Identification of the bZIP gene family and regulation of metabolites under salt stress in isatis indigotica

Abstract

The bZIP transcription factor family plays important roles in plant growth and development, response to stress, and regulation of secondary metabolite biosynthesis. The identification and molecular function of bZIP gene have been deeply studied in the model plant Arabidopsis thaliana, but it has not been reported in the medicinal plant Isatis indigotica. In this study, 65 IibZIP genes were identified in the genome of I. indigotica, which were distributed on seven chromosomes, were highly conserved, could be classified into 11 subgroups. Transcriptomic and metabolomic data for leaves of I. indigotica exposed to salt stress were analyzed to construct an IibZIP gene co-expression network and metabolite correlation network. Seventeen IibZIP genes were co-expressed with 79 transcription factors, and GO and KEGG enrichment analysis showed that most of these genes were associated with abiotic stress and hormone responses of plants. 17 IibZIP genes regulated 110 metabolites through 92 transcription factor associations. In addition, IibZIP23, IibZIP38 and IibZIP51 were associated with six metabolites including three alkaloids (quinoline alkaloid stylopine, indole alkaloids tabersonine and indole-3-acetic acid), flavonoid myricetin 3-O-galactoside, and two primary metabolites 2-hydroxy-6-aminopurine, 3-dehydroshikimic acid were strongly correlated. This study provides data for identification of the IibZIP gene family and their regulation of metabolites in response to salt stress.

Systematic evolution of bZIP transcription factors in Malvales and functional exploration of AsbZIP14 and AsbZIP41 in Aquilaria sinensis

“…The bZIP family of TFs is widely distributed in the plant kingdom and their subfamilies are analyzed in detail in various plants, especially the staple oil or food crops (wheat, rice, maize, Olive, soybean), the model plant ( Arabidopsis thaliana ) and in horticultural crops (litchi, pear, walnut, pomegranate, Chinese jujube, and sweet potato) ( Wei et al., 2012 ; Zhang et al., 2014 ; Dröge-Laser et al., 2018 ; Zhang et al., 2018a ; Agarwal et al., 2019 ; Yang et al., 2019 ; Rong et al., 2020 ; Zhang et al., 2020b ; Ma et al., 2021 ; Hou et al., 2022 ; Wang et al., 2022c ; Zhang et al., 2022b ). Moreover, the bZIP gene families in medicinal plants such as Carthamus tinctorius , Cannabis sativa , Andrographis paniculata , Isatis indigotica , and Salvia miltiorrhiza were studied in recent years ( Zhang et al., 2018b ; Li et al., 2020 ; Guan et al., 2022 ; Jiang et al., 2022 ; Lu et al., 2022 ). Interestingly, bZIPs were also involved in mediated or increased the accumulation of effective ingredients in medicinal plants ( Hao et al., 2019 ).…”

Identification and Expression Profiling of the bZIP Gene Family Throughout the Genome in Phoebe bournei under Abiotic Stress

“…This gene regulates the synthesis of avonoids, as well as light and drought tolerance in plants. It is feasible that the MYB gene and the PbbZIP protein interact to create a regulatory network (Mitra 2018;Jiang et al 2022).…”

Systematic Identification and Functional Analysis of the Hypericum perforatum L. bZIP Gene Family Indicating That Overexpressed HpbZIP69 Enhances Drought Resistance

“…The H. perforatum bZIP gene family is a multigene family that is widely present and highly conserved in eukaryotes, where it plays a vital role in regulating plants’ growth and development, participating in stress response, and promoting the synthesis of secondary metabolites [ 32 ]. In this study, based on a genome-wide database of H. perforatum , we screened 79 HpbZIP genes and the relationships between their physical and chemical properties, system development evolution, gene structures, conserved motifs, gene function enrichment regions, and cis-acting elements, as well as under different tissues and different hormone gene expression patterns, and we conducted a preliminary analysis.…”