Identification of the absorbed components and metabolites in rat plasma after oral administration of Rhizoma Chuanxiong decoction by HPLC–ESI-MS/MS

Zuo, Ai-Hua; Wang, Li; Xiao, Hongbin; Liu, Limin; Liu, Yuhong; Yi, Jiawei

doi:10.1016/j.jpba.2011.08.010

Cited by 71 publications

(64 citation statements)

References 18 publications

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“…In order to obtain as many fragment ions as possible, the peaks detected with intensity over 10,000 were selected for identification. The chemical formulas for all parent and fragment ions of the selected peaks were calculated from the accurate mass using a formula predictor by setting the parameters as follows: C (0-30), H (0-50), O (0-20), S (0-4), N (0-4), Cl (0-4) and ring double bond (RDB) equivalent value [0][1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. Other elements such as P and Br were not considered as they are rarely present in the complex matrix.…”

Section: Data Processingmentioning

confidence: 99%

“…To better understand its mechanism of action and promote its availability as well, the study of its in vivo metabolism behavior is of great importance. In metabolism studies, it is very important to identify and characterize the chemical structure of metabolites [7,8]. Recently, with the development of various data acquisition methods, liquid chromatography-mass spectrometry (LC-MS), especially with high-resolution mass spectrometry (HRMS), has exhibited excellent performance for metabolite detection because of its high-speed and high detection sensitivity [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Metabolites of 6′-Hydroxy-3,4,5,2′,4′-pentamethoxychalcone in Rats by a Combination of Ultra-High-Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry Based on Multiple Data Processing Techniques

et al. 2016

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Abstract:In this study, an efficient strategy was established using ultra-high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) to profile the in vivo metabolic fate of 6 -hydroxy-3,4,5,2 ,4 -pentamethoxychalcone (PTC) in rat urine and feces. The UHPLC-LTQ-Orbitrap method combines the high trapping capacity and MS n scanning function of the linear ion trap along with accurate mass measurements within 5 ppm and a resolving power of up to 30,000 over a wider dynamic range compared to many other mass spectrometers. In order to reduce the potential interferences of endogenous substances, the post-acquisition processing method including high-resolution extracted ion chromatogram (HREIC) and multiple mass defect filters (MMDF) were developed for metabolite detection. As a result, a total of 60 and 35 metabolites were detected in the urine and feces, respectively. The corresponding in vivo reactions such as methylation, hydroxylation, hydrogenation, decarbonylation, demethylation, dehydration, methylation, demethoxylation, sulfate conjugation, glucuronide conjugation, and their composite reactions were all detected in this study. The result on PTC metabolites significantly expanded the understanding of its pharmacological effects, and could be targets for future studies on the important chemical constituents from herbal medicines.

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Section: Data Processingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Metabolites of 6′-Hydroxy-3,4,5,2′,4′-pentamethoxychalcone in Rats by a Combination of Ultra-High-Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry Based on Multiple Data Processing Techniques

et al. 2016

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“…The HPLC-MS/MS analysis provided information on the molecular weights and fragmentation patterns of the compound under investigation. The retention time and MS/MS data of Z-ligustilide were compared with those reported previously [6,[26][27][28].…”

Section: Mspe Of Z-liguistilidementioning

confidence: 99%

Quick and selective extraction ofZ-ligustilide fromAngelica sinensisusing magnetic multiwalled carbon nanotubes

Zeng

Jia

et al. 2015

J. Sep. Science

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A facile and highly efficient magnetic solid-phase extraction method has been developed for Z-ligustilide, the major therapeutic agent in Angelica sinensis. The solid-phase adsorbent material used was prepared by conjugating carbon nanotubes with magnetic Fe3 O4 nanoparticles via a hydrothermal reaction. The magnetic material showed a high affinity toward Z-ligustilide due to the π-π stacking interaction between the carbon nanotubes and Z-ligustilide, allowing a quick and selective exaction of Z-ligustilide from complex sample matrices. Factors influencing the magnetic solid-phase extraction such as the amount of the added adsorbent, adsorption and desorption time, and desorption solvent, were investigated. Due to its high extraction efficiency, this method was proved highly useful for sample cleanup/enrichment in quantitative high-performance liquid chromatography analysis. The proposed method had a linear calibration curve (R(2) = 0.9983) over the concentration between 4 ng/mL and 200 μg/mL Z-ligustilide. The accuracy of the method was determined by the recovery, which was from 92.07 to 104.02%, with the relative standard deviations >4.51%.

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“…It would be detected only up to 1.5 h; because plasma concentrations of LIG were below the limit of detection after 1.5 h. In another pharmacokinetic study of LIG, it was detected in rat brain after 5-20 min of nasal and oral administration, indicating that LIG has a rapid onset of action to enter the central nervous system by permeating blood-brain barrier (Guo et al, 2011). Studies suggested that conjugated with glutathione, cysteine, glucuronic acid and sulphuric acid may be the main metabolic reactions of some phthalides (Zuo et al, 2011). It is well known that pharmacokinetic studies play an increasingly important role in drug discovery and development processes, not only further supporting toxicity or clinical studies but also optimizing drug candidates (Sun et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…To date, there have been some reports on the quantitative analysis of LIG in different plant materials or their extracts by gas chromatography (GC)-flame ionization detection (FID) (Fang et al, 1979;Li et al, 2001), capillary zone electrophoresis (CZE) (Ji et al, 1999), gas chromatography-mass spectrometry (GC-MS) Cho et al, 2007;Deng et al, 2005;Hu & Ding, 2006;Kim et al, 2006a,b;Lao et al, 2004;Li et al, 2006a), liquid chromatography-mass spectrometry (LC-MS) (Lin et al, 1998;Liu et al, 2009;Lu et al, 2004;Qi et al, 2008;Yi et al, 2007;Zschocke et al, 1998), liquid chromatography (LC) (Chao & Chao, 2004;Cui et al, 2006;Hu et al, 2005a,b;Li et al, 2006b;Lu et al, 2005aLu et al, ,b, 2009), bioactivity-guided fractionation method combining countercurrent chromatography (CCC), the MTS cell viability assay and gas chromatography (GC) (Yeh et al, 2012). Although few methods have been reported for the quantification of LIG in biosamples Guo et al, 2009Guo et al, , 2011Li et al, 2009Li et al, , 2011Shi et al, 2006;Yan et al, 2008;Zuo et al, 2011), which are commonly measured as biomarkers. As for extraction of LIG from biological samples, the analytical methods involved multiple steps of liquid-liquid extraction, which used relatively toxic agents such as n-hexaneether, followed by evaporation to prepare and concentrate the sample prior to injection to an LC system, the pretreatment of the biological samples were laborious and time-consuming.…”

Section: Introductionmentioning

confidence: 99%

HPLC method with fluorescence detection for the determination of ligustilide in rat plasma and its pharmacokinetics

Zhang

Qiao

Shi

2013

Pharmaceutical Biology

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Context: Few methods have been reported for the quantification of ligustilide (LIG) in biosamples: the pretreatment of the biological samples were laborious and time-consuming. Objective: A high-performance liquid chromatographic method with fluorescence detection (HPLC-FLD) for the determination of LIG in rat plasma was developed and validated. Pharmacokinetics and bioavailability of LIG were determined by systematic investigation in Sprague-Dawley rats. Materials and methods: LIG was isolated from the volatile oil of Radix Angelica sinensis and further purified by silica gel column chromatography. Podophyllotoxin was used as an internal standard. The analytes were detected by using fluorescence detection at an excitation and emission wavelength of 290 and 395 nm during 0-4 min, and 336 and 453 nm during 4-14 min, respectively. LIG pharmacokinetics was studied in rats after oral and intravenous administration of 12.5, 25 and 50 mg/kg doses. Results: Two calibration curves (Y ¼ 133.49 X À 14.27 (r ¼ 0.9995), Y ¼ 145.61 X þ 13.76 (r ¼ 0.9996)) were constructed in the range of 2.44-10 000 ng/mL for LIG with a lower limit of quantitation of 2.44 ng/mL. Both intra-day and inter-day precision were less than 6%. Accuracy ranged from 88.93 to 99.52%. The recovery ranged from 89.07 to 99.71%. The absolute bioavailability values were 71.36, 68.26 and 75.44% for oral doses of 12.5, 25 and 50 mg/kg, respectively. Conclusion: The present HPLC-FLD method was rapid, sensitive and reliable. LIG was absorbed and eliminated rapidly in rat.

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Identification of the absorbed components and metabolites in rat plasma after oral administration of Rhizoma Chuanxiong decoction by HPLC–ESI-MS/MS

Cited by 71 publications

References 18 publications

Identification of Metabolites of 6′-Hydroxy-3,4,5,2′,4′-pentamethoxychalcone in Rats by a Combination of Ultra-High-Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry Based on Multiple Data Processing Techniques

Identification of Metabolites of 6′-Hydroxy-3,4,5,2′,4′-pentamethoxychalcone in Rats by a Combination of Ultra-High-Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry Based on Multiple Data Processing Techniques

Quick and selective extraction ofZ-ligustilide fromAngelica sinensisusing magnetic multiwalled carbon nanotubes

HPLC method with fluorescence detection for the determination of ligustilide in rat plasma and its pharmacokinetics

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