Identification of the similarly expressed genes in patients with polycystic ovary syndrome and transsexuals

Abstract

Polycystic ovary syndrome (PCOS) is a common female infertility, which may be caused by excessive androgen, but its mechanism remains unknown. Transsexuals are women who take androgen drugs for a long time, and gradually have male signs. Their ovaries may have received high concentrations of androgen, which leads to the failure of ovarian reproductive function. Therefore, we searched the relevant data of PCOS and transsexuals in gene expression omnibus database, used limma package to identify the most similarly genes, and then analyzed the possible mechanism of PCOS through gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. Then, the protein-protein interaction network was constructed by searching the String database, and the top 5 hub genes were identified by the cytohubba plug-in of Cytoscape. Finally, ubiquitin conjugating enzyme E2 E1 (UBE2E1), ubiquitin C (UBC), transcription elongation factor B subunit 1 (TCEB1), ubiquitin conjugating enzyme E2 N (UBE2N), and ring finger protein 7 (RNF7) genes were identified as the most similarly expressed genes between PCOS and Transsexuals. They may cause the ubiquitination of androgen receptor and eventually lead to sinus follicular growth arrest. In conclusion, 5 Central genes were identified in PCOS and transsexuals. These genes can be used as targets for early diagnosis or treatment of PCOS.

An interactive analysis of the mouse oviductal miRNA profiles

“…Regarding the reproductive system, three of these genes ( UBE2N, PPP2R1B and ACVR2A ) have been shown to be involved in reproductive disfunctions in humans (polycystic ovary syndrome and transsexuality, azoospermia, and susceptibility to preeclampsia, respectively ( Fitzpatrick et al, 2009 ; Dong et al, 2021 ; Du et al, 2021 ). So far, a total of 15 genes out of 21 ( UBE2N, PPP2R1B, CACNA1C, AP2B1, NCOA2, RAC1, MED1, PLOD2, GNA13, AFDN, CCND2, BMI1, PLCB1, DNMT3A and SKP2) have been associated to tumors of the female or male reproductive tracts (cervical carcinoma, ovarian, endometrial, prostate, and testicular cancers ( Tables 4 and 5 ), in addition to other types of cancers or diseases.…”

Genome-Wide Association Studies and Runs of Homozygosity to Identify Reproduction-Related Genes in Yorkshire Pig Population

“…The genes found to be associated with the DEAD trait include the ring finger protein 7 (RNF7), G protein-coupled receptor kinase (GRK7), and ets variant gene 5 (ETV5). The RNF7 gene, identified as the gene most similar to the expression of PCOS in transgender people, may lead to ubiquitination of the androgen receptor and eventually lead to antral follicular growth stagnation [72]. This may affect the mother's conception and pregnancy, which is not good for the fetus.…”