel

Fouda

Brown

et al. 2002

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…Possible structures for these common fragment ions are shown in Fig. S2 (Supporting Information File), but numerous other resonance structures and other isomers could be proposed [33].…”

Section: Lc-ms/ms Methodsmentioning

confidence: 99%

Multi-residue determination of seventeen sulfonamides and five tetracyclines in fish tissue using a multi-stage LC–ESI–MS/MS approach based on advanced mass spectrometric techniques

Dasenaki

Thomaidis

2010

Analytica Chimica Acta

100

View full text Add to dashboard Cite

“…The commonality of these fragments would suggest them as excellent diagnostic ions for the identification of tetracycline analogues in complex sample mixtures. Possible structures for these fragments were proposed recently by Vartanian et al 18 The fragmentation patterns of protonated tetracyclines have been reported previously, 10,18,[28][29][30] and therefore are not presented in detail here.…”

Section: Lc Separation and Electrospray Ms/msmentioning

confidence: 98%

“…Oka et al 10 recently reviewed the current extraction, clean-up, and analysis techniques for the determination of tetracycline contaminants in such matrices. Solid phase extraction with C 18 cartridges is a common choice for sample preparation. 11 However, poor recoveries often necessitate cartridge or sample pre-treatment with ethylenediaminetetraacetic acid (EDTA).…”

mentioning

confidence: 99%

Rapid analysis of tetracycline antibiotics by combined solid phase microextraction/high performance liquid chromatography/mass spectrometry

Lock

Chen

Volmer

1999

Rapid Commun. Mass Spectrom.

View full text Add to dashboard Cite

The technique of solid phase microextraction (SPME) combined on-line with high performance liquid chromatography/mass spectrometry (HPLC/MS) has been applied to the analysis of seven tetracycline analogues. Rapid baseline separation was achieved in under 5 min using a short 3 microm RP-18e cartridge column. Optimisation of the SPME procedure is described including choice of extracting fibre and modification of the sample by heating or salting out of the analytes. Detection limits of 4-40 ng/mL were obtained for the various analogues from extracted aqueous samples and absolute amounts of analyte extracted by the method determined using external calibration. To demonstrate the applicability of the technique for real samples the extraction of tetracycline from milk is described.

show abstract

Identification of tetracycline antibiotics by electrospray ionization in a quadrupole ion trap

Cited by 77 publications

References 33 publications

Mass spectral characterization of tetracyclines by electrospray ionization, H/D exchange, and multiple stage mass spectrometry

Mass spectral characterization of tetracyclines by electrospray ionization, H/D exchange, and multiple stage mass spectrometry

Multi-residue determination of seventeen sulfonamides and five tetracyclines in fish tissue using a multi-stage LC–ESI–MS/MS approach based on advanced mass spectrometric techniques

Rapid analysis of tetracycline antibiotics by combined solid phase microextraction/high performance liquid chromatography/mass spectrometry

Contact Info

Product

Resources

About