Identification of Three Novel HIV-1 Second-Generation Recombinant Forms (CRF01_AE/CRF07_BC) Among Men Who Have Sex with Men in Baoding, Hebei, China

Fan, Weiguang; Liu, Yang; Li, Yan; Su, Miaomiao; Meng, Juan; Lu, Xinli; Shi, Penghui

doi:10.1089/aid.2022.0037

Cited by 7 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In conclusion, we identified two almost identical HIV-1 URFs among MSMs in Baoding. The recombinant forms of CRF01_AE/B and CRF01_AE/CRF07_BC were found frequently in the MSM population of Baoding, indicating an increase in the genetic diversity of HIV-1 and the presence of many undiagnosed multiple infection cases in this region ( Ou et al, 2019 ; Fan et al, 2022d ; Yang et al, 2022 ), further bringing more barriers for HIV prevention and therapy. This study suggests that we should continuously monitor HIV-1 molecular epidemiology in order to provide effective suggestions to HIV-1 prevention and vaccine design.…”

Section: Discussionmentioning

confidence: 99%

Identification of two near-identical novel HIV-1 unique recombinant forms (CRF01_AE/B) among men who have sex with men in baoding, hebei, China

Zhang

Chen²,

Meng³

et al. 2023

Front. Genet.

Self Cite

View full text Add to dashboard Cite

Men who have sex with men (MSM) are the most frequent infection route of the human immunodeficiency virus (HIV) in Baoding, China, creating chances for the occurrence of unique recombinant forms (URFs) of the virus, i.e., recombination of different subtypes caused by co-circulation of multiple subtypes. In this report, two near-identical URFs (BDD002A and BDD069A) isolated from MSM in Baoding were identified. Phylogenetic tree analysis based on nearly full-length genomes (NFLGs) revealed that the two URFs formed a distinct monophyletic cluster with a bootstrap value of 100%. Recombinant breakpoints analysis identified that the NFLGs of BDD002A and BDD069A were both composed of CRF01_AE and subtype B, with six subtype B mosaic segments inserted into the CRF01_AE backbone. The CRF01_AE segments of the URFs clustered closely with the CRF01_AE reference sequences, and the B subregions clustered with the B reference sequences. The recombinant breakpoints of the two URFs were almost identical. These results suggest that effective interventions are urgently needed to prevent the formation of complex HIV-1 recombinant forms in Baoding, China.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of two near-identical novel HIV-1 unique recombinant forms (CRF01_AE/B) among men who have sex with men in baoding, hebei, China

Zhang

Chen²,

Meng³

et al. 2023

Front. Genet.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The convenient transport network in this region enables geographical mobility, and creates opportunities for dual or multiple infections within the MSM population. This has led to the emergence and prevalence of new recombinant strains in MSM in recent years, such as CRF103_01B, CRF123_0107, CRF01_AE/B and CRF01_AE/CRF07_BC ( Huang et al, 2019 ; Zhou et al, 2020 ; Han et al, 2021 ; Xing et al, 2021 ; Fan et al, 2022a , b ; Xing et al, 2022 ). The emergence of new recombinant forms have increased the diversity of HIV-1 isolates prevalent in Hebei province, indicating the further molecular monitoring of HIV-1 diversity is vital in the region.…”

Section: Discussionmentioning

confidence: 99%

Characterization of two novel HIV-1 second-generation recombinants (CRF01_AE/CRF07_BC) identified in Hebei Province, China

Yang

Zhao

et al. 2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

IntroductionThe unique recombinant forms (URFs) of HIV-1 consist of a mixture of subtypes, and each URF has a unique breakpoint. In this study, we identified the near fulllength genome (NFLG) sequences of two novel HIV-1 URFs (Sample ID: BDD034A and BDL060) isolated during HIV-1 molecular surveillance in 2022 in Baoding city, Hebei Province, China.MethodsThe two sequences were aligned with subtype reference sequences and CRFs from China using MAFFT v7.0, and the alignments were adjusted manually using BioEdit (v7.2.5.0). Phylogenetic and subregion trees were constructed using MEGA11 with the neighbor-joining (N-J) method. Recombination breakpoints were identified by SimPlot (v3.5.1) based on Bootscan analyses.ResultsRecombinant breakpoint analysis revealed that the NFLGs of BDD034A and BDL060 were composed of CRF01_AE and CRF07_BC, containing seven segments, respectively. For BDD034A, three CRF01_AE fragments were inserted into the CRF07_BC main framework, whereas for BDL060, three CRF07_BC fragments were inserted into the CRF01_AE main framework.DiscussionThe emergence of the CRF01_AE/CRF07_BC recombinant strains indicates that HIV-1 co-infection is common. The increasing genetic complexity of the HIV-1 epidemic in China warrants continued investigation.

show abstract

“…Inter-subtype recombinant viruses, especially CRF01_AE and CRF07_BC, have become the predominant strains of the HIV-1 epidemic in China [ 10 ]. The double infection between CRFs established more optimal conditions for the second-generation recombination, and many second-generation recombinants were represented by the recombination of CRF01_AE and CRF07_BC fragments, such as CRF79_0107 [ 11 ], CRF80_0107 [ 12 ], CRF102_01075 [ 13 ], CRF109_0107 [ 14 ], CR125_0107 [ 15 ], and others [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. Other instances include CRF01_AE x CRF08_BC [ 25 ], CRF01_AE x subtype B [ 26 ], CRF07_BC x CRF83_cpx [ 27 ], and CRF86_BC x a URF [ 28 ] also from in China.…”

mentioning

confidence: 99%

Second-Generation SARS-CoV-2 Recombinants: Lessons from Other Viruses

Focosi¹,

Maggi

2023

Viruses

View full text Add to dashboard Cite

show abstract

Identification of Three Novel HIV-1 Second-Generation Recombinant Forms (CRF01_AE/CRF07_BC) Among Men Who Have Sex with Men in Baoding, Hebei, China

Cited by 7 publications

References 12 publications

Identification of two near-identical novel HIV-1 unique recombinant forms (CRF01_AE/B) among men who have sex with men in baoding, hebei, China

Identification of two near-identical novel HIV-1 unique recombinant forms (CRF01_AE/B) among men who have sex with men in baoding, hebei, China

Characterization of two novel HIV-1 second-generation recombinants (CRF01_AE/CRF07_BC) identified in Hebei Province, China

Second-Generation SARS-CoV-2 Recombinants: Lessons from Other Viruses

Contact Info

Product

Resources

About