Identification of the S-Endo 1 Endothelial-Associated Antigen

Bardin, Nathalie; Francès, V.; Lesaule, G.; Horschowski, N; George, F.; Sampol, José

doi:10.1006/bbrc.1996.0037

Cited by 118 publications

(101 citation statements)

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“…Expression of CD146 is known to be limited to endothelial cells (1,16,17) and a subset of activated T-cells (11,18). Two mAb derived from the P1H12 clone (one from BD Biosciences and one from Chemicon [Temecula, CA]), and a third mAb derived from the S-Endo 1 clone (BioCytex [Marseille, France]) were titrated on HUVEC, CMOIC, and lymphocytes (CD45 bright , SSC low ).…”

Section: Cmoic Do Not Express Cd146mentioning

confidence: 99%

Cells meeting our immunophenotypic criteria of endothelial cells are large platelets

Strijbos

Kraan

Bakker

et al. 2007

Cytometry Part B Clinical

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Section: Cmoic Do Not Express Cd146mentioning

confidence: 99%

Cells meeting our immunophenotypic criteria of endothelial cells are large platelets

Strijbos

Kraan

Bakker

et al. 2007

Cytometry Part B Clinical

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“…Using S-Endo1 mAb, we previously detected cell surface expression of CD146 on human vascular endothelial cells, whatever vessel caliber or anatomical region [10]. On cultured endothelial cells, CD146 is mainly located at the intercellular junctions and is involved in inter-endothelial cell adhesion and paracellular permeability [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…The adhesive functions of CD146 were demonstrated using a solid-phase adhesion test in which both CD146-positive and CD146-negative melanoma cells bound to eucaryotic recombinant CD146 protein, suggesting that CD146 can mediate both homophilic and heterophilic interaction with an as yet unidentified ligand [8,9]. Using S-Endo1 mAb, we previously detected cell surface expression of CD146 on human vascular endothelial cells, whatever vessel caliber or anatomical region [10]. On cultured endothelial cells, CD146 is mainly located at the intercellular junctions and is involved in inter-endothelial cell adhesion and paracellular permeability [11,12].…”

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confidence: 99%

Mouse CD146/MCAM is a marker of natural killer cell maturation

et al. 2008

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CD146/melanoma cell adhesion molecule is an adhesion molecule expressed by endothelial cells and by a small fraction of activated T and B lymphocytes in humans. In order to analyze the pattern of CD146 expression in mouse leukocytes at steady-state conditions, we generated a set of novel rat anti-mouse CD146 monoclonal antibodies. CD146 expression was undetectable on monocytes, dendritic cells, T cells or B cells, but was expressed on about 30% of neutrophils and 60% of NK cells. Within murine lymphocytes, CD146 was defined as a novel NK-specific surface molecule. An increased percentage of CD146 1 cells was found in the most mature CD27 À CD11b 1 NK cell subpopulation, which also displays higher expression of Ly49C/I, Ly49D and KLRG1 and lower expression of NKG2A/C/E molecules. CD146 1 NK cells were found to be less cytotoxic and produce less IFN-c than CD146 À NK cells upon stimulation with target cells or activating antibodies. These findings define CD146 as a marker of mouse NK cell maturation that may be used as an alternative to the combined use of CD27 and CD11b staining to detect final stages of NK cell maturation.Key words: Adhesion . Antibodies . CD146/MCAM . Maturation . NK cells Introduction CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, A32 antigen, is an integral membrane glycoprotein of 113 kDa that belongs to the Ig superfamily with a characteristic V-V-C2-C2-C2 domain structure [1]. Two others members of this family have been identified in humans: basal-cell adhesion molecule and activated leukocytecell adhesion molecule, also called CD166 [2,3]. Basal-cell adhesion molecule is a splice variant of the Lutheran blood group glycoprotein that is reported to bind laminin [3]. Activated leukocyte-cell adhesion molecule is a costimulatory molecule mediating homophilic interaction and heterophilic interaction with CD6 and is involved in activation and trafficking of leukocytes into the central nervous system [4]. CD146 was originally defined as a marker of tumor progression and metastasis formation in human melanoma [5]. Overexpression of CD146 in CD146-negative melanoma cells renders these cells highly metastatic after injection in immunodeficient mice [6]. Moreover, injection of a humanized anti-CD146 mAb in immunodeficient mice bearing human melanomas significantly reduces the tumor size and the number of metastases [7]. The adhesive functions of CD146 were demonstrated using a solid-phase adhesion test in which both CD146-positive and CD146-negative melanoma cells bound to eucaryotic recombinant CD146 protein, suggesting that CD146 can mediate both homophilic and heterophilic interaction with an as yet unidentified ligand [8,9]. Using S-Endo1 mAb, we previously detected cell surface expression of CD146 on human vascular endothelial cells, whatever vessel caliber or anatomical region [10]. On cultured endothelial cells, CD146 is mainly located at the intercellular junctions and is involved in inter-endothelial cell adhesion and paracellular permeability [11,1...

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“…CD146 (also referred to as MUC18, MCAM, Mel-CAM, S-Endo-1, P1H12 antigen) was initially identified as a marker of tumour progression and metastasis formation in human melanoma (Lehmann et al, 1989;Bardin et al, 1996;Shih et al, 1997). CD146 is homologous to several cell adhesion molecules and belongs to the immunoglobulin superfamily containing five extracellular immunoglobulin-like domains, a single transmembrane domain and a short cytoplasmic domain (Sers et al, 1993).…”

mentioning

confidence: 99%

“…CD146 is homologous to several cell adhesion molecules and belongs to the immunoglobulin superfamily containing five extracellular immunoglobulin-like domains, a single transmembrane domain and a short cytoplasmic domain (Sers et al, 1993). Beside its expression in malignant melanocytes, CD146 is constitutively expressed in all endothelial cells, irrespective of the anatomical localisation (Bardin et al, 1996).…”

mentioning

confidence: 99%

Real-time PCR of CD146 mRNA in peripheral blood enables the relative quantification of circulating endothelial cells and is an indicator of angiogenesis

et al. 2005

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Angiogenesis is a fundamental process in tumour growth and metastatic dissemination. Possible surrogate markers for tumour angiogenesis are the amounts of circulating endothelial cells (CEC) in peripheral blood and the plasma concentration of vascular endothelial growth factor (VEGF). We tested the suitability of real-time PCR for CD146, an endothelial cell-specific antigen, to quantify CEC numbers in comparison to a flow cytometry quantification. Real-time PCR of CD146 mRNA showed high sensitivity and linearity for the quantification of cultivated primary endothelial cells added in different amounts to blood samples. Circulating endothelial cell numbers were quantified in peripheral blood samples of breast cancer patients and healthy controls by four-colour flow cytometry analysis and CD146 real-time PCR, and VEGF plasma concentrations were measured by ELISA. The amounts of CEC detected with both methods correlated significantly and CEC numbers were significantly increased in newly diagnosed breast cancer patients compared to healthy controls. Vascular endothelial growth factor concentrations correlated significantly with CEC numbers, but there was no significant difference in VEGF levels between breast cancer patients and healthy controls indicating that VEGF plasma levels cannot be used as surrogate marker for tumour angiogenesis. Taken together, the quantification of CEC by CD146 realtime PCR showed equivalent results to the flow cytometry analysis. Thus, CD146 real-time PCR may be an easy and reliable approach to quantify CEC in peripheral blood samples and could facilitate the integration of CEC measurements in clinical studies exploring the efficacy of antiangiogenic therapies.

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Identification of the S-Endo 1 Endothelial-Associated Antigen

Cited by 118 publications

References 0 publications

Cells meeting our immunophenotypic criteria of endothelial cells are large platelets

Cells meeting our immunophenotypic criteria of endothelial cells are large platelets

Mouse CD146/MCAM is a marker of natural killer cell maturation

Real-time PCR of CD146 mRNA in peripheral blood enables the relative quantification of circulating endothelial cells and is an indicator of angiogenesis

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