Identification of the AgNORs, PCNA and ck16 proteins in oral lichen planus lesions

Fonseca, LM da Silva; Carmo, Mav Do

doi:10.1034/j.1601-0825.2001.00747.x

Cited by 34 publications

(6 citation statements)

References 26 publications

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“…PCNA expression were higher in all layers of lichen planus than in both keratosis and normal oral mucosa, it indicates that the keratinocyte proliferation index is higher in lichen planus than in keratosis and normal mucosa. [15] These results were in accordance with our study.…”

Section: Discussionsupporting

confidence: 93%

Immunohistochemical assessment of proliferating cell nuclear antigen protein expression in plaque, reticular and erosive types of oral lichen planus

Pramod

Pandit²,

Desai³

et al. 2014

Ann Med Health Sci Res

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Background:Proliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in the late G1 and S-phase of the cell cycle. Immunodetection of this protein represents a useful marker of the proliferation status of lesions.Aims:The aim of this study is to evaluate the immunohistochemical expression of PCNA in oral lichen planus (OLP) and to assess the PCNA expression in a different layer of epithelium in different types of OLP.Subjects and Methods:A total of 96 cases of histologically proven OLP, 32 cases each of erosive, reticular and plaque type were selected. Two sections were taken from each one for H and E. Other sections were stained according to super sensitive polymer horseradish peroxidase method for identifying PCNA expression.Results:Of the three types of OLP, erosive type showed higher expression of PCNA (average 66.8%, minimum of 55% and maximum of 80.3%) followed by reticular (average 37.7%, minimum of 26% and maximum of 47%) and plaque type (average 17%, minimum of 5% and maximum of 25%) indicating increased proliferative activity. The erosive type also showed higher expression of PCNA in all the layers of epithelium followed by reticular and plaque type.Conclusion:PCNA is a good marker to indicate proliferation status of disease. Out of three types, erosive type possess more proliferative ratio, chances of malignant changes is more in this type.

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Section: Discussionsupporting

confidence: 93%

Immunohistochemical assessment of proliferating cell nuclear antigen protein expression in plaque, reticular and erosive types of oral lichen planus

Pramod

Pandit²,

Desai³

et al. 2014

Ann Med Health Sci Res

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“…An increased PCNA expression secondary to inflammation has been reported previously and hence may have led to such a finding in our study. [ 21 22 ] Moreover, PCNA is characterized by a longer half-life (around 20 h) and results in staining of cells which have recently left the cell cycle. [ 23 ] Deregulated expression of PCNA has also been reported in normal tissues and specimens adjacent to tumors.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cell proliferation in malignant and potentially malignant oral lesions

Madan

Chandra

Raj³

et al. 2015

J Oral Maxillofac Pathol

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Aims:To evaluate the cell proliferation rate by the expression of proliferating cell nuclear antigen (PCNA) and argyrophilic nucleolar organizing region (AgNOR) counts and to assess its usefulness as a marker for malignant potential in oral epithelial lesions.Materials and Methods:The study group included 30 cases of leukoplakia, 15 nondysplastic (NDL), 15 dysplastic (DL), 15 cases of oral squamous cell carcinoma (OSCC) and 5 cases of normal oral mucosa. Formalin fixed paraffin embedded tissues were subjected to immunohistochemical staining for PCNA and AgNOR technique. The PCNA labeling index (LI) and the AgNOR dots were evaluated for the entire sample.Statistical Analysis Used:ANOVA, Tukey honestly significant difference, Pearson's correlation.Results:In this study, the AgNOR count of OSCC was lower than the DL lesions moreover the AgNOR counts were found to be higher in normal mucosa as compared to the DL and the NDL epithelium. The study results also showed that the mean AgNOR count failed to distinguish between DL and NDL lesions. Overall we observed increased PCNA expression from normal epithelium to NDL to DL lesion.Conclusions:Based on the findings of the present study on oral epithelial precancerous and cancerous lesions we conclude that mean AgNOR count alone cannot be a valuable parameter to distinguish between the normal, NDL, DL epithelium and OSCC but, on the other hand, we found out that PCNA can be a useful biomarker for delineating normal epithelium from DL epithelium and OSCC.

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“…Previous studies[234567891011121314151617181920] included most but not all the parameters as in the present study, providing evidence of variation in anti-VEGF, anti-CD34 and anti-PCNA antibody staining. However, few studies[2345671215181920] have not shown statistically significant results among the parameters considered.…”

Section: Discussionmentioning

confidence: 90%

“…An overall increase in mean scores from normal to OLP, OL, OSF and different grades of OSCC was similar to other studies. [234567891011121314151617181920]…”

Section: Discussionmentioning

confidence: 99%

Role of cell proliferation and vascularity in malignant transformation of potentially malignant disorders

Sheelam¹,

Reddy

Kulkarni

et al. 2018

J Oral Maxillofac Pathol

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Background:Significant increase in cell proliferation and vascularity occurs during the transition from normal oral mucosa through differing degrees of dysplasia to oral squamous cell carcinoma (OSCC).Aims:To evaluate the cell proliferation and vascularity in potentially malignant disorders and OSCC.Settings and Design:Proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF) and CD34 were quantified immunohistochemically (IHC) using anti-PCNA, anti-VEGF and anti-CD34 antibody.Materials and Methods:A total of 60 archival specimens included 10 oral lichen planus, 10 oral leukoplakia, 10 oral submucous fibrosis and 30 OSCC (well differentiated, moderately differentiated and poorly differentiated), and also, 10 normal oral mucosa as control group were taken. PCNA, VEGF and CD34 expression was assessed in relation to the localization and area of IHC-stained cells.Statistical Analysis:One-way analysis of variance test and post hoc least significant difference test were assessed for statistical significance.Results:Cell proliferation and vascularity appeared to increase gradually with disease progression.Conclusion:Upregulation of cell proliferation and vascularity indicates their possible role in malignant transformation of potentially malignant disorders.

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Identification of the AgNORs, PCNA and ck16 proteins in oral lichen planus lesions

Cited by 34 publications

References 26 publications

Immunohistochemical assessment of proliferating cell nuclear antigen protein expression in plaque, reticular and erosive types of oral lichen planus

Immunohistochemical assessment of proliferating cell nuclear antigen protein expression in plaque, reticular and erosive types of oral lichen planus

Evaluation of cell proliferation in malignant and potentially malignant oral lesions

Role of cell proliferation and vascularity in malignant transformation of potentially malignant disorders

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