This study aims to contribute to the empirical literature on sustainable entrepreneurial ecosystems by understanding the opportunities and constraints to support its development using the case of Qatar. This study was designed using a triangulation method to combine different data collection techniques to increase the validity and reliability of the results. The data collection incorporated multiple data sources, starting with secondary sources and then collecting primary data through 37 interviews with key informants, mainly start-up founders and key stakeholders, a technique previously used in studies of critical players in entrepreneurial ecosystems. The findings were four-fold: (1) entrepreneurial ecosystem conditions are essential as facilitators of entrepreneurship, innovation, and sustainability, but government intervention can inhibit the outputs if the policies are not designed as customer-centric, (2) business sophistication is fundamental to increase innovation and attractiveness for investors but requires a stronger academic, industry, and government collaboration, (3) knowledge and technology outputs are limited when the domestic market is small, and the knowledge transfer policies are complex, and (4) the sustainability of an entrepreneurial ecosystem is fostered by the exposure to a crisis, robust national culture, and joint vision to reach sustainable development. This study provides evidence that shows a positive relationship between innovation and sustainable economic development, which makes this research even more relevant to our aim of supporting the Qatar National Vision 2030; at the same time, it contributes to the GCC literature and guides policymakers in the region.