Identification of the B, Q, and native Brazilian biotypes of the Bemisia tabaci species complex using Scar markers

Queiroz, P. R.; Martins, E. S.; Klautau, Nazaré; Lima, L. H. C.; Praça, L. B.; Monnerat, R. G.

doi:10.1590/s0100-204x2016000500016

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…In our study, we find that SCAR markers such as BaA and CWF-1 (AY841800, DQ174543) are linked to Q-BtabCSP2 gene, while B-BtabCSP2 only has OPT12 SCAR marker (EU660887). Such a specificity observed in SCAR sequence motifs linked to CSP is likely to help develop a new way of genotyping, particularly in the sweetpotato whitefly B. tabaci ( Figure 1 C) [ 15 , 37 ]. BaA, CWF-1 and OPT12 should be investigated in detail, particularly because such specific SCARs seem to associate with insecticide resistance not only in whiteflies, but also in other insect species such as the parasitoid jewel wasp Nasonia vitripennis [ 15 , 38 ].…”

Section: Intron Variation and Retrotransposition In A Conserved Gementioning

confidence: 99%

Genotyping and Bio-Sensing Chemosensory Proteins in Insects

Liu

Arnaúd

Offmann

et al. 2017

Sensors

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Genotyping is the process of determining differences in the genetic make-up of an individual and comparing it to that of another individual. Focus on the family of chemosensory proteins (CSPs) in insects reveals differences at the genomic level across various strains and biotypes, but none at the level of individuals, which could be extremely useful in the biotyping of insect pest species necessary for the agricultural, medical and veterinary industries. Proposed methods of genotyping CSPs include not only restriction enzymatic cleavage and amplification of cleaved polymorphic sequences, but also detection of retroposons in some specific regions of the insect chromosome. Design of biosensors using CSPs addresses tissue-specific RNA mutations in a particular subtype of the protein, which could be used as a marker of specific physiological conditions. Additionally, we refer to the binding properties of CSP proteins tuned to lipids and xenobiotic insecticides for the development of a new generation of biosensor chips, monitoring lipid blood concentration and chemical environmental pollution.

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Section: Intron Variation and Retrotransposition In A Conserved Gementioning

confidence: 99%

Genotyping and Bio-Sensing Chemosensory Proteins in Insects

Liu

Arnaúd

Offmann

et al. 2017

Sensors

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“…Whitefly, Bemisia tabaci (Gennadius, 1889), is a globally significant pest causing economic losses in various important crops, including tomatoes (Zaidi et al, 2017). The most aggressive form of whitefly, known as biotype B, Bemisia argentifolii (Bellows & Perring, 1994), is also the most common and abundant (Queiroz et al, 2016). It has a high capacity for transmitting geminiviruses that cause phytotoxic anomalies, also induced by toxins injected during insect feeding (Bragard et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Resistance to whitefly mediated by the Mi gene, acylsugars, and zingiberene in tomato

Oliveira,

Maluf,

Andrade

et al. 2023

Com. Sci.

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This study aimed to quantify and compare the effects of Mi gene, high leaf levels of acylsugars (AA), and zingiberene (ZGB) individually and in combination, regarding resistance of tomato lines to Bemisia argentifolii. The experiment included 21 genotypes selected simultaneously for high AA and ZGB content, cloned through rooting of axillary shoots with homogeneous sizes, and seven control lines produced from seeds, including 2 genotypes rich in AA (TOM-687 and TOM-688), two genotypes rich in ZGB (TOM-703 and TOM-704), and three lines with low levels of allelochemicals (cv. Santa Clara, TOM-584, and TOM-684), where the TOM-684 line carried the Mi gene. The experiment was conducted in a greenhouse using a randomized complete block design, comprising 28 treatments with 4 replications and 1 plant per plot. The AA and ZGB-rich lines showed higher resistance compared to the other control lines with low levels of allelochemicals, but the TOM-684 control line carrying the Mi gene was more resistant than the non-carriers ('Santa Clara' and TOM-584). The level of resistance conferred by ZGB was similar to that presented by AA. Both ZGB and AA provided higher levels of resistance compared to the Mi gene. The clones selected for both ZGB and AA simultaneously exhibited lower numbers of eggs and nymphs compared to the susceptible controls and were more resistant than the TOM-684 treatment. Overall, the clones even outperformed the lines rich in only one of the allelochemicals, demonstrating a synergistic effect between AA and ZGB for resistance when combined in the same tomato line.

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“…Nowadays, the more reproducible and informative method available to determine the genetic affiliation of a B. tabaci individual is based on molecular data, using markers such as Random Amplified Polymorphic DNA (RAPD) and Internal Transcribed Sequence (ITS) (Rabello et al, 2008), Sequence Characterized from Amplified Regions (SCAR) (Queiroz et al, 2016) and mitochondrial genes such as cytochrome oxidase 1 (De Barro, 2005;Marubayashi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Description of the molecular profiles of Bemisia tabaci (Hemiptera: Aleyrodidae) in different crops and locations in Brazil

Queiroz¹,

Lima²,

Martins³

et al. 2017

J. Entomol. Nematol.

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The Whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) causes damages to crops by feeding and/or transmitting various plant pathogenic viruses. Morphologically indistinguishable populations of B. tabaci can exhibit variable molecular features and biological behavior. The objective of this work was to present the molecular profile and genetic variability of B. tabaci collected from several crops and localities in Brazil. The molecular identification of 100 whiteflies samples collected from these crops and localities in Brazil revealed 14% of non B biotype of B. tabaci. About the biotypes of B. tabaci, 2% indigenous Brazilian whitefly was found, 1% A biotype, 2% Q biotype, 1% African cassava and 80% B biotype. The dendrogram obtained by molecular markers showed that biotype A of B. tabaci from the United States differed from the other biotypes as it showed around 8% of genetic similarity. For the remaining populations previously identified as biotype B, separation was observed among them based on location and crop of collection. The B. tabaci native Brazilian biotype did not form a separate grouping from the B biotype. This information may serve as a basis for studies aiming to identify which factors influence the population distribution in a given area.

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Identification of the B, Q, and native Brazilian biotypes of the Bemisia tabaci species complex using Scar markers

Cited by 6 publications

References 17 publications

Genotyping and Bio-Sensing Chemosensory Proteins in Insects

Genotyping and Bio-Sensing Chemosensory Proteins in Insects

Resistance to whitefly mediated by the Mi gene, acylsugars, and zingiberene in tomato

Description of the molecular profiles of Bemisia tabaci (Hemiptera: Aleyrodidae) in different crops and locations in Brazil

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