Identification of the Auxin-responsive Element, AuxRE, in the Primary indoleacetic Acid-inducible Gene, PS-IAA4/5, of Pea (Pisum sativum)

Ballas, Nurit; Wong, Lu-Min; Theologis, Athanasios

doi:10.1006/jmbi.1993.1537

Cited by 185 publications

(136 citation statements)

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“…Samsun) have been generated expressing either PS-IAA4/5-GUS or PS-IAA6-GUS. Previous studies indicated preferential expression of PS-IAA4/5 and PS-IAA6 in elongating pea epicotyl (Ballas et al, 1993;Theologis et aL, 1985). Therefore, we focused our histochemical analysis on patterns of spatio-temporal GUS expression in early development of etiolated and light-grown seedlings.…”

Section: Analysis Of Ps-iaa4/5-gus and Ps-iaa6-gus Expression In Tranmentioning

confidence: 99%

“…Tobacco (Nicotiana tabacum cv. Samsun) was stably transformed using the leaf disc method as previously described (Ballas et al, 1993). Fifteen independent transformants transgenic for PS-/AA4/5-GUS and 30 independent lines carrying the PS-IAA6-GUStransgene have been generated.…”

Section: Plant Transformationmentioning

confidence: 99%

“…As for PS-IAA4/5 and PS-IAA6, little is known of their spatial and temporal activities in developing plants. Previous studies have shown that both genes are preferentially expressed in the elongation zone of etiolated pea seedlings (Ballas et aL, 1993;Theologis et al, 1985). Here, we present a detailed histochemical characterization of PS-IAA4/5 and PS-IAA6 expression during early plant development, using transgenic tobacco seedlings with promoter-~-glucuronidase (GUS) gene fusions.…”

Section: Introductionmentioning

confidence: 96%

“…The induction of both genes by IAA qualifies as a primary response (Koshiba et al, 1995;Theologis et al, 1985). P$-IAA4/5 and PS-/AA6 have been structurally characterized (Oeller et al, 1993), and the auxin-responsive domains of PS-IAA4/5 have been identified (Ballas et al, 1993. Both genes are members of a multigene family in pea, and the proteins encoded are related to products of early auxin-inducible genes from various plant species Oeller and Theologis, 1995;Oeller et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Differential activation of the primary auxin response genes, PS‐IAA4/5 and PS‐IAA6, during early plant development

et al. 1996

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SummaryThe plant growth hormone auxin typified by indoleacetic acid (IAA) transcriptionally activates early genes in pea, PS.IAA4/5 and PS-IAA6, that are members of a multigene family encoding short-lived nuclear proteins. To gain first insight into the biological role of PS-IAA4/5 and PS-IAA6, promoter-~-glucuronidase (GUS) gene fusions were constructed and their expression during early development of transgenic tobacco seedlings was examined. The comparative analysis reveals spatial and temporal expression patterns of both genes that correlate with cells, tissues, and developmental processes known to be affected by auxin. GUS activity in seedlings of both transgenic lines is located in the root meristem, sites of lateral root initiation and.in hypocotyls undergoing rapid elongation. In addition, mutually exclusive cell-specific expression is evident. For instance, PS-IAA4/5-GUS but not PS-IAA6-GUS is expressed in root vascular tissue and in guard cells, whereas only PS-IAA6.-GUS activity is detectable in glandular trichomes and redistributes to the elongating side of the hypocotyl upon gravitropic stimulation. Expression of PS-IAA4/5 and PS-IAA6 in elongating, dividing, and differentiating cell types indicates multiple functions during development. The common and yet distinct activity patterns of both genes suggest a combinatorial code of spatio-temporal co-expression of the various PS-IAA4/ 5-like gene family members in plant development that may mediate cell-specific responses to auxin.

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Section: Analysis Of Ps-iaa4/5-gus and Ps-iaa6-gus Expression In Tranmentioning

confidence: 99%

Section: Plant Transformationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Differential activation of the primary auxin response genes, PS‐IAA4/5 and PS‐IAA6, during early plant development

et al. 1996

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“…The second is the subsequent induction of a certain subset of genes, curiously including those that encode AUX/IAA proteins (Hagen and Guilfoyle, 1985;Theologis et al, 1985). A large part of the transcriptional response is believed to be mediated by the binding of auxin response factors (ARFs; Ulmasov et al, 1997;Guilfoyle et al, 1998) to auxin response elements (Ballas et al, 1993;Ulmasov et al, 1995) found upstream of auxin-inducible genes. In the basal state, AUX/IAA proteins sequester ARFs by heterodimerizing with them and hence prevent ARFs from homodimerizing and activating auxin-inducible genes (Ulmasov et al, 1999;Worley et al, 2000;Reed, 2001;Rogg and Bartel, 2001).…”

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confidence: 99%

AtCAND1, A HEAT-Repeat Protein That Participates in Auxin Signaling in Arabidopsis

2004

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Auxin affects many aspects of plant growth and development. We previously used chemical genetics to dissect auxin-signaling mechanisms and identified a small molecule, sirtinol, that constitutively activated auxin signaling (Y. Zhao et al. [2003], Science 301: 1107–1110). Here we describe the isolation, characterization, and cloning of an Arabidopsis mutant Atcand1-1 that emerged from a genetic screen for mutants insensitive to sirtinol. Loss-of-function mutants of AtCAND1 were resistant to sirtinol and auxin, but not to gibberellins or brassinolide. Atcand1 displayed developmental phenotypes similar to those of axr1, namely, short petioles, downwardly curling leaves, short inflorescence, and reduced fertility. AtCAND1 is homologous to human CAND1, a protein that is composed almost entirely of HEAT-repeat units and has been implicated in regulating the assembly and disassembly of the SCF protein degradation machinery. Taken together with previous biochemical studies, this work helps to elucidate the roles of AtCAND1 in protein degradation and auxin signaling.

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Herbicide detoxification by glutathioneS-transferases as implicated from X-ray structures

et al. 1999

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Herbicide selectivity is a major factor in agricultural weed control and results from the differential detoxification ability of plant species. The special agronomic value of plant GSTs originates mainly from their metabolic herbicide detoxification properties that enhance the herbicide tolerance of crops. Glutathione S‐transferases (GSTs) are a ubiquitous family of multifunctional enzymes involved in the metabolization of a broad variety of xenobiotics (eg herbicides in plants) and reactive endogenous compounds through covalent linkage to glutathione. The metabolization of FOE 5043 results in a GSH conjugate that is subsequently degraded by release of the thiadiazole moiety. The comparison of crystal structures of different GST classes, including plant GSTs, provides a model system to understand active‐site interactions on a molecular level. Additional protein structures of three plant GSTs (Arabidopsis thaliana GST, GST I and III from Zea mays var. mutin) in complex with several ligands (S‐hexylglutathione, S‐lactoylglutathione, and FOE 5043) may be tools to supply detailed knowledge for the rational design of new herbicides and GSTs for selectivity optimization in crops. © 1999 Society of Chemical Industry

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Identification of the Auxin-responsive Element, AuxRE, in the Primary indoleacetic Acid-inducible Gene, PS-IAA4/5, of Pea (Pisum sativum)

Cited by 185 publications

References 0 publications

Differential activation of the primary auxin response genes, PS‐IAA4/5 and PS‐IAA6, during early plant development

Differential activation of the primary auxin response genes, PS‐IAA4/5 and PS‐IAA6, during early plant development

AtCAND1, A HEAT-Repeat Protein That Participates in Auxin Signaling in Arabidopsis

Herbicide detoxification by glutathioneS-transferases as implicated from X-ray structures

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