1992
DOI: 10.1111/j.1751-1097.1992.tb09731.x
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Identification of the Proton Acceptor of Schiff Base Deprotonation in Bacteriorhodopsin: A Fourier‐transform‐infrared Study of the Mutant Asp85 → Glu in Its Natural Lipid Environment

Abstract: Abstract— In order to assign the proton acceptor for Schiff base deprotonation in bacteriorhodopsin to a specific Asp residue, the photoreaction of the Asp85 → Glu mutant, as expressed in Halobacterium sp. GRB, was investigated by static low‐temperature and time‐resolved infrared difference spec‐troscopy. Measurements were also performed on the mutant protein labeled with [4‐13C]Asp which allowed discrimination between Asp and Glu residues. 14,15‐di13C‐retinal was incorporated to distinguish amide‐II absorban… Show more

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Cited by 60 publications
(68 citation statements)
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“…, characteristic of the M state (Braiman et al, 1988;Fahmy et al, 1992). As in many earlier reported spectra under these conditions, there is no obvious negative band that would indicate the deprotonation of Glu…”
supporting
confidence: 62%
“…, characteristic of the M state (Braiman et al, 1988;Fahmy et al, 1992). As in many earlier reported spectra under these conditions, there is no obvious negative band that would indicate the deprotonation of Glu…”
supporting
confidence: 62%
“…8,42 The FTIR spectra of D75E liposomes showed the same pattern of carboxylic acid protonation as in the wild-type ASR, displaying a predominance of the 1725 cm K1 or the 1716 cm K1 bands at the appropriate pH values, even though the 1716 cm K1 protonation band of Asp217 could be observed at pH values lower than those in the wildtype (not shown).…”
Section: Proton Transfers In Anabaena Rhodopsinmentioning
confidence: 67%
“…34, No. 41, 1995 13505 mode at 1760 cm-I, due to protonation of Asp85 (Braiman et al, 1988a;Fahmy et al, 1992), indicates that some M has been formed (compare to Figure 2c). Consequently, the spectrum of 9-H-BR550 taken at 213 K ( Figure IC) contains a mixture of L and M intermediates.…”
Section: Resultsmentioning
confidence: 85%