Identification of timothy cultivars by SDS-PAGE analysis of seed storage proteins

Cai, Qian-Rong; Bullen, M. R.

doi:10.4141/cjps92-148

Cited by 4 publications

(5 citation statements)

References 6 publications

(1 reference statement)

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“…SDS-PAGE of seed proteins of a bulked seed sample has proved to be an effective method for distinguishing cultivars of largely outbreeding grasses, legumes and sugar beet (Ferguson & Grabe, 1986;Gardiner & Forde, 1992;Cai & Bullen, 1992;Oleo et al, 1992;M611er & Spoor, 1993) but the present study is the first report on buckwheat cultivar identification. Studies of allozymes in common buckwheat (Ohnishi, 1993) have shown high genetic variability within cultivats and cultivated populations and small variability of allele frequencies among them.…”

Section: Discussionmentioning

confidence: 75%

Seed protein variation for identification of common buckwheat (Fagopyrum esculentum Moench) cultivars

Rogl

Javornik

1996

Euphytica

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Total seed proteins of 24 common buckwheat cultivars and cultivated populations within a molecular weight range of 30 to 54 kDa were analysed by SDS-PAGE. Single seed analysis of six cultivars identified a total of 18 alternative protein bands with different mobilities. Differences of individual protein band frequencies extracted from single seeds among six buckwheat cultivars varied distinctively, indicating high intravarietal polymorphism. The relation between frequencies of protein bands revealed by single seed analysis and their appearance on the bulk seed analyses was demonstrated. Regarding to band mobility rate and relative band intensity among 24 bulk samples analysed, 14 had distinctive electrophoregrams while the other 10 were ranged into four distinct groups. Analysis of endosperm and cotyledon proteins showed that proteins stored in these main seed parts are tissue specific. The observed electrophoretic polymorphism related to proteins stored in the cotyledons while there was no apparent variability with endosperm proteins.

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Section: Discussionmentioning

confidence: 75%

Seed protein variation for identification of common buckwheat (Fagopyrum esculentum Moench) cultivars

Rogl

Javornik

1996

Euphytica

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“…Values of p˂ 0.05 were considered to be statistically significant. A great deal of attention and efforts have been paid to the development of biochemical methods for cultivar characterization, variety discrimination and identification (Gardiner et al, 1986;Cai and Bulletin 1992;El-Kariem 2002, 2003;Oladipo et al, 2008). The most widely applied and versatile technique used for laboratory cultivar characterization has been the analysis of seed and leaf proteins and enzymes by various forms of electrophoresis.…”

Section: Discussionmentioning

confidence: 99%

“…However, the protein concentrations of the leaf seedling of cultivar from North Central zone was statistically lower than other zones. Studies have revealed that proteins are the products of structural genes and could also be influenced by environmental factors, application of fertilizers and other external conditions (Cai and Bulletin 1992;Gottlieb 1972).…”

Section: Discussionmentioning

confidence: 99%

“…It is employed to detect differences in polymorphic proteins in different cultivars. Since protein composition is genetically determined, it is not subjected to environmental conditions as well as the location of the genes coding for particular proteins in several cases (Aiken andGardiner 1991, Cai andBulletin 1992). Electrophoretic analyses of proteins have advantages over traditional identification techniques in that it offers a rapid and reliable evaluation of genetic differences among most cultivars (Ferguson and Grabe 1986, Gardiner and Fered 1987, 1988, it is a promising tool to distinguish cultivars of a particular crop species (Illoh 1990, Moller and Spoor 1993, Jha and Ohri 1996, Essiet and Illoh 1997, Folorunso and Olorode, 2002.…”

Section: Introductionmentioning

confidence: 99%

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2014

GJBS

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Thirty-five (35), wild (5) and cultivated (30) Sorghum bicolor germplasms were collected from 5 different agro-ecological zones in Nigeria. Proteins were extracted from six weeks old fresh leaves (1.0 g) with 10ml of 0.85% (w/v) sodium chloride (NaCl) solution, filtered and followed by centrifugation at room temperature at 3,000 rpm for 10 min. The supernatants were employed for protein analyses on polyacrylamide gel electrophoresis in the presence of SDS. The protein concentrations of the cultivars from North East, North West, South and wild were not statistically different ( P < 0.05) but in the same range with those of cultivars from North Central agro-ecological zone. Analyses of proteins revealed a total of 141 protein bands produced by the cultivars, 21 % has relative molecular mass of 190kD, 24% (22kD), 21% (11kD) and 24% (10kD). However, proteins with 21kD and 26kD were specific to only cultivars from North Central and wild collections. The proportion of differentiated protein (Pp) ranged between 0.06 and 0.24 while the dendrogram constructs revealed little variation between the cultivated and wild sorghum accessions.

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“…The basic principle of evaluation is qualitative banding differences, i.e., the presence/absence of a band of a particular molecular mass. SDS-PAGE of bulked seed protein samples has proven to be an effective method for distinguishing cultivars of largely outbreeding grasses, legumes, and sugar beets [1,2,3,4,5]. In buckwheat, too, several reports are available.…”

Section: Introductionmentioning

confidence: 99%

Screening and Selection of Hypoallergenic Buckwheat Species

Nair¹,

Adachi

2002

The Scientific World JOURNAL

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Both common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed that F. lineare and F. urophyllum lack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species.

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Identification of timothy cultivars by SDS-PAGE analysis of seed storage proteins

Cited by 4 publications

References 6 publications

Seed protein variation for identification of common buckwheat (Fagopyrum esculentum Moench) cultivars

Seed protein variation for identification of common buckwheat (Fagopyrum esculentum Moench) cultivars

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Screening and Selection of Hypoallergenic Buckwheat Species

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