Identification of the gene for the monomeric alkaline phosphatase of Vibrio cholerae serogroup O1 strain

Majumdar, Anasuya; Ghatak, Atreyi; Ghosh, Ram Krishna

doi:10.1016/j.gene.2004.11.005

Cited by 28 publications

(32 citation statements)

References 23 publications

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“…Chromosomal DNA from V. cholerae strain was prepared from overnight cultures according to previously described method (Majumder et al, 2005). Restriction endonucleases and T4 DNA ligase were obtained from New England Biolab.…”

Section: Dna Preparations and Manipulationsmentioning

confidence: 99%

“…Conjugation was conducted between recipient streptomycin resistant V. cholerae strain (PL91) and donor E. coli SM10: λ pir transformed with pSVM as well as pSC (Waldor and Mekalanos, 1996;Majumder et al, 2005). luxO and luxU mutants of PL 91 were screened by streaking onto LA plates containing ampicillin (100 μg ml − 1 ) and streptomycin (10 μg ml − 1 ).…”

Section: Bacterial Conjugationmentioning

confidence: 99%

“…PCR products carrying luxO and luxU genes were labeled with [α 32 P] dATP by random priming kit (New England Biolabs) and were used as probes. Southern blot experiments were performed under stringent conditions (Majumder et al, 2005).…”

Section: Dna Hybridizationmentioning

confidence: 99%

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Identification of a constitutively active variant of LuxO that affects production of HA/protease and biofilm development in a non-O1, non-O139 Vibrio cholerae O110

Raychaudhuri

Jain

Dongre

2006

Gene

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Section: Dna Preparations and Manipulationsmentioning

confidence: 99%

Section: Bacterial Conjugationmentioning

confidence: 99%

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Identification of a constitutively active variant of LuxO that affects production of HA/protease and biofilm development in a non-O1, non-O139 Vibrio cholerae O110

Raychaudhuri

Jain

Dongre

2006

Gene

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“…Recently, a novel phosphatase gene (phoX) was identified in Vibrio cholerae (Majumdar et al, 2005). Although still poorly characterized, PhoX is an alkaline phosphatase and a member of the Pho regulon (von Kruger et al, 2006), but shares no homology to PhoA.…”

Section: Introductionmentioning

confidence: 99%

“…Although still poorly characterized, PhoX is an alkaline phosphatase and a member of the Pho regulon (von Kruger et al, 2006), but shares no homology to PhoA. In contrast to PhoA, which is a homodimer activated by Zn 2 þ and Mg 2 þ , PhoX is a monomeric enzyme activated by Ca 2 þ (Roy et al, 1982;Majumdar et al, 2005). Furthermore, PhoA is secreted to the periplasm or the extracellular space through the Sec pathway (Kim and Wyckoff, 1991), whereas PhoX is exported through the twin arginine transport system, which is able to translocate fully folded proteins across membranes (Monds et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The alkaline phosphatase PhoX is more widely distributed in marine bacteria than the classical PhoA

2009

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Phosphorus (P) is a vital nutrient for all living organisms and may control the growth of bacteria in the ocean. Bacteria induce alkaline phosphatases when inorganic phosphate (Pi) is insufficient to meet their P-requirements, and therefore bulk alkaline phosphatase activity measurements have been used to assess the P-status of microbial assemblages. In this study, the molecular basis of marine bacterial phosphatases and their potential role in the environment were investigated. We found that only a limited number of homologs to the classical Escherichia coli alkaline phosphatase (PhoA) were present in marine isolates in the Bacteroidetes and γ-proteobacteria lineages. In contrast, PhoX, a recently described phosphatase, was widely distributed among diverse bacterial taxa, including Cyanobacteria, and frequently found in the marine metagenomic Global Ocean Survey database. These taxa included ecologically important groups such as Roseobacter and Trichodesmium. PhoX was induced solely upon P-starvation and accounted for approximately 90% of the phosphatase activity in the model marine bacterium Silicibacter pomeroyi. Analysis of the available transcriptomic datasets and their corresponding metagenomes indicated that PhoX is more abundant than PhoA in oligotrophic marine environments such as the North Pacific Subtropical Gyre. Those analyses also revealed that PhoA may be important when Bacteroidetes are abundant, such as in algal bloom episodes. However, PhoX appears to be much more widespread. Its identification as a gene that mediates organic P acquisition in ecologically important groups, and as a marker of Pi-stress, constitutes an important step toward a better understanding of the marine P cycle.

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The phosphate‐starvation response in Vibrio cholerae O1 and phoB mutant under proteomic analysis: Disclosing functions involved in adaptation, survival and virulence

et al. 2006

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A proteomic analysis of a wild-type and of a phoB mutant showed that Vibrio cholerae expresses genes of two major regulons in response to phosphate starvation. The Pho regulon, expressed by the wild-type, allowed the cells to adapt to the new environment. Induction of the general stress regulon was mainly observed in the phoB mutant as a strategy to resist stress and survive. Some functions of the adaptative and survival responses play roles in the pathogenicity of the bacteria. Among the members of the Pho regulon, we found a porin described as an important factor for the intestinal colonisation. Other functions not obviously related to phosphate metabolism, expressed preferentially by the wild-type cells, have also been implicated in virulence. These findings might explain the lack of virulence of the phoB mutant. The Pho regulon picture of V. cholerae, however, will not be complete until minor members and membrane proteins are identified. Among the phosphate-starvation induced genes we have found 13 hypothetical ones and for some of them functions have been assigned. The majority of the genes identified here have not been described before, thus they could be used to expand the proteomic reference map of V. cholerae El Tor.

show abstract

Identification of the gene for the monomeric alkaline phosphatase of Vibrio cholerae serogroup O1 strain

Cited by 28 publications

References 23 publications

Identification of a constitutively active variant of LuxO that affects production of HA/protease and biofilm development in a non-O1, non-O139 Vibrio cholerae O110

Identification of a constitutively active variant of LuxO that affects production of HA/protease and biofilm development in a non-O1, non-O139 Vibrio cholerae O110

The alkaline phosphatase PhoX is more widely distributed in marine bacteria than the classical PhoA

The phosphate‐starvation response in Vibrio cholerae O1 and phoB mutant under proteomic analysis: Disclosing functions involved in adaptation, survival and virulence

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