Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Li, Shaobin; Zhou, Huitong; Gong, Hua; Zhao, Fangfang; Wang, Jiqing; Liu, Xiu; Luo, Yuzhu; Hickford, Jonathan G. H.

doi:10.3390/genes8010027

Cited by 44 publications

(50 citation statements)

References 19 publications

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“…Keratin-associated protein 15-1 gene is clustered with HGT-KAP genes and some HS-KAP genes on sheep chromosome 1, including KRTAP6-1, KRTAP6-3, KRTAP20-2, KRTAP22-1 and KRTAP26-1, for which effects on wool traits have been described. The effects described here for KRTAP15-1 are similar to those reported for KRTAP22-1 (Li et al, 2017a), but different to those for KRTAP6-1, KRTAP6-3, KRTAP20-2 and KRTAP26-1, where the effect is observed with either fibre diameterassociated traits (Zhou et al, 2015;Li et al, 2017bLi et al, , 2017c or MFC (Bai et al, 2018). Given that KRTAP15-1 is positioned closer to KRTAP26-1 and KRTAP6-3, than to KRTAP22-1, it is therefore suggested that the effect described here for KRTAP15-1 is not due to its proximity to KRTAP22-1 but is instead the result of its independent action or linkage to variation closely proximal to the gene in the upstream or downstream regions.…”

Section: Discussionsupporting

confidence: 85%

Variation in the ovine keratin-associated protein 15-1 gene affects wool yield

Gong

Zhou

et al. 2018

J. Agric. Sci.

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Keratin-associated proteins (KAPs) are constituents of wool and hair fibres and are believed to play an important role in determining the characteristics of the fibres. In the current study, a polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) approach was used to screen for variation in the ovine KAP15-1 gene (KRTAP15-1). Four PCR-SSCP banding patterns, representing four different variants (named A to D), were detected. Four single nucleotide polymorphisms were found within the coding region and three of these were non-synonymous. The effect of this genetic variation on wool traits was investigated in 396 Merino × Southdown-cross sheep. Of the three variants found in these sheep (A, B and C), the presence of B was found to be associated with decreased wool yield, while C was associated with increased wool yield and decreased fibre diameter standard deviation. Sheep of genotype AC had a higher wool yield than those of genotype AA or AB.

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Section: Discussionsupporting

confidence: 85%

Variation in the ovine keratin-associated protein 15-1 gene affects wool yield

Gong

Zhou

et al. 2018

J. Agric. Sci.

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“…Compared to humans and sheep, the number of KAP genes (KRTAPs) identified to date in the goat genome is relatively small. For example, at least 80 functional KRTAPs from 25 families and 36 KRTAPs from 19 families have been reported in humans [3,4] and sheep [2,[5][6][7][8][9][10] respectively. However, only 14 KRTAPs from 10 families have been identified in goats [11], with this suggesting that many of the KAP genes found in humans and sheep, remain to be identified.…”

Section: Introductionmentioning

confidence: 99%

Identification of Caprine KRTAP28-1 and Its Effect on Cashmere Fiber Diameter

Wang

Zhou

Hickford

et al. 2020

Genes

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The keratin-associated proteins (KAPs) are constituents of cashmere fibers and variation in many KAP genes (KRTAPs) has been found to be associated with fiber traits. The gene encoding the high-sulphur KAP28-1 has been described in sheep, but it has not been identified in the goat genome. In this study, a 255-bp open reading frame on goat chromosome 1 was identified using a search of similar sequence to ovine KRTAP28-1, and that would if transcribed and translated encode a high sulphur KAP. Based on the analysis of polymerase chain reaction amplicons for the goat nucleotide sequences in 385 Longdong cashmere goats in China, five unique banding patterns were detected using single strand conformation polymorphism analysis. These represented five DNA sequences (named variants A to E) and they had the highest resemblance to KRTAP28-1 sequences from sheep, suggesting A–E are variants of caprine KRTAP28-1. DNA sequencing revealed a 2 or 4-bp deletion and eleven nucleotide sequence differences, including four non-synonymous substitutions. Of the four common variants (A, B, C and D) found in these goats, the presence of variant A was associated with decreased mean fiber diameter and this effect appeared to be additive. These results indicate that caprine KRTAP28-1 variation might have value as a molecular marker for reducing cashmere mean fiber diameter.

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“…Two SNPs in keratin-associated protein 22.1 ( KAP 22 . 1 ) gene and six SNPs in desmoglein 4 ( DSG 4 ) gene were shown to influence wool crimp in Southdown × Merino cross lambs and Tan sheep, respectively [4, 5]. In addition, our previous study showed that nine SNPs of methionine synthase ( MTR ) gene were associated with average wool fiber diameter in Chinese Merino sheep [6].…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep

Chu

Zhang

et al. 2017

PLoS ONE

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Follistatin (FST) is involved in hair follicle morphogenesis. However, its effects on hair traits are not clear. This study was designed to investigate the effects of FST gene single nucleotide polymorphisms (SNP) on wool quality traits in Chinese Merino sheep (Junken Type). We performed gene expression analysis, SNP detection, and association analysis of FST gene with sheep wool quality traits. The real-time RT-PCR analysis showed that FST gene was differentially expressed in adult skin between Chinese Merino sheep (Junken Type) and Suffolk sheep. Immunostaining showed that FST was localized in inner root sheath (IRS) and matrix of hair follicle (HF) in both SF and Suffolk sheep. Sequencing analysis identified a total of seven SNPs (termed SNPs 1–7) in the FST gene in Chinese Merino sheep (Junken Type). Association analysis showed that SNP2 (Chr 16. 25,633,662 G>A) was significantly associated with average wool fiber diameter, wool fineness SD, and wool crimp (P < 0.05). SNP4 (Chr 16. 25,633,569 C>T) was significantly associated with wool fineness SD and CV of fiber diameter (P < 0.05). Similarly, the haplotypes derived from these seven identified SNPs were also significantly associated with average wool fiber diameter, wool fineness SD, CV of fiber diameter, and wool crimp (P < 0.05). Our results suggest that FST influences wool quality traits and its SNPs 2 and 4 might be useful markers for marker-assisted selection and sheep breeding.

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Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Cited by 44 publications

References 19 publications

Variation in the ovine keratin-associated protein 15-1 gene affects wool yield

Variation in the ovine keratin-associated protein 15-1 gene affects wool yield

Identification of Caprine KRTAP28-1 and Its Effect on Cashmere Fiber Diameter

Polymorphisms of FST gene and their association with wool quality traits in Chinese Merino sheep

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