Identification of the rice D-genome chromosomes by genomic in situ hybridisation

Cytogenetic comparisons between A and G genomes in Oryza using genomic in situ hybridization

“…The different hybridization stringencies and the rates of blocking DNA used in GISH procedures, and the fluorescence intensities observed be- minuta, indicated that co-hybridization existed between C and D genomes and B and C genomes [13]. Under the different stringent washing conditions, the multicolor GISH results indicated that the C, D and E genomes shared a substantial amount of similar sequences [5].…”

“…The GISH method has been used to study differentiation and relationships among the A, B, C, D and E genomes of the genus Oryza [5,[13][14][15][16][17]. The different hybridization stringencies and the rates of blocking DNA used in GISH procedures, and the fluorescence intensities observed be- minuta, indicated that co-hybridization existed between C and D genomes and B and C genomes [13].…”

“…It has played a significant role in understanding genome origin and evolution in the genus Oryza [5,[13][14][15][16][17]. GISH was used to study relationships among the B, C and D genomes, and the result suggested that the D genome was closer to the C than to the B genome [13].…”

“…It has played a significant role in understanding genome origin and evolution in the genus Oryza [5,[13][14][15][16][17]. GISH was used to study relationships among the B, C and D genomes, and the result suggested that the D genome was closer to the C than to the B genome [13]. The differentiation and relationships among the C, D and E genomes in the O. officinalis complex were detected by multicolor GISH, which indicated that the C, D and E genomes shared a substantial amount of similar sequences, and differentiation between the D and C genomes of O. alta was less than that between the E genome and each of the C and D genomes [5].…”

Painting of Parental Chromatin in Beta Hybrids by Multi-colour Fluorescent in situ Hybridization

“…GISH has been applied for the identi®cation of parental genomes in natural polyploids such as Triticum aestivum, Milium montianum and other plant species (Bennett et al, 1992;Mukai et al, 1993;Fukui et al, 1997) and in ornamental plants (érgaard et al, 1995;Kuipers et al, 1997). The detection of alien chromatin in interspeci®c crop hybrids has been described and, at higher resolution, chromosomal rearrangements such as translocations of chromosome arms or segments are detectable (HeslopHarrison et al, 1990;Schwarzacher et al, 1992;Kosina and Heslop-Harrison, 1996).…”