Identification of the Cellobiose 2-Epimerase Gene in the Genome of<i>Bacteroides fragilis</i>NCTC 9343

Senoura, Takeshi; Taguchi, Hidenori; Ito, Shigeaki; Hamada, Shigeki; Matsui, Hirokazu; Fukiya, Satoru; Yokota, Atsushi; Watanabe, Jun; Wasaki, Jun; Ito, Susumu

doi:10.1271/bbb.80691

Cited by 57 publications

(39 citation statements)

References 27 publications

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“…However, the amino acid sequence of EpiA shows a high sequence identity (32-42%) to those of several other reported CEs. [9][10][11][12][13][14][15][16] UnkA, whose function currently remains unknown, has been classified into glycoside hydrolase family 130 based on its amino acid sequence. 17) Glycoside hydrolase family 130 contains MGP, β-1,4-mannooligosaccharide phosphorylase, and β-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase (EC 2.4.1.320).…”

Section: Functional Reassignment Of Cellvibrio Vulgarismentioning

confidence: 99%

Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-β-d-mannosyl-d-glucose phosphorylase-like protein, UnkA

Saburi

Tanaka

Muto

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Section: Functional Reassignment Of Cellvibrio Vulgarismentioning

confidence: 99%

Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-β-d-mannosyl-d-glucose phosphorylase-like protein, UnkA

Saburi

Tanaka

Muto

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…11,14) We also reported the gene sequences and catalytic properties of CEs from Eubacterium cellulosolvens 15) and Bacteroides fragilis cells. 16 Fig. 3).…”

Section: Cellobiose 2-epimerase (Ce)mentioning

confidence: 98%

“…Cellobitol, lactitol, N acetyl D glucosamine, N acetyl D mannosamine and N acetyl lactosamine did not serve as substrates. At present, functional CEs have been reported to occur only in bacteria, 11,13,15,16) and their physiological role(s) has not been clarified yet. They share less than 20% identity to aAGE, 7) and no homology was observed with the other heterogonous epimerases.…”

Section: Cellobiose 2-epimerase (Ce)mentioning

confidence: 99%

“…However, the phylogenetic position of CmEpiA is on a branch different from those of CEs. 16) CE can 2 epimerize β mannobiose but has no activity toward mannose. 18) Recent study with the RmlC from Pseudomonas aeruginosa (PDB code 2ixi); clarifies that the enzyme has a double stranded β helix structure, as shown in Fig.…”

Section: Mannose 2-epimerase Activitymentioning

confidence: 99%

“…For instance, functional CE and CE like proteins have been suggested to distribute widely in the microbial genomes of various genera in the phyla Bacteroidetes, Firmicutes and Proteobacteria. 16) Unidentified and useful carbohydrate epimerases would therefore be found in microbial genomes that have not yet been sequenced. Metagenomic approaches will also allow us to discover diverse carbohydrate epimerases.…”

Section: Prospects For Future Progressmentioning

confidence: 99%

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Catalysis, Structures, and Applications of Carbohydrate Epimerases

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Production and Bioactivity of Oligosaccharides Derived from Lactose

Villamiel

Montilla

Olano

et al. 2014

Food Oligosaccharides

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In an attempt to offer a broad overview on lactose applications as a source of bioactive carbohydrates, in this chapter the authors have collected the most recent investigations on methods to produce these compounds, which have a positive effect on gut microbiota. Chemical and biological productions of tagatose, lactulose, lactosucrose, lactulosucrose and galactooligosaccharides, among others, have been described. Moreover, their main uses, based on their bioactivity and techno-functional properties, are also shown together with a brief description of purification methods of the mixtures obtained. Particular attention has been paid to a new generation of oligosaccharides derived from lactulose with a potential prebiotic effect, which is even better than that of this recognised functional ingredient. These novel oligosaccharides, mainly composed of 6'-galactosyl lactulose and 1-galactosyl lactulose can be obtained with good yields either by transgalactosylation of lactulose or by chemical isomerisation under basic media of galactooligosaccharides; the latter constitutes a good and economic option.

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Identification of the Cellobiose 2-Epimerase Gene in the Genome ofBacteroides fragilisNCTC 9343

Cited by 57 publications

References 27 publications

Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-β-d-mannosyl-d-glucose phosphorylase-like protein, UnkA

Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-β-d-mannosyl-d-glucose phosphorylase-like protein, UnkA

Catalysis, Structures, and Applications of Carbohydrate Epimerases

Production and Bioactivity of Oligosaccharides Derived from Lactose

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