Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region

Toda, Satoshi; Komazaki, Shinkichi

doi:10.1079/ber2002177

Cited by 79 publications

(60 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Total genomic DNA was extracted from each single female and male adult thrips after Toda and Komazaki (2002). Thrips were homogenized in 100 ml of lysis buffer (100 mM Tris-HCl (pH 8.0), 100 mM EDTA (pH 8.0), 1% sodium dodecyl sulfate (SDS), 0.1 mg/ml proteinase K) and incubated for 10 min at 65°C.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic analysis based on mitochondrial COI gene sequences in Thrips tabaci Lindeman (Thysanoptera: Thripidae) in relation to reproductive forms and geographic distribution

Toda

Murai

2007

Appl. Entomol. Zool.

View full text Add to dashboard Cite

Thrips tabaci Lindeman is an extensively distributed pest insect that injures a wide range of crops. To investigate the intra-specific genetic diversity of this species, we analyzed an 810 bp region of the mitochondrial cytochrome oxidase gene subunit I (COI). Eight populations from six foreign countries and 18 Japanese populations were tested, and 17 different haplotypes were identified. Apparent differences were found between arrhenotokous and thelytokous strains in their COI sequences. A phylogenetic tree of the COI gene shows two distinct groups. We assume that these two groups correspond respectively to the arrhenotokous strain and the thelytokous strain. Japanese thelytokous types consisted of five haplotypes. Two haplotypes were identified from problematic populations in terms of the greater amount of damage they caused and the development of insecticide resistance. Both haplotypes were also found overseas, suggesting that some strains from overseas may have caused the above-mentioned problems in Japan.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Japanese T. tabaci samples were collected from a variety of host plants in 18 localities (Table 1). Species were identified by observation of morphological characteristics and a molecular diagnostic method (Toda and Komazaki, 2002). The reproductive form was identified in 13 populations which were offered in a viable condition.…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic analysis based on mitochondrial COI gene sequences in Thrips tabaci Lindeman (Thysanoptera: Thripidae) in relation to reproductive forms and geographic distribution

Toda

Murai

2007

Appl. Entomol. Zool.

View full text Add to dashboard Cite

show abstract

“…Molecular approaches have been advanced in respect to the identification of species in the genera of Frankliniella and Thrips (Brunner et al, 2002;Toda and Komazaki, 2002). Analysis of mitochondrial and nuclear DNA would be a powerful tool to investigate the phylogenetic relationships among thrips vectors of Tospovirus.…”

Section: Introductionmentioning

confidence: 99%

The phylogeny of thrips (Thysanoptera: Thripidae) based on partial sequences of cytochrome oxidase I, 28S ribosomal DNA and elongation factor-1 α and the association with vector competence of tospoviruses

Inoue

Sakurai

2007

Appl. Entomol. Zool.

View full text Add to dashboard Cite

The phylogenetic relationships among 16 species including 10 thrips vector species of tospoviruses were analysed using partial sequences of three genes: mitochondrial COI, nuclear 28S ribosomal DNA and the elongation factor-1a (EF-1a) in order to reveal the influence of lineage among thrips vector species on the vector competence of tospoviruses. The phylogenetic trees of thrips suggested that distinct clades were formed between the genera Frankliniella and Thrips including vector species at least in combined nuclear DNA data, although COI data showed a similar result despite low bootstrap value in the root of Thrips clade. Scirtothrips had an ambiguous phylogenetic position in this study. Vector and non-vector species were found within the same clade in the genus Thrips. Compared with the phylogenetic tree of thrips, the virus phylogeny inferred from the nucleocapsid (N) protein sequence were likely to correspond to the phylogeny of thrips, genera Frankliniella, Thrips and Scirtothrips, respectively, and also to the primary host plants under natural conditions. These results suggested that the vector competence of thrips for tospoviruses might be influenced by the lineages of thrips vectors as well as host plants.

show abstract

“…DNA-based analysis can be applied to identify different thrips at all life stages. Several polymerase chain reaction-restriction fragment-length polymorphism (PCR -RFLP) protocols have been developed to separate selected groups of thrip species, including F. occidentalis (Brunner et al 2002;Toda & Komazaki 2002). A real-time PCR-based method was developed for identification of the melon thrips (Thrips palmi; Walsh et al 2005), but this study did not include F. occidentalis.…”

Section: Introductionmentioning

confidence: 99%

Taqman real-time quantitative PCR for identification of western flower thrip ( Frankliniella occidentalis ) for plant quarantine

et al. 2010

View full text Add to dashboard Cite

These authors contributed equally to the study. Western flower thrip (Frankliniella occidentalis)is a major global pest of agricultural products. It directly damages crops through feeding, oviposition activity or transmission of several plant viruses. We describe a Taqman real-time quantitative PCR detection system, which can rapidly identify F. occidentalis from thrips larvae to complement the traditional morphological identification. The data showed that our detection system targeted on the ribosomal RNA gene regions of F. occidentalis has high sensitivity and specificity. The rapid method can be used for on-site testing of samples at ports-of-entry in the future.

show abstract

Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region

Cited by 79 publications

References 15 publications

Phylogenetic analysis based on mitochondrial COI gene sequences in Thrips tabaci Lindeman (Thysanoptera: Thripidae) in relation to reproductive forms and geographic distribution

Phylogenetic analysis based on mitochondrial COI gene sequences in Thrips tabaci Lindeman (Thysanoptera: Thripidae) in relation to reproductive forms and geographic distribution

The phylogeny of thrips (Thysanoptera: Thripidae) based on partial sequences of cytochrome oxidase I, 28S ribosomal DNA and elongation factor-1 α and the association with vector competence of tospoviruses

Taqman real-time quantitative PCR for identification of western flower thrip ( Frankliniella occidentalis ) for plant quarantine

Contact Info

Product

Resources

About