Identification of Toxicity Parameters Associated with Combustion Produced Soot Surface Chemistry and Particle Structure by in Vitro Assays

Housseiny, Heba Al; Singh, Madhu; Emile, Shaneeka; Nicoleau, Marvin; Wal, Randy Vander; Silveyra, Patricia

doi:10.3390/biomedicines8090345

Cited by 17 publications

(6 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the study of Totlandsdal et al (2010) , a concentration of 100 g/ml carbon exposure increased mRNA and protein expression of IL-1β and IL-6 in primary lung epithelial cells. Al Housseiny et al (2020) also said that IL-6 expression was increased in the bronchial tree during PM exposure ( Totlandsdal et al , 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Curcuma longa supplementation reduce MDA, TNF-α, and IL-6 levels in a rat model exposed to soot particulates

Aminuddin¹,

Sargowo²,

Sardjono³

et al. 2023

Open Vet J

View full text Add to dashboard Cite

Background: Particulate Matter (PM) is one of the important components in air pollution that can cause endothelial vascular dysfunction through exacerbation of atherosclerosis and inflammation of the respiratory system. Increased levels of MDA in blood plasma can be an indicator of oxidative stress. Then, macrophages can secrete proinflammatory cytokines will stimulate immune cells and vascular endothelial cells to release inflammatory cytokines, such as interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α). Curcuma longa works by scavenging the active free radicals involved in the peroxidation process. Aims: This study aims to prove that the administration of Curcuma longa can reduce MDA, TNF-α, and IL-6 levels in Rattus novergicus exposed to soot particulates. Methods: The subjects of this study were 30 male rats which were divided into 5 treatment groups with the following: (C0): negative control; (C+): positive control; (T1):Treatment group 2, rats exposed to particulate soot at a concentration of 1064 mg/m3 for 8 hours and given Curcuma longa at a dose of 1 mg/kg bw; (T2): Treatment group 3 was rats exposed to soot particulates at a concentration of 1064 mg/m3 for 8 hours and given Curcuma longa at a dose of 2 mg/kg bw; (T3) :Treatment group 4 was rats exposed to soot particulates at a concentration of 1064 mg/m3 for 8 hours and given Curcuma longa at a dose of 3 mg/kg bw. Giving the Curcuma longa extract orally with a probe every day for 30 days after treatment of exposure to soot. Examination of MDA, TNF-, and IL-6 levels with the ELISA method. Results: The administration of Curcuma longa can reduce MDA while the lowest MDA levels were obtained in the T3 treatment with an average of 1.542 ± 0.231. The results of the description of the lowest levels of TNF-α were obtained in the C-treatment with an average of 55.981 ± 4.689. Then, the lowest levels of IL-6 were obtained in the C-treatment with an average of 2.292±0.461. Conclusion: The results stated that the administration of Curcuma longa could reduce MDA levels, TNF-α, and IL-6 levels. Curcuma longa as an anti-inflammatory and anti-oxidant play an effective role in inhibiting inflammation by decreasing IL-6 cytokine and TNF-α. Curcuma longa can inhibit lipid peroxidation initiated by free radicals then reduce MDA levels.

show abstract

Section: Discussionmentioning

confidence: 99%

Curcuma longa supplementation reduce MDA, TNF-α, and IL-6 levels in a rat model exposed to soot particulates

Aminuddin¹,

Sargowo²,

Sardjono³

et al. 2023

Open Vet J

View full text Add to dashboard Cite

show abstract

“…The participation of the nuclear factor erythroid 2-related factor 2 (Nrf2) in the oxidative stress triggered by PM has been extensively reported in recent years (Badran et al, 2020;Bagam et al, 2021;Housseiny et al, 2020;Ito et al, 2020;Liu et al, 2022b;Saha et al, 2022;Shi et al, 2023;Shrestha et al, 2021;Son et al, 2020;Tian et al, 2021;Wang et al, 2022aWang et al, , 2022bWang et al, , 2022cXue et al, 2021b). This is not surprising given the key role of Nrf2 in the protection against oxidative stimuli (Saha et al, 2020), acting as a sensor to protect the cells against oxidative damage induced by PM-generated ROS.…”

Section: Nrf2: the Master Regulator Of Antioxidant Responsementioning

confidence: 96%

Particulate Matter-Induced Oxidative Stress – Recent Mechanistic Insights from In Vitro Studies

Vilas-Boas,

Chatterjee,

Carvalho

et al. 2024

Preprint

View full text Add to dashboard Cite

Inhaled particulate matter (PM) is a key player in the millions of yearly deaths worldwide related to air pollution. The oxidative potential of PM provides an indication of its ability to promote an oxidative environment. When the human lungs are exposed to PM, the reactive oxygen species (ROS) generated in such oxidative environment, if excessive, may lead to cell damage via oxidative stress. Inflammation, endoplasmic reticulum stress, airway remodeling, and many different cell death modes (apoptosis, ferroptosis, pyroptosis, among others) can be triggered by oxidative stress via complex molecular pathways, which also involve the adaptive cellular response. But ROS can also directly interact with macromolecules such as proteins and lipids, as well as nucleic acids, having been reported to induce DNA damage and epigenetic modifications, which jeopardizes homeostasis. These facts have been extensively studied in in vitro models and confirmed in in vivo models.In this review, we delve beyond conventional assessments of airborne particles, discussing their oxidative potential and the PM-induced ROS-mediated cellular damage observed in in vitro models. The close link between oxidative stress and inflammation, and the manifestation of different cell death modes is highlighted by reviewing the latest literature. The effects of ROS on the balance of DNA damage and repair, carcinogenicity and epigenetics interplay are then analyzed. Finally, we expand on the latest reports on the antioxidants’ potential to counteract the deleterious effects of ROS, and disclose future perspectives for the topic.

show abstract

“…In vitro cell studies have shown that exposure to soot, secondary organic aerosols (SOA) and diesel exhaust (DE) particles causes pro-inflammatory responses, including increased release of IL-6 and IL-8 [ 49 ]. Bronchial epithelial cells exposed to functionalized carbon black show increased mRNA levels of IL-1β and IL-6 [ 50 ] and bronchial epithelial cells exposed to SOA show increased levels of released IL-8 [ 51 ]. IL-6 from leucocytes, endothelial cells, keratinocytes and fibroblasts has been shown to play a significant role in acute inflammation and adaptive tissue remodelling [ 52 ].…”

Section: Respiratory Responses To Aerosol Exposurementioning

confidence: 99%

“…Altogether, IL-6 is acknowledged to be an important biomarker of the acute inflammatory response to particulate pollution. Elevated levels of IL-6 have been observed following PM exposure in in vitro [ 50 ] and in vivo [ 46 , 54 ] studies, as well as in blood samples from healthy volunteers under experimental and real-world exposure conditions [ 61 , 63 ]. This illustrates the relevance and coherence of in vitro and in vivo toxicology studies to real-world effects in humans.…”

Section: Respiratory Responses To Aerosol Exposurementioning

confidence: 99%

Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models

et al. 2023

View full text Add to dashboard Cite

Annually, an estimated seven million deaths are linked to exposure to airborne pollutants. Despite extensive epidemiological evidence supporting clear associations between poor air quality and a range of short- and long-term health effects, there are considerable gaps in our understanding of the specific mechanisms by which pollutant exposure induces adverse biological responses at the cellular and tissue levels. The development of more complex, predictive, in vitro respiratory models, including two- and three-dimensional cell cultures, spheroids, organoids and tissue cultures, along with more realistic aerosol exposure systems, offers new opportunities to investigate the cytotoxic effects of airborne particulates under controlled laboratory conditions. Parallel advances in high-resolution microscopy have resulted in a range of in vitro imaging tools capable of visualizing and analysing biological systems across unprecedented scales of length, time and complexity. This article considers state-of-the-art in vitro respiratory models and aerosol exposure systems and how they can be interrogated using high-resolution microscopy techniques to investigate cell–pollutant interactions, from the uptake and trafficking of particles to structural and functional modification of subcellular organelles and cells. These data can provide a mechanistic basis from which to advance our understanding of the health effects of airborne particulate pollution and develop improved mitigation measures.

show abstract

Identification of Toxicity Parameters Associated with Combustion Produced Soot Surface Chemistry and Particle Structure by in Vitro Assays

Cited by 17 publications

References 59 publications

Curcuma longa supplementation reduce MDA, TNF-α, and IL-6 levels in a rat model exposed to soot particulates

Curcuma longa supplementation reduce MDA, TNF-α, and IL-6 levels in a rat model exposed to soot particulates

Particulate Matter-Induced Oxidative Stress – Recent Mechanistic Insights from In Vitro Studies

Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models

Contact Info

Product

Resources

About